Post sleeve gastrectomy-enriched gut commensal Clostridia promotes secondary bile acid increase and weight loss
The gut microbiome is altered after bariatric surgery and is associated with weight loss. However, the commensal bacteria involved and the underlying mechanism remain to be determined. We performed shotgun metagenomic sequencing in obese subjects before and longitudinally after sleeve gastrectomy (S...
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| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Gut Microbes |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2025.2462261 |
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| author | Shaoqian Zhao Huibin Lin Wen Li Xiaoqiang Xu Qihan Wu Zhifeng Wang Juan Shi Yufei Chen Lingxia Ye Liuqing Xi Lijia Chen Mingyang Yuan Junlei Su Aibo Gao Jiabin Jin Xiayang Ying Xiaolin Wang Yaorui Ye Yingkai Sun Yifei Zhang Xiaxing Deng Baiyong Shen Weiqiong Gu Guang Ning Weiqing Wang Jie Hong Jiqiu Wang Ruixin Liu |
| author_facet | Shaoqian Zhao Huibin Lin Wen Li Xiaoqiang Xu Qihan Wu Zhifeng Wang Juan Shi Yufei Chen Lingxia Ye Liuqing Xi Lijia Chen Mingyang Yuan Junlei Su Aibo Gao Jiabin Jin Xiayang Ying Xiaolin Wang Yaorui Ye Yingkai Sun Yifei Zhang Xiaxing Deng Baiyong Shen Weiqiong Gu Guang Ning Weiqing Wang Jie Hong Jiqiu Wang Ruixin Liu |
| author_sort | Shaoqian Zhao |
| collection | DOAJ |
| description | The gut microbiome is altered after bariatric surgery and is associated with weight loss. However, the commensal bacteria involved and the underlying mechanism remain to be determined. We performed shotgun metagenomic sequencing in obese subjects before and longitudinally after sleeve gastrectomy (SG), and found a significant enrichment in microbial species in Clostridia and bile acid metabolizing genes after SG treatment. Bile acid profiling further revealed decreased primary bile acids (PBAs) and increased conjugated secondary bile acids (C-SBAs) after SG. Specifically, glycodeoxycholic acid (GDCA) and taurodeoxycholic acid (TDCA) were increased at different follow-ups after SG, and were associated with the increased abundance of Clostridia and body weight reduction. Fecal microbiome transplantation with post-SG feces increased SBA levels, and alleviated body weight gain in the recipient mice. Furthermore, both Clostridia-enriched spore-forming bacteria and GDCA supplementation increased the expression of genes responsible for lipolysis and fatty acid oxidation in adipose tissue and reduced adiposity via Takeda G-protein-coupled receptor 5 (TGR5) signaling. Our findings reveal post-SG gut microbiome and C-SBAs as contributory to SG-induced weight loss, in part via TGR5 signaling, and suggest SBA-producing gut microbes as a potential therapeutic target for obesity intervention. |
| format | Article |
| id | doaj-art-919b443ee0e54a56a319219b968058d8 |
| institution | Kabale University |
| issn | 1949-0976 1949-0984 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj-art-919b443ee0e54a56a319219b968058d82025-02-07T03:12:52ZengTaylor & Francis GroupGut Microbes1949-09761949-09842025-12-0117110.1080/19490976.2025.2462261Post sleeve gastrectomy-enriched gut commensal Clostridia promotes secondary bile acid increase and weight lossShaoqian Zhao0Huibin Lin1Wen Li2Xiaoqiang Xu3Qihan Wu4Zhifeng Wang5Juan Shi6Yufei Chen7Lingxia Ye8Liuqing Xi9Lijia Chen10Mingyang Yuan11Junlei Su12Aibo Gao13Jiabin Jin14Xiayang Ying15Xiaolin Wang16Yaorui Ye17Yingkai Sun18Yifei Zhang19Xiaxing Deng20Baiyong Shen21Weiqiong Gu22Guang Ning23Weiqing Wang24Jie Hong25Jiqiu Wang26Ruixin Liu27Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China01life Institute, Shenzhen, Guangdong, ChinaDepartment of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China01life Institute, Shenzhen, Guangdong, ChinaDepartment of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaPancreatic Disease Center, Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaPancreatic Disease Center, Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaCAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, ChinaCAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, ChinaDepartment of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaPancreatic Disease Center, Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaPancreatic Disease Center, Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaThe gut microbiome is altered after bariatric surgery and is associated with weight loss. However, the commensal bacteria involved and the underlying mechanism remain to be determined. We performed shotgun metagenomic sequencing in obese subjects before and longitudinally after sleeve gastrectomy (SG), and found a significant enrichment in microbial species in Clostridia and bile acid metabolizing genes after SG treatment. Bile acid profiling further revealed decreased primary bile acids (PBAs) and increased conjugated secondary bile acids (C-SBAs) after SG. Specifically, glycodeoxycholic acid (GDCA) and taurodeoxycholic acid (TDCA) were increased at different follow-ups after SG, and were associated with the increased abundance of Clostridia and body weight reduction. Fecal microbiome transplantation with post-SG feces increased SBA levels, and alleviated body weight gain in the recipient mice. Furthermore, both Clostridia-enriched spore-forming bacteria and GDCA supplementation increased the expression of genes responsible for lipolysis and fatty acid oxidation in adipose tissue and reduced adiposity via Takeda G-protein-coupled receptor 5 (TGR5) signaling. Our findings reveal post-SG gut microbiome and C-SBAs as contributory to SG-induced weight loss, in part via TGR5 signaling, and suggest SBA-producing gut microbes as a potential therapeutic target for obesity intervention.https://www.tandfonline.com/doi/10.1080/19490976.2025.2462261Obesitysleeve gastrectomyClostridiabile acidsTGR5lipolysis |
| spellingShingle | Shaoqian Zhao Huibin Lin Wen Li Xiaoqiang Xu Qihan Wu Zhifeng Wang Juan Shi Yufei Chen Lingxia Ye Liuqing Xi Lijia Chen Mingyang Yuan Junlei Su Aibo Gao Jiabin Jin Xiayang Ying Xiaolin Wang Yaorui Ye Yingkai Sun Yifei Zhang Xiaxing Deng Baiyong Shen Weiqiong Gu Guang Ning Weiqing Wang Jie Hong Jiqiu Wang Ruixin Liu Post sleeve gastrectomy-enriched gut commensal Clostridia promotes secondary bile acid increase and weight loss Gut Microbes Obesity sleeve gastrectomy Clostridia bile acids TGR5 lipolysis |
| title | Post sleeve gastrectomy-enriched gut commensal Clostridia promotes secondary bile acid increase and weight loss |
| title_full | Post sleeve gastrectomy-enriched gut commensal Clostridia promotes secondary bile acid increase and weight loss |
| title_fullStr | Post sleeve gastrectomy-enriched gut commensal Clostridia promotes secondary bile acid increase and weight loss |
| title_full_unstemmed | Post sleeve gastrectomy-enriched gut commensal Clostridia promotes secondary bile acid increase and weight loss |
| title_short | Post sleeve gastrectomy-enriched gut commensal Clostridia promotes secondary bile acid increase and weight loss |
| title_sort | post sleeve gastrectomy enriched gut commensal clostridia promotes secondary bile acid increase and weight loss |
| topic | Obesity sleeve gastrectomy Clostridia bile acids TGR5 lipolysis |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2025.2462261 |
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