Seed endophytes of malting barley from different locations are shaped differently and are associated with malt quality traits
Abstract Maximizing microbial functions for improving crop performance requires better understanding of the important drivers of plant-associated microbiomes. However, it remains unclear the forces that shapes microbial structure and assembly, and how plant seed-microbiome interactions impact grain...
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2025-02-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-025-06089-6 |
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| author | Oyeyemi Ajayi Ramamurthy Mahalingam |
| author_facet | Oyeyemi Ajayi Ramamurthy Mahalingam |
| author_sort | Oyeyemi Ajayi |
| collection | DOAJ |
| description | Abstract Maximizing microbial functions for improving crop performance requires better understanding of the important drivers of plant-associated microbiomes. However, it remains unclear the forces that shapes microbial structure and assembly, and how plant seed-microbiome interactions impact grain quality. In this work, we characterized the seed endophytic microbial communities of malting barley from different geographical locations and investigated associations between microbial (bacterial and fungal) species diversity and malt quality traits. Host genotype, location, and interactions (genotype x location) significantly impacted the seed endophytic microbial communities. Taxonomic composition analysis identified the most abundant genera for bacterial and fungal communities to be Bacillus (belonging to phylum Firmicutes) and Blumeria (belonging to phylum Ascomycota), respectively. We observed that a greater proportion of bacterial amplicon sequence variants (bacterial ASVs) were shared across genotypes and across locations while the greater proportion of the fungal ASVs were unique to each genotype and location. Association analysis showed a significant negative correlation between bacterial alpha diversity indices (Faith PD and Shannon indices) and malt quality traits for barley protein (BP), free amino nitrogen (FAN), diastatic power (DP) and alpha amylase (AA), while fungal alpha diversity (Shannon and Simpson) showed significant negative relationship with β-D-glucan content. In addition, some bacterial and fungal genera were significantly associated with malt extract (ME) -a key trait for maltsters and brewers. We conclude that barley genotype, location, and their interactions shape the seed endophytic microbiome and is key to microbiome manipulation and management during barley production and/or malting. |
| format | Article |
| id | doaj-art-92984436c2a24e3391d79c13d7b660a9 |
| institution | Kabale University |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-92984436c2a24e3391d79c13d7b660a92025-02-09T12:28:01ZengBMCBMC Plant Biology1471-22292025-02-0125111910.1186/s12870-025-06089-6Seed endophytes of malting barley from different locations are shaped differently and are associated with malt quality traitsOyeyemi Ajayi0Ramamurthy Mahalingam1USDA-ARS, Cereal Crops Research UnitUSDA-ARS, Cereal Crops Research UnitAbstract Maximizing microbial functions for improving crop performance requires better understanding of the important drivers of plant-associated microbiomes. However, it remains unclear the forces that shapes microbial structure and assembly, and how plant seed-microbiome interactions impact grain quality. In this work, we characterized the seed endophytic microbial communities of malting barley from different geographical locations and investigated associations between microbial (bacterial and fungal) species diversity and malt quality traits. Host genotype, location, and interactions (genotype x location) significantly impacted the seed endophytic microbial communities. Taxonomic composition analysis identified the most abundant genera for bacterial and fungal communities to be Bacillus (belonging to phylum Firmicutes) and Blumeria (belonging to phylum Ascomycota), respectively. We observed that a greater proportion of bacterial amplicon sequence variants (bacterial ASVs) were shared across genotypes and across locations while the greater proportion of the fungal ASVs were unique to each genotype and location. Association analysis showed a significant negative correlation between bacterial alpha diversity indices (Faith PD and Shannon indices) and malt quality traits for barley protein (BP), free amino nitrogen (FAN), diastatic power (DP) and alpha amylase (AA), while fungal alpha diversity (Shannon and Simpson) showed significant negative relationship with β-D-glucan content. In addition, some bacterial and fungal genera were significantly associated with malt extract (ME) -a key trait for maltsters and brewers. We conclude that barley genotype, location, and their interactions shape the seed endophytic microbiome and is key to microbiome manipulation and management during barley production and/or malting.https://doi.org/10.1186/s12870-025-06089-6BarleyEndophytesMalt qualityMicrobiomeSeeds |
| spellingShingle | Oyeyemi Ajayi Ramamurthy Mahalingam Seed endophytes of malting barley from different locations are shaped differently and are associated with malt quality traits BMC Plant Biology Barley Endophytes Malt quality Microbiome Seeds |
| title | Seed endophytes of malting barley from different locations are shaped differently and are associated with malt quality traits |
| title_full | Seed endophytes of malting barley from different locations are shaped differently and are associated with malt quality traits |
| title_fullStr | Seed endophytes of malting barley from different locations are shaped differently and are associated with malt quality traits |
| title_full_unstemmed | Seed endophytes of malting barley from different locations are shaped differently and are associated with malt quality traits |
| title_short | Seed endophytes of malting barley from different locations are shaped differently and are associated with malt quality traits |
| title_sort | seed endophytes of malting barley from different locations are shaped differently and are associated with malt quality traits |
| topic | Barley Endophytes Malt quality Microbiome Seeds |
| url | https://doi.org/10.1186/s12870-025-06089-6 |
| work_keys_str_mv | AT oyeyemiajayi seedendophytesofmaltingbarleyfromdifferentlocationsareshapeddifferentlyandareassociatedwithmaltqualitytraits AT ramamurthymahalingam seedendophytesofmaltingbarleyfromdifferentlocationsareshapeddifferentlyandareassociatedwithmaltqualitytraits |