Protocols for the application of human embryonic stem cell-derived neurons for aging modeling and gene manipulation
Summary: In vitro models of neuronal aging and gene manipulation in human neurons (hNeurons) are valuable tools for investigating human brain aging and diseases. Here, we present a protocol for applying human embryonic stem cell (hESC)-derived neurons to model aging and the further application of sm...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725000395 |
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| author | Hui Zhang Shuhui Sun Juan Carlos Izpisua Belmonte Guang-Hui Liu Si Wang Weiqi Zhang Jing Qu |
| author_facet | Hui Zhang Shuhui Sun Juan Carlos Izpisua Belmonte Guang-Hui Liu Si Wang Weiqi Zhang Jing Qu |
| author_sort | Hui Zhang |
| collection | DOAJ |
| description | Summary: In vitro models of neuronal aging and gene manipulation in human neurons (hNeurons) are valuable tools for investigating human brain aging and diseases. Here, we present a protocol for applying human embryonic stem cell (hESC)-derived neurons to model aging and the further application of small interfering RNA (siRNA)-mediated gene silencing for functional investigations. We describe steps for neuronal differentiation and culture, siRNA transfection, and technical considerations to ensure reproducibility. Our protocol enables investigations of the molecular mechanism underlying neuronal aging and facilitates drug evaluation.For complete details on the use and execution of this protocol, please refer to Zhang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-8699fc5750424dd6887b03a0e7c0c9ec |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-8699fc5750424dd6887b03a0e7c0c9ec2025-02-11T04:35:30ZengElsevierSTAR Protocols2666-16672025-03-0161103633Protocols for the application of human embryonic stem cell-derived neurons for aging modeling and gene manipulationHui Zhang0Shuhui Sun1Juan Carlos Izpisua Belmonte2Guang-Hui Liu3Si Wang4Weiqi Zhang5Jing Qu6Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, ChinaBeijing Institute of Heart Lung and Blood Vessel Diseases, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, ChinaAltos Labs, Inc., San Diego, CA, USAKey Laboratory of Organ Regeneration and Reconstruction, State Key Laboratory of Membrane Biology, State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; Aging Biomarker Consortium (ABC), Beijing 100101, China; Corresponding authorNational Clinical Research Center for Geriatric Disorders, Aging Translational Medicine Center, Xuanwu Hospital Capital Medical University, Beijing 100053, China; Aging Biomarker Consortium (ABC), Beijing 100101, China; Corresponding authorBeijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; China National Center for Bioinformation, Beijing, China; Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China; Aging Biomarker Consortium (ABC), Beijing 100101, China; Corresponding authorBeijing Institute of Heart Lung and Blood Vessel Diseases, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China; Key Laboratory of Organ Regeneration and Reconstruction, State Key Laboratory of Membrane Biology, State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; Aging Biomarker Consortium (ABC), Beijing 100101, China; Corresponding authorSummary: In vitro models of neuronal aging and gene manipulation in human neurons (hNeurons) are valuable tools for investigating human brain aging and diseases. Here, we present a protocol for applying human embryonic stem cell (hESC)-derived neurons to model aging and the further application of small interfering RNA (siRNA)-mediated gene silencing for functional investigations. We describe steps for neuronal differentiation and culture, siRNA transfection, and technical considerations to ensure reproducibility. Our protocol enables investigations of the molecular mechanism underlying neuronal aging and facilitates drug evaluation.For complete details on the use and execution of this protocol, please refer to Zhang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725000395Cell BiologyDevelopmental biologyMolecular BiologyNeuroscienceStem CellsCell Differentiation |
| spellingShingle | Hui Zhang Shuhui Sun Juan Carlos Izpisua Belmonte Guang-Hui Liu Si Wang Weiqi Zhang Jing Qu Protocols for the application of human embryonic stem cell-derived neurons for aging modeling and gene manipulation STAR Protocols Cell Biology Developmental biology Molecular Biology Neuroscience Stem Cells Cell Differentiation |
| title | Protocols for the application of human embryonic stem cell-derived neurons for aging modeling and gene manipulation |
| title_full | Protocols for the application of human embryonic stem cell-derived neurons for aging modeling and gene manipulation |
| title_fullStr | Protocols for the application of human embryonic stem cell-derived neurons for aging modeling and gene manipulation |
| title_full_unstemmed | Protocols for the application of human embryonic stem cell-derived neurons for aging modeling and gene manipulation |
| title_short | Protocols for the application of human embryonic stem cell-derived neurons for aging modeling and gene manipulation |
| title_sort | protocols for the application of human embryonic stem cell derived neurons for aging modeling and gene manipulation |
| topic | Cell Biology Developmental biology Molecular Biology Neuroscience Stem Cells Cell Differentiation |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725000395 |
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