A meta-analysis of the effect of protein synthesis inhibitors on rodent fear conditioning
Systematic reviews and meta-analyses have been increasingly recognized for their potential value in pre-clinical research, but their applications have not been extensively explored in behavioral neuroscience. In this work, we studied protein synthesis inhibition, a classic intervention used to disru...
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2023-11-01
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| Online Access: | https://peercommunityjournal.org/articles/10.24072/pcjournal.339/ |
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| author | Carneiro, Clarissa F. D. Amorim, Felippe E. Amaral, Olavo B. |
| author_facet | Carneiro, Clarissa F. D. Amorim, Felippe E. Amaral, Olavo B. |
| author_sort | Carneiro, Clarissa F. D. |
| collection | DOAJ |
| description | Systematic reviews and meta-analyses have been increasingly recognized for their potential value in pre-clinical research, but their applications have not been extensively explored in behavioral neuroscience. In this work, we studied protein synthesis inhibition, a classic intervention used to disrupt fear learning, reconsolidation, and extinction in rodents, to explore how meta-analyses can identify potential moderators of its effect. We initially performed separate meta-analyses for different injection sites and target sessions to evaluate the effect of the intervention in various scenarios. Heterogeneity was further investigated by multilevel meta-regression models aggregating various sites, with article or research group as additional levels. We detected robust effects of protein synthesis inhibitors on training and reconsolidation, but not on extinction, possibly due to the lower number of studies on the latter. Our analyses identified some well-established moderators, such as intervention timing and reexposure duration. However, other factors proposed as boundary conditions for reconsolidation, such as memory age and training strength, were not associated with effect size. While our results point to the value of meta-analyses in consolidating findings from the literature, we believe that associations suggested by data synthesis should ideally be verified by well-powered, rigorous confirmatory experiments.
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| format | Article |
| id | doaj-art-92495dad9dd243af884ef10f3a63361a |
| institution | Kabale University |
| issn | 2804-3871 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Peer Community In |
| record_format | Article |
| series | Peer Community Journal |
| spelling | doaj-art-92495dad9dd243af884ef10f3a63361a2025-02-07T10:16:48ZengPeer Community InPeer Community Journal2804-38712023-11-01310.24072/pcjournal.33910.24072/pcjournal.339A meta-analysis of the effect of protein synthesis inhibitors on rodent fear conditioning Carneiro, Clarissa F. D.0https://orcid.org/0000-0001-8127-0034Amorim, Felippe E.1https://orcid.org/0000-0002-1922-8211Amaral, Olavo B.2https://orcid.org/0000-0002-4299-8978Institute of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil; Berlin Institute of Health (BIH) at Charité, BIH QUEST Center for Responsible Research, Berlin, GermanyInstitute of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilInstitute of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilSystematic reviews and meta-analyses have been increasingly recognized for their potential value in pre-clinical research, but their applications have not been extensively explored in behavioral neuroscience. In this work, we studied protein synthesis inhibition, a classic intervention used to disrupt fear learning, reconsolidation, and extinction in rodents, to explore how meta-analyses can identify potential moderators of its effect. We initially performed separate meta-analyses for different injection sites and target sessions to evaluate the effect of the intervention in various scenarios. Heterogeneity was further investigated by multilevel meta-regression models aggregating various sites, with article or research group as additional levels. We detected robust effects of protein synthesis inhibitors on training and reconsolidation, but not on extinction, possibly due to the lower number of studies on the latter. Our analyses identified some well-established moderators, such as intervention timing and reexposure duration. However, other factors proposed as boundary conditions for reconsolidation, such as memory age and training strength, were not associated with effect size. While our results point to the value of meta-analyses in consolidating findings from the literature, we believe that associations suggested by data synthesis should ideally be verified by well-powered, rigorous confirmatory experiments. https://peercommunityjournal.org/articles/10.24072/pcjournal.339/fear conditioning; preclinical meta-analysis; reproducibility; |
| spellingShingle | Carneiro, Clarissa F. D. Amorim, Felippe E. Amaral, Olavo B. A meta-analysis of the effect of protein synthesis inhibitors on rodent fear conditioning Peer Community Journal fear conditioning; preclinical meta-analysis; reproducibility; |
| title | A meta-analysis of the effect of protein synthesis inhibitors on rodent fear conditioning
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| title_full | A meta-analysis of the effect of protein synthesis inhibitors on rodent fear conditioning
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| title_fullStr | A meta-analysis of the effect of protein synthesis inhibitors on rodent fear conditioning
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| title_full_unstemmed | A meta-analysis of the effect of protein synthesis inhibitors on rodent fear conditioning
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| title_short | A meta-analysis of the effect of protein synthesis inhibitors on rodent fear conditioning
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| title_sort | meta analysis of the effect of protein synthesis inhibitors on rodent fear conditioning |
| topic | fear conditioning; preclinical meta-analysis; reproducibility; |
| url | https://peercommunityjournal.org/articles/10.24072/pcjournal.339/ |
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