Phytochemical Constituents, Antimicrobial activities and Isolation of Eupatorin from the Seed Extract of <i>L. inermis</i> LINN
In low- and middle-income countries like Nigeria, traditional medicine practitioners use seeds, roots, stem barks or the whole plant to cure some ailments, hence, the need to scientifically analyze these ethnobotanical claims. The extracts of L. inermis revealed the presence of cardiac glycosides,...
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| Format: | Article |
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Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)
2020-01-01
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| Series: | Journal of Applied Sciences and Environmental Management |
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| Online Access: | https://www.Ajol.Info/index.php/jasem/article/view/192507 |
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| author | R.S. Ochu H. Ibrahim A.O. Oyewale |
| author_facet | R.S. Ochu H. Ibrahim A.O. Oyewale |
| author_sort | R.S. Ochu |
| collection | DOAJ |
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In low- and middle-income countries like Nigeria, traditional medicine practitioners use seeds, roots, stem barks or the whole plant to cure some ailments, hence, the need to scientifically analyze these ethnobotanical claims. The extracts of L. inermis revealed the presence of cardiac glycosides, saponins, flavonoids tannins, triterpenes, steroid and alkaloids. The antimicrobial screening and activities of the crude extracts were observed against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Klebsiella pneumonia with a zones of inhibition ranging from 12-26 mm which were however lower than the standard drug (Ciprofloxacin) with 28-30 mm. The MIC/MBC measurement against those microbes was found to be between 3.125 mg/ml to 50 mg/ml. The ethyl acetate extract was purified using column chromatography and eluted with ethyl acetate and ethanol solvents in various ratios. From the fractions obtained, the compound eupatorin (3’,5-dihydroxy-4’,6,7-trimethoxyflavone) was isolated and characterized using IR, 1D and 2D NMR. The eupatorin was found to show significant microbial activity against P. vulgaris, S. typhi, E. coli, S. pneumonia and S. pyogenes when compared with standard antibiotics used against these microbes. The results from this research have supported the ethnomedicinal uses of this plant in the treatment of skin infections, abdominal disorders, leprosy, rheumatalgia, edema and as a cough and cold remedy.
Keywords: Lawsonia inermis, phytochemicals, antimicrobial screening, Eupatorin
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| format | Article |
| id | doaj-art-8864d55066db486593182aaa89c2690a |
| institution | Kabale University |
| issn | 2659-1502 2659-1499 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP) |
| record_format | Article |
| series | Journal of Applied Sciences and Environmental Management |
| spelling | doaj-art-8864d55066db486593182aaa89c2690a2025-02-08T19:51:27ZengJoint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)Journal of Applied Sciences and Environmental Management2659-15022659-14992020-01-012312Phytochemical Constituents, Antimicrobial activities and Isolation of Eupatorin from the Seed Extract of <i>L. inermis</i> LINNR.S. OchuH. IbrahimA.O. Oyewale In low- and middle-income countries like Nigeria, traditional medicine practitioners use seeds, roots, stem barks or the whole plant to cure some ailments, hence, the need to scientifically analyze these ethnobotanical claims. The extracts of L. inermis revealed the presence of cardiac glycosides, saponins, flavonoids tannins, triterpenes, steroid and alkaloids. The antimicrobial screening and activities of the crude extracts were observed against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Klebsiella pneumonia with a zones of inhibition ranging from 12-26 mm which were however lower than the standard drug (Ciprofloxacin) with 28-30 mm. The MIC/MBC measurement against those microbes was found to be between 3.125 mg/ml to 50 mg/ml. The ethyl acetate extract was purified using column chromatography and eluted with ethyl acetate and ethanol solvents in various ratios. From the fractions obtained, the compound eupatorin (3’,5-dihydroxy-4’,6,7-trimethoxyflavone) was isolated and characterized using IR, 1D and 2D NMR. The eupatorin was found to show significant microbial activity against P. vulgaris, S. typhi, E. coli, S. pneumonia and S. pyogenes when compared with standard antibiotics used against these microbes. The results from this research have supported the ethnomedicinal uses of this plant in the treatment of skin infections, abdominal disorders, leprosy, rheumatalgia, edema and as a cough and cold remedy. Keywords: Lawsonia inermis, phytochemicals, antimicrobial screening, Eupatorin https://www.Ajol.Info/index.php/jasem/article/view/192507Lawsonia inermisphytochemicalsantimicrobial screeningEupatorin |
| spellingShingle | R.S. Ochu H. Ibrahim A.O. Oyewale Phytochemical Constituents, Antimicrobial activities and Isolation of Eupatorin from the Seed Extract of <i>L. inermis</i> LINN Journal of Applied Sciences and Environmental Management Lawsonia inermis phytochemicals antimicrobial screening Eupatorin |
| title | Phytochemical Constituents, Antimicrobial activities and Isolation of Eupatorin from the Seed Extract of <i>L. inermis</i> LINN |
| title_full | Phytochemical Constituents, Antimicrobial activities and Isolation of Eupatorin from the Seed Extract of <i>L. inermis</i> LINN |
| title_fullStr | Phytochemical Constituents, Antimicrobial activities and Isolation of Eupatorin from the Seed Extract of <i>L. inermis</i> LINN |
| title_full_unstemmed | Phytochemical Constituents, Antimicrobial activities and Isolation of Eupatorin from the Seed Extract of <i>L. inermis</i> LINN |
| title_short | Phytochemical Constituents, Antimicrobial activities and Isolation of Eupatorin from the Seed Extract of <i>L. inermis</i> LINN |
| title_sort | phytochemical constituents antimicrobial activities and isolation of eupatorin from the seed extract of i l inermis i linn |
| topic | Lawsonia inermis phytochemicals antimicrobial screening Eupatorin |
| url | https://www.Ajol.Info/index.php/jasem/article/view/192507 |
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