Roles of core nosZ denitrifiers in enhancing denitrification activity under long-term rice straw retention
The denitrification process is known to contribute to soil nitrogen (N) loss, which is strongly affected by fertilization strategies; however, the effects of distinct straw retention modes on soil denitrification activity have rarely been discriminated and the underlying mechanisms remain unclear. T...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1541202/full |
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| author | Shijie Zhang Shijie Zhang Mengyao Hou Mengyao Hou Bing Li Bing Li Panfeng Guan Panfeng Guan Qing Chi Qing Chi Hao Sun Hao Sun Hangbo Xu Hangbo Xu Dongjie Cui Dongjie Cui Yupan Zhu Yupan Zhu |
| author_facet | Shijie Zhang Shijie Zhang Mengyao Hou Mengyao Hou Bing Li Bing Li Panfeng Guan Panfeng Guan Qing Chi Qing Chi Hao Sun Hao Sun Hangbo Xu Hangbo Xu Dongjie Cui Dongjie Cui Yupan Zhu Yupan Zhu |
| author_sort | Shijie Zhang |
| collection | DOAJ |
| description | The denitrification process is known to contribute to soil nitrogen (N) loss, which is strongly affected by fertilization strategies; however, the effects of distinct straw retention modes on soil denitrification activity have rarely been discriminated and the underlying mechanisms remain unclear. This study coupled field and incubation experiments to explore the characteristics of soil denitrification activity, soil and standing water physicochemical properties, and the abundance, community diversity, and co-occurrence network of nosZ denitrifiers, based on a paddy field implementing 10-year straw retention under a rice–wheat rotation system. Four straw retention treatments with equivalent chemical fertilizers were applied, namely no straw (NS), wheat straw only (WS), rice straw only (RS), and wheat and rice straw (WRS). Results indicated a significant increase (by 41.93–45.80% when compared to that with NS) in the soil denitrification activity with RS and WRS. Correspondingly, treatments with rice straw retention resulted in the development of a similar community composition (P < 0.05), structure (P = 0.001), and more positively interconnected network, as well as similar specific keystone taxa of nosZ denitrifiers, relative to those in non-rice straw mode. Under long-term rice straw retention conditions, the core nosZ-denitrifying phylogroups shifted (r = 0.83, P < 0.001), with the recruitment of keystone taxa from the phyla Bacteroidetes and Euryarchaeota playing a key role in enhancing denitrification activity and stimulating N loss. Accordingly, in a rice–wheat rotation field, the practice of wheat straw retention in a single season is recommended because it will not markedly sacrifice soil N availability impaired by the denitrification process. |
| format | Article |
| id | doaj-art-7fd66225fd7e4fb68667c40680797dcd |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-7fd66225fd7e4fb68667c40680797dcd2025-02-07T06:49:47ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-02-011610.3389/fpls.2025.15412021541202Roles of core nosZ denitrifiers in enhancing denitrification activity under long-term rice straw retentionShijie Zhang0Shijie Zhang1Mengyao Hou2Mengyao Hou3Bing Li4Bing Li5Panfeng Guan6Panfeng Guan7Qing Chi8Qing Chi9Hao Sun10Hao Sun11Hangbo Xu12Hangbo Xu13Dongjie Cui14Dongjie Cui15Yupan Zhu16Yupan Zhu17Zhengzhou Research Base, State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, ChinaHenan Key Laboratory of Ion-Beam Green Agriculture Bioengineering, Zhengzhou University, Zhengzhou, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, ChinaHenan Key Laboratory of Ion-Beam Green Agriculture Bioengineering, Zhengzhou University, Zhengzhou, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, ChinaHenan Key Laboratory of Ion-Beam Green Agriculture Bioengineering, Zhengzhou University, Zhengzhou, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, ChinaHenan Key Laboratory of Ion-Beam Green Agriculture Bioengineering, Zhengzhou University, Zhengzhou, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, ChinaHenan Key Laboratory of Ion-Beam Green Agriculture Bioengineering, Zhengzhou University, Zhengzhou, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, ChinaHenan Key Laboratory of Ion-Beam Green Agriculture Bioengineering, Zhengzhou University, Zhengzhou, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, ChinaHenan Key Laboratory of Ion-Beam Green Agriculture Bioengineering, Zhengzhou University, Zhengzhou, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, ChinaHenan Key Laboratory of Ion-Beam Green Agriculture Bioengineering, Zhengzhou University, Zhengzhou, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, ChinaHenan Key Laboratory of Ion-Beam Green Agriculture Bioengineering, Zhengzhou University, Zhengzhou, ChinaThe denitrification process is known to contribute to soil nitrogen (N) loss, which is strongly affected by fertilization strategies; however, the effects of distinct straw retention modes on soil denitrification activity have rarely been discriminated and the underlying mechanisms remain unclear. This study coupled field and incubation experiments to explore the characteristics of soil denitrification activity, soil and standing water physicochemical properties, and the abundance, community diversity, and co-occurrence network of nosZ denitrifiers, based on a paddy field implementing 10-year straw retention under a rice–wheat rotation system. Four straw retention treatments with equivalent chemical fertilizers were applied, namely no straw (NS), wheat straw only (WS), rice straw only (RS), and wheat and rice straw (WRS). Results indicated a significant increase (by 41.93–45.80% when compared to that with NS) in the soil denitrification activity with RS and WRS. Correspondingly, treatments with rice straw retention resulted in the development of a similar community composition (P < 0.05), structure (P = 0.001), and more positively interconnected network, as well as similar specific keystone taxa of nosZ denitrifiers, relative to those in non-rice straw mode. Under long-term rice straw retention conditions, the core nosZ-denitrifying phylogroups shifted (r = 0.83, P < 0.001), with the recruitment of keystone taxa from the phyla Bacteroidetes and Euryarchaeota playing a key role in enhancing denitrification activity and stimulating N loss. Accordingly, in a rice–wheat rotation field, the practice of wheat straw retention in a single season is recommended because it will not markedly sacrifice soil N availability impaired by the denitrification process.https://www.frontiersin.org/articles/10.3389/fpls.2025.1541202/fullstraw retentiondenitrification activitynosZ genedenitrifierspaddy field |
| spellingShingle | Shijie Zhang Shijie Zhang Mengyao Hou Mengyao Hou Bing Li Bing Li Panfeng Guan Panfeng Guan Qing Chi Qing Chi Hao Sun Hao Sun Hangbo Xu Hangbo Xu Dongjie Cui Dongjie Cui Yupan Zhu Yupan Zhu Roles of core nosZ denitrifiers in enhancing denitrification activity under long-term rice straw retention Frontiers in Plant Science straw retention denitrification activity nosZ gene denitrifiers paddy field |
| title | Roles of core nosZ denitrifiers in enhancing denitrification activity under long-term rice straw retention |
| title_full | Roles of core nosZ denitrifiers in enhancing denitrification activity under long-term rice straw retention |
| title_fullStr | Roles of core nosZ denitrifiers in enhancing denitrification activity under long-term rice straw retention |
| title_full_unstemmed | Roles of core nosZ denitrifiers in enhancing denitrification activity under long-term rice straw retention |
| title_short | Roles of core nosZ denitrifiers in enhancing denitrification activity under long-term rice straw retention |
| title_sort | roles of core nosz denitrifiers in enhancing denitrification activity under long term rice straw retention |
| topic | straw retention denitrification activity nosZ gene denitrifiers paddy field |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1541202/full |
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