Stryphnodendron adstringens have a modulatory effect on inflammatory cytokines markers of in vitro activated macrophages
Aim: The purpose of this study is to conduct a comprehensive investigation into the modulatory effects of Stryphnodendron adstringens (Mart.; S. adstringens), a Brazilian wound-healing plant, on the expression of inflammatory cytokines. This will be achieved using an in vitro protocol with the comme...
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Open Exploration
2024-05-01
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| Series: | Exploration of Drug Science |
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| Online Access: | https://www.explorationpub.com/uploads/Article/A100845/100845.pdf |
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| author | Ivana Beatrice Mânica da Cruz Cibele Ferreira Teixeira Neida Luiza Pellenz Moisés Henrique Mastella Verônica Farina Azzolin Euler Esteves Ribeiro Fernanda Barbisan |
| author_facet | Ivana Beatrice Mânica da Cruz Cibele Ferreira Teixeira Neida Luiza Pellenz Moisés Henrique Mastella Verônica Farina Azzolin Euler Esteves Ribeiro Fernanda Barbisan |
| author_sort | Ivana Beatrice Mânica da Cruz |
| collection | DOAJ |
| description | Aim: The purpose of this study is to conduct a comprehensive investigation into the modulatory effects of Stryphnodendron adstringens (Mart.; S. adstringens), a Brazilian wound-healing plant, on the expression of inflammatory cytokines. This will be achieved using an in vitro protocol with the commercial macrophage cell line RAW 264.7. Methods: The macrophage inflammatory response was induced by the natural antigen phytohemagglutinin (PHA), with and without supplementation of different concentrations of S. adstringens extract. The effects on cell proliferation rate and the concentration and production of transcripts of pro-inflammatory cytokines interleukin 1β (IL-1β), IL-6, tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-γ), as well as the anti-inflammatory cytokine IL-10, were assessed using spectrophotometric, immunoassay, and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) techniques. Results: S. adstringens extract at all concentrations tested here reduced the cellular proliferation rate of activated macrophages. Extracts at concentrations of 0.49 mg/mL and 0.99 mg/mL decreased the protein and gene expression of pro-inflammatory cytokines, exhibiting the opposite effect concerning IL-10. Conclusions: The findings suggest that the wound-healing action of S. adstringens may encompass differential modulation of inflammation associated with tissue injury. |
| format | Article |
| id | doaj-art-8104d196ed1e448a976c2750d772be56 |
| institution | Kabale University |
| issn | 2836-7677 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Open Exploration |
| record_format | Article |
| series | Exploration of Drug Science |
| spelling | doaj-art-8104d196ed1e448a976c2750d772be562025-02-08T03:41:44ZengOpen ExplorationExploration of Drug Science2836-76772024-05-012325426710.37349/eds.2024.00045Stryphnodendron adstringens have a modulatory effect on inflammatory cytokines markers of in vitro activated macrophagesIvana Beatrice Mânica da Cruz0https://orcid.org/0000-0003-3008-6899Cibele Ferreira Teixeira1https://orcid.org/0000-0003-0058-3827Neida Luiza Pellenz2https://orcid.org/0000-0001-6373-9249Moisés Henrique Mastella3https://orcid.org/0000-0001-6990-6079Verônica Farina Azzolin4https://orcid.org/0000-0002-8191-5450Euler Esteves Ribeiro5https://orcid.org/0000-0002-2716-7009Fernanda Barbisan6https://orcid.org/0000-0002-2960-7047Postgraduate Program of Pharmacology, Federal University of Santa Maria, Santa Maria 97105-900, Brazil; Postgraduate Program of Gerontology, Federal University of Santa Maria, Santa Maria 97105-900, BrazilPostgraduate Program of Pharmacology, Federal University of Santa Maria, Santa Maria 97105-900, BrazilPostgraduate Program of Pharmacology, Federal University of Santa Maria, Santa Maria 97105-900, BrazilDepartment of Biophysics and Pharmacology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista, Botucatu 18618-689, BrazilPostgraduate Program of Gerontology, Federal University of Santa Maria, Santa Maria 97105-900, Brazil; Fundação Universidade Aberta da Terceira Idade, Manaus 69029-040, BrazilFundação Universidade Aberta da Terceira Idade, Manaus 69029-040, BrazilPostgraduate Program of Pharmacology, Federal University of Santa Maria, Santa Maria 97105-900, Brazil; Postgraduate Program of Gerontology, Federal University of Santa Maria, Santa Maria 97105-900, BrazilAim: The purpose of this study is to conduct a comprehensive investigation into the modulatory effects of Stryphnodendron adstringens (Mart.; S. adstringens), a Brazilian wound-healing plant, on the expression of inflammatory cytokines. This will be achieved using an in vitro protocol with the commercial macrophage cell line RAW 264.7. Methods: The macrophage inflammatory response was induced by the natural antigen phytohemagglutinin (PHA), with and without supplementation of different concentrations of S. adstringens extract. The effects on cell proliferation rate and the concentration and production of transcripts of pro-inflammatory cytokines interleukin 1β (IL-1β), IL-6, tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-γ), as well as the anti-inflammatory cytokine IL-10, were assessed using spectrophotometric, immunoassay, and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) techniques. Results: S. adstringens extract at all concentrations tested here reduced the cellular proliferation rate of activated macrophages. Extracts at concentrations of 0.49 mg/mL and 0.99 mg/mL decreased the protein and gene expression of pro-inflammatory cytokines, exhibiting the opposite effect concerning IL-10. Conclusions: The findings suggest that the wound-healing action of S. adstringens may encompass differential modulation of inflammation associated with tissue injury.https://www.explorationpub.com/uploads/Article/A100845/100845.pdfimmunomodulationphytotherapicscytokinespharmacogenomics |
| spellingShingle | Ivana Beatrice Mânica da Cruz Cibele Ferreira Teixeira Neida Luiza Pellenz Moisés Henrique Mastella Verônica Farina Azzolin Euler Esteves Ribeiro Fernanda Barbisan Stryphnodendron adstringens have a modulatory effect on inflammatory cytokines markers of in vitro activated macrophages Exploration of Drug Science immunomodulation phytotherapics cytokines pharmacogenomics |
| title | Stryphnodendron adstringens have a modulatory effect on inflammatory cytokines markers of in vitro activated macrophages |
| title_full | Stryphnodendron adstringens have a modulatory effect on inflammatory cytokines markers of in vitro activated macrophages |
| title_fullStr | Stryphnodendron adstringens have a modulatory effect on inflammatory cytokines markers of in vitro activated macrophages |
| title_full_unstemmed | Stryphnodendron adstringens have a modulatory effect on inflammatory cytokines markers of in vitro activated macrophages |
| title_short | Stryphnodendron adstringens have a modulatory effect on inflammatory cytokines markers of in vitro activated macrophages |
| title_sort | stryphnodendron adstringens have a modulatory effect on inflammatory cytokines markers of in vitro activated macrophages |
| topic | immunomodulation phytotherapics cytokines pharmacogenomics |
| url | https://www.explorationpub.com/uploads/Article/A100845/100845.pdf |
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