Coexistence of blaMIR-1 and blaNDM-1 resistance genes with a novel ST type in Enterobacter roggenkampii from a stool sample: A genome sequencing study
Objective: The Enterobacter cloacae complex, known for causing infections in hospitalised patients, displays resistance to β-lactam antibiotics due to AmpC β-lactamase expression. This study emphasises the genome sequence of Enterobacter roggenkampii, which coexists with MIR-1 and NDM-1 genes, isola...
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Elsevier
2025-01-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221371652400448X |
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| author | Qin Wang Sayyed Salman Ye Luo XiaoMei Tong |
| author_facet | Qin Wang Sayyed Salman Ye Luo XiaoMei Tong |
| author_sort | Qin Wang |
| collection | DOAJ |
| description | Objective: The Enterobacter cloacae complex, known for causing infections in hospitalised patients, displays resistance to β-lactam antibiotics due to AmpC β-lactamase expression. This study emphasises the genome sequence of Enterobacter roggenkampii, which coexists with MIR-1 and NDM-1 genes, isolated from the stool of a hospitalised patient in China. Methods: A faecal sample was diluted in Luria-Bertani broth and incubated overnight. Cultures were then spread on MacConkey agar containing meropenem and incubated for 18–24 h to select carbapenem-resistant Enterobacteriaceae. Individual colonies were isolated, and bacterial species were identified using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry and 16S rRNA gene sequencing. Genomic DNA was extracted and sequenced via Illumina and Oxford Nanopore sequencing. Results: Genomic analysis of E. roggenkampii L3897 revealed a 4 897 636 bp genome with 55.9% GC content and confirmed its classification as E. roggenkampii through average nucleotide identity-based analysis. A new sequence type distinct from ST2392, ST3014, with a unique rplB genotype was identified. Additionally, the genome harbours three antibiotic resistance genes and a newly discovered plasmid, pL3897_NDM, highlighting the need for surveillance of drug-resistant pathogens. Conclusions: The discovery of a new sequence type and the presence of an antibiotic resistance gene in E. roggenkampii L3897 underscores the need for ongoing genomic surveillance to effectively manage multidrug-resistant pathogens. |
| format | Article |
| id | doaj-art-8813ef8246a3402999da4462960cc155 |
| institution | Kabale University |
| issn | 2213-7165 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-8813ef8246a3402999da4462960cc1552025-02-08T05:00:25ZengElsevierJournal of Global Antimicrobial Resistance2213-71652025-01-01403741Coexistence of blaMIR-1 and blaNDM-1 resistance genes with a novel ST type in Enterobacter roggenkampii from a stool sample: A genome sequencing studyQin Wang0Sayyed Salman1Ye Luo2XiaoMei Tong3Center of Reproductive Medicine, Sir Run Shaw Hospital, Zhejiang University School of Medicine, Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province, Hangzhou, ChinaCollaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, ChinaCenter of Reproductive Medicine, Sir Run Shaw Hospital, Zhejiang University School of Medicine, Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province, Hangzhou, ChinaCenter of Reproductive Medicine, Sir Run Shaw Hospital, Zhejiang University School of Medicine, Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province, Hangzhou, China; Corresponding author.Objective: The Enterobacter cloacae complex, known for causing infections in hospitalised patients, displays resistance to β-lactam antibiotics due to AmpC β-lactamase expression. This study emphasises the genome sequence of Enterobacter roggenkampii, which coexists with MIR-1 and NDM-1 genes, isolated from the stool of a hospitalised patient in China. Methods: A faecal sample was diluted in Luria-Bertani broth and incubated overnight. Cultures were then spread on MacConkey agar containing meropenem and incubated for 18–24 h to select carbapenem-resistant Enterobacteriaceae. Individual colonies were isolated, and bacterial species were identified using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry and 16S rRNA gene sequencing. Genomic DNA was extracted and sequenced via Illumina and Oxford Nanopore sequencing. Results: Genomic analysis of E. roggenkampii L3897 revealed a 4 897 636 bp genome with 55.9% GC content and confirmed its classification as E. roggenkampii through average nucleotide identity-based analysis. A new sequence type distinct from ST2392, ST3014, with a unique rplB genotype was identified. Additionally, the genome harbours three antibiotic resistance genes and a newly discovered plasmid, pL3897_NDM, highlighting the need for surveillance of drug-resistant pathogens. Conclusions: The discovery of a new sequence type and the presence of an antibiotic resistance gene in E. roggenkampii L3897 underscores the need for ongoing genomic surveillance to effectively manage multidrug-resistant pathogens.http://www.sciencedirect.com/science/article/pii/S221371652400448XEnterobacter cloacae complexEnterobacter roggenkampiiNDM-1ChinaResistance |
| spellingShingle | Qin Wang Sayyed Salman Ye Luo XiaoMei Tong Coexistence of blaMIR-1 and blaNDM-1 resistance genes with a novel ST type in Enterobacter roggenkampii from a stool sample: A genome sequencing study Journal of Global Antimicrobial Resistance Enterobacter cloacae complex Enterobacter roggenkampii NDM-1 China Resistance |
| title | Coexistence of blaMIR-1 and blaNDM-1 resistance genes with a novel ST type in Enterobacter roggenkampii from a stool sample: A genome sequencing study |
| title_full | Coexistence of blaMIR-1 and blaNDM-1 resistance genes with a novel ST type in Enterobacter roggenkampii from a stool sample: A genome sequencing study |
| title_fullStr | Coexistence of blaMIR-1 and blaNDM-1 resistance genes with a novel ST type in Enterobacter roggenkampii from a stool sample: A genome sequencing study |
| title_full_unstemmed | Coexistence of blaMIR-1 and blaNDM-1 resistance genes with a novel ST type in Enterobacter roggenkampii from a stool sample: A genome sequencing study |
| title_short | Coexistence of blaMIR-1 and blaNDM-1 resistance genes with a novel ST type in Enterobacter roggenkampii from a stool sample: A genome sequencing study |
| title_sort | coexistence of blamir 1 and blandm 1 resistance genes with a novel st type in enterobacter roggenkampii from a stool sample a genome sequencing study |
| topic | Enterobacter cloacae complex Enterobacter roggenkampii NDM-1 China Resistance |
| url | http://www.sciencedirect.com/science/article/pii/S221371652400448X |
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