Establishment of an animal model for monkeypox virus infection in dormice
Abstract This study aims to establish an animal model of monkeypox virus (MPXV) infection in dormice through intranasal inoculation. Male dormice aged 4–5 months were selected as experimental subjects and administered different titers of MPXV (103.5 PFU, 104.5 PFU, and 105.5 PFU, respectively) via n...
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Nature Portfolio
2025-02-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-88725-7 |
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| author | Gaojie Song Lingling Cheng Jun Liu Yu Zhou Cuiling Zhang Yuping Zong |
| author_facet | Gaojie Song Lingling Cheng Jun Liu Yu Zhou Cuiling Zhang Yuping Zong |
| author_sort | Gaojie Song |
| collection | DOAJ |
| description | Abstract This study aims to establish an animal model of monkeypox virus (MPXV) infection in dormice through intranasal inoculation. Male dormice aged 4–5 months were selected as experimental subjects and administered different titers of MPXV (103.5 PFU, 104.5 PFU, and 105.5 PFU, respectively) via nasal instillation. Within 14 days post-infection, clinical indicators such as survival rate, body weight changes, respiratory status, and mental state were continuously monitored. Additionally, tissue samples from the lungs, liver, spleen, and trachea of dormice from each group were collected on the 5th and 10th days for virus titer detection, and histopathological analysis was performed on lung samples collected on the 5th and 10th days. Dormice infected with MPXV exhibited typical symptoms such as appetite loss, continuous body weight reduction, aggravated respiratory difficulties, accompanied by lethargy, chills, and other clinical manifestations similar to human monkeypox infection. Virological tests further confirmed the distribution of MPXV in multiple vital organs of dormice, including the lungs, liver, spleen, and trachea, with particularly significant pathological damage observed in lung tissue. An MPXV infection model in dormice was successfully established through intranasal inoculation with a titer of 105.5 PFU MPXV, which can be used for studying the infection mechanism and pharmacology of MPXV. |
| format | Article |
| id | doaj-art-8ff287445d834462abe6894eeea973a7 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-8ff287445d834462abe6894eeea973a72025-02-09T12:31:43ZengNature PortfolioScientific Reports2045-23222025-02-011511810.1038/s41598-025-88725-7Establishment of an animal model for monkeypox virus infection in dormiceGaojie Song0Lingling Cheng1Jun Liu2Yu Zhou3Cuiling Zhang4Yuping Zong5Jiangxi Provincial Key Laboratory of Cell Precision Therapy, School of Basic Medical Sciences, Jiujiang UniversityJiangxi Provincial Key Laboratory of Cell Precision Therapy, School of Basic Medical Sciences, Jiujiang UniversityJiangxi Provincial Key Laboratory of Cell Precision Therapy, School of Basic Medical Sciences, Jiujiang UniversityDepartment of Disease Prevention and Control, Navy 971 HospitalChangchun Veterinary Research Institute, Chinese Academy of Agricultural SciencesCadre Ward, 964 Hospital of Joint Logistics Support ForceAbstract This study aims to establish an animal model of monkeypox virus (MPXV) infection in dormice through intranasal inoculation. Male dormice aged 4–5 months were selected as experimental subjects and administered different titers of MPXV (103.5 PFU, 104.5 PFU, and 105.5 PFU, respectively) via nasal instillation. Within 14 days post-infection, clinical indicators such as survival rate, body weight changes, respiratory status, and mental state were continuously monitored. Additionally, tissue samples from the lungs, liver, spleen, and trachea of dormice from each group were collected on the 5th and 10th days for virus titer detection, and histopathological analysis was performed on lung samples collected on the 5th and 10th days. Dormice infected with MPXV exhibited typical symptoms such as appetite loss, continuous body weight reduction, aggravated respiratory difficulties, accompanied by lethargy, chills, and other clinical manifestations similar to human monkeypox infection. Virological tests further confirmed the distribution of MPXV in multiple vital organs of dormice, including the lungs, liver, spleen, and trachea, with particularly significant pathological damage observed in lung tissue. An MPXV infection model in dormice was successfully established through intranasal inoculation with a titer of 105.5 PFU MPXV, which can be used for studying the infection mechanism and pharmacology of MPXV.https://doi.org/10.1038/s41598-025-88725-7DormiceMPXVAnimal modelPathological changes |
| spellingShingle | Gaojie Song Lingling Cheng Jun Liu Yu Zhou Cuiling Zhang Yuping Zong Establishment of an animal model for monkeypox virus infection in dormice Scientific Reports Dormice MPXV Animal model Pathological changes |
| title | Establishment of an animal model for monkeypox virus infection in dormice |
| title_full | Establishment of an animal model for monkeypox virus infection in dormice |
| title_fullStr | Establishment of an animal model for monkeypox virus infection in dormice |
| title_full_unstemmed | Establishment of an animal model for monkeypox virus infection in dormice |
| title_short | Establishment of an animal model for monkeypox virus infection in dormice |
| title_sort | establishment of an animal model for monkeypox virus infection in dormice |
| topic | Dormice MPXV Animal model Pathological changes |
| url | https://doi.org/10.1038/s41598-025-88725-7 |
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