The Effect of Rosuvastatin on a murine model of deep vein thrombosis
Introduction: Rosuvastatin reduces C-reactive protein and cardiovascular mortality. After deep vein thrombosis (DVT), elevated inflammatory markers persist. We hypothesize that statins may reduce the inflammation that may be associated with the development of post thrombotic syndrome (PTS). Material...
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Elsevier
2025-03-01
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| Series: | Thrombosis Update |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666572725000033 |
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| author | Megan E. Barney Shenghao Zhou Anh Le-Cook Naly Setthavongsack Gabriel Nager Jennifer M. Loftis Randy L. Woltjer Monica Hinds Khanh P. Nguyen |
| author_facet | Megan E. Barney Shenghao Zhou Anh Le-Cook Naly Setthavongsack Gabriel Nager Jennifer M. Loftis Randy L. Woltjer Monica Hinds Khanh P. Nguyen |
| author_sort | Megan E. Barney |
| collection | DOAJ |
| description | Introduction: Rosuvastatin reduces C-reactive protein and cardiovascular mortality. After deep vein thrombosis (DVT), elevated inflammatory markers persist. We hypothesize that statins may reduce the inflammation that may be associated with the development of post thrombotic syndrome (PTS). Materials and methods: Wildtype CD1 mice were fed either regular chow or 1 mg/kg rosuvastatin diets for 1 week prior to surgically induced DVT. Thrombus weights, plasma inflammatory markers, and histology were examined on postoperative days 3 and 7. Results: Thrombus weights were equivalent in the rosuvastatin treated mice compared to control mice on day 3 (1.25 mg/g±0.61 vs 1.46 mg/g±0.61, p = 0.23) and day 7 (1.07 mg/g ± 0.39 vs 1.04 mg/g±0.32, p = 0.43). On day 3, rosuvastatin treated mice demonstrated decreased levels of monocyte chemoattractant protein-1(MCP-1) (8.20 pg/mL ± 4.07 vs 17.37 pg/mL ±3.26, p = 0.04) and tumor necrosis factor-α (TNF-α) (2.42 pg/mL ±0.42 vs 4.74 pg/mL ± 0.91, p = 0.02) in comparison to the control mice. On day 7, rosuvastatin treated mice demonstrated increased levels of interferon-γ (IFN-γ) (4.22 pg/mL ±5.02 vs 0.49 pg/mL ±0.01, p = 0.04) in comparison to the control mice. There was a significant increase in collagen deposition seen both in the thrombus (2.00 ± 0.63 vs 0.75 ± 0.46, p = 0.002) and vein wall (2.33 ± 0.82 vs 1.13 ± 0.35 p = 0.008) on day 7 in the rosuvastatin treated animals compared to the control animals. Conclusions: After DVT, rosuvastatin did not accelerate thrombus resolution nor did it affect thrombus formation. However, rosuvastatin decreases MCP-1 and TNF-α during thrombus formation and increases IFN-γ in early thrombus resolution. Additionally, rosuvastatin may promote positive remodeling within the thrombus but increases vein wall fibrosis. |
| format | Article |
| id | doaj-art-8cc2956ada7f4e4ebff0217d6c1d3592 |
| institution | Kabale University |
| issn | 2666-5727 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| series | Thrombosis Update |
| spelling | doaj-art-8cc2956ada7f4e4ebff0217d6c1d35922025-02-12T05:32:55ZengElsevierThrombosis Update2666-57272025-03-0118100200The Effect of Rosuvastatin on a murine model of deep vein thrombosisMegan E. Barney0Shenghao Zhou1Anh Le-Cook2Naly Setthavongsack3Gabriel Nager4Jennifer M. Loftis5Randy L. Woltjer6Monica Hinds7Khanh P. Nguyen8Oregon Health and Sciences University, Portland, OR, USA; Corresponding author. 3181 SW Sam Jackson Park Road, OP11, 97239, Portland, OR, USA.Pacific Northwest University-Health Sciences, Yakima, WA, USAOregon Health and Sciences University, Portland, OR, USAVA Portland Healthcare System, Portland, OR, USAOregon Health and Sciences University, Portland, OR, USAOregon Health and Sciences University, Portland, OR, USA; VA Portland Healthcare System, Portland, OR, USAOregon Health and Sciences University, Portland, OR, USAOregon Health and Sciences University, Portland, OR, USAOregon Health and Sciences University, Portland, OR, USA; VA Portland Healthcare System, Portland, OR, USAIntroduction: Rosuvastatin reduces C-reactive protein and cardiovascular mortality. After deep vein thrombosis (DVT), elevated inflammatory markers persist. We hypothesize that statins may reduce the inflammation that may be associated with the development of post thrombotic syndrome (PTS). Materials and methods: Wildtype CD1 mice were fed either regular chow or 1 mg/kg rosuvastatin diets for 1 week prior to surgically induced DVT. Thrombus weights, plasma inflammatory markers, and histology were examined on postoperative days 3 and 7. Results: Thrombus weights were equivalent in the rosuvastatin treated mice compared to control mice on day 3 (1.25 mg/g±0.61 vs 1.46 mg/g±0.61, p = 0.23) and day 7 (1.07 mg/g ± 0.39 vs 1.04 mg/g±0.32, p = 0.43). On day 3, rosuvastatin treated mice demonstrated decreased levels of monocyte chemoattractant protein-1(MCP-1) (8.20 pg/mL ± 4.07 vs 17.37 pg/mL ±3.26, p = 0.04) and tumor necrosis factor-α (TNF-α) (2.42 pg/mL ±0.42 vs 4.74 pg/mL ± 0.91, p = 0.02) in comparison to the control mice. On day 7, rosuvastatin treated mice demonstrated increased levels of interferon-γ (IFN-γ) (4.22 pg/mL ±5.02 vs 0.49 pg/mL ±0.01, p = 0.04) in comparison to the control mice. There was a significant increase in collagen deposition seen both in the thrombus (2.00 ± 0.63 vs 0.75 ± 0.46, p = 0.002) and vein wall (2.33 ± 0.82 vs 1.13 ± 0.35 p = 0.008) on day 7 in the rosuvastatin treated animals compared to the control animals. Conclusions: After DVT, rosuvastatin did not accelerate thrombus resolution nor did it affect thrombus formation. However, rosuvastatin decreases MCP-1 and TNF-α during thrombus formation and increases IFN-γ in early thrombus resolution. Additionally, rosuvastatin may promote positive remodeling within the thrombus but increases vein wall fibrosis.http://www.sciencedirect.com/science/article/pii/S2666572725000033Deep vein thrombosisThrombus resolutionRosuvastatinIVC ligationMurine modelStatin |
| spellingShingle | Megan E. Barney Shenghao Zhou Anh Le-Cook Naly Setthavongsack Gabriel Nager Jennifer M. Loftis Randy L. Woltjer Monica Hinds Khanh P. Nguyen The Effect of Rosuvastatin on a murine model of deep vein thrombosis Thrombosis Update Deep vein thrombosis Thrombus resolution Rosuvastatin IVC ligation Murine model Statin |
| title | The Effect of Rosuvastatin on a murine model of deep vein thrombosis |
| title_full | The Effect of Rosuvastatin on a murine model of deep vein thrombosis |
| title_fullStr | The Effect of Rosuvastatin on a murine model of deep vein thrombosis |
| title_full_unstemmed | The Effect of Rosuvastatin on a murine model of deep vein thrombosis |
| title_short | The Effect of Rosuvastatin on a murine model of deep vein thrombosis |
| title_sort | effect of rosuvastatin on a murine model of deep vein thrombosis |
| topic | Deep vein thrombosis Thrombus resolution Rosuvastatin IVC ligation Murine model Statin |
| url | http://www.sciencedirect.com/science/article/pii/S2666572725000033 |
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