Development and Validation of Chlamydia muridarum Mouse Models for Studying Genital Tract Infection Pathogenesis
Animal infection models play significant roles in the study of bacterial pathogenic mechanisms and host–pathogen interactions, as well as in evaluating drug and vaccine efficacies. Chlamydia trachomatis is responsible for infections in various mucosal tissues, including the eyes and urogenital, resp...
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| Format: | Article |
| Language: | English |
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Bio-protocol LLC
2025-02-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5181&type=0 |
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| author | Yihui Wang Zixuan Han Luying Wang Xin Sun Qi Tian Tianyuan Zhang |
| author_facet | Yihui Wang Zixuan Han Luying Wang Xin Sun Qi Tian Tianyuan Zhang |
| author_sort | Yihui Wang |
| collection | DOAJ |
| description | Animal infection models play significant roles in the study of bacterial pathogenic mechanisms and host–pathogen interactions, as well as in evaluating drug and vaccine efficacies. Chlamydia trachomatis is responsible for infections in various mucosal tissues, including the eyes and urogenital, respiratory, and gastrointestinal tracts. Chronic infections can result in severe consequences such as trachoma-induced blindness, ectopic pregnancy, and infertility. While intravaginal inoculation of C. muridarum mimics the natural route of sexual transmission between individuals, transcervical inoculation allows the organisms to directly infect endometrial epithelial cells without interference from host responses triggered by chlamydial contact or infection of vaginal and cervical cells. Therefore, in this study, we used mouse models to visualize pathologies in both the endometrium and oviduct following C. muridarum inoculation. |
| format | Article |
| id | doaj-art-931348bcfbfd46e5bcafd109622bdfe2 |
| institution | Kabale University |
| issn | 2331-8325 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj-art-931348bcfbfd46e5bcafd109622bdfe22025-02-07T08:16:46ZengBio-protocol LLCBio-Protocol2331-83252025-02-0115310.21769/BioProtoc.5181Development and Validation of Chlamydia muridarum Mouse Models for Studying Genital Tract Infection PathogenesisYihui Wang0Zixuan Han1Luying Wang2Xin Sun3Qi Tian4Tianyuan Zhang5Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USADepartment of Microbiology, Immunology and Molecular Genetics, University of Texas Health Science Center at San Antonio, San Antonio, TX, USAKey Laboratory of Multi-Cell Systems, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, ChinaDepartment of Obstetrics and Gynecology, 3rd Xiangya Hospital, Central South University, Changsha, Hunan, ChinaDepartment of Obstetrics and Gynecology, 3rd Xiangya Hospital, Central South University, Changsha, Hunan, ChinaDepartment of Obstetrics & Gynecology, Hunan Provincial Maternal and Child Health Care Hospital, Changsha, Hunan, ChinaShanghai Institute of Virology, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaKey Lab of Molecular Virology and Immunology, Shanghai Institute of Immunity and Infection, Chinese Academy of Sciences, Shanghai, ChinaAnimal infection models play significant roles in the study of bacterial pathogenic mechanisms and host–pathogen interactions, as well as in evaluating drug and vaccine efficacies. Chlamydia trachomatis is responsible for infections in various mucosal tissues, including the eyes and urogenital, respiratory, and gastrointestinal tracts. Chronic infections can result in severe consequences such as trachoma-induced blindness, ectopic pregnancy, and infertility. While intravaginal inoculation of C. muridarum mimics the natural route of sexual transmission between individuals, transcervical inoculation allows the organisms to directly infect endometrial epithelial cells without interference from host responses triggered by chlamydial contact or infection of vaginal and cervical cells. Therefore, in this study, we used mouse models to visualize pathologies in both the endometrium and oviduct following C. muridarum inoculation.https://bio-protocol.org/en/bpdetail?id=5181&type=0 |
| spellingShingle | Yihui Wang Zixuan Han Luying Wang Xin Sun Qi Tian Tianyuan Zhang Development and Validation of Chlamydia muridarum Mouse Models for Studying Genital Tract Infection Pathogenesis Bio-Protocol |
| title | Development and Validation of Chlamydia muridarum Mouse Models for Studying Genital Tract Infection Pathogenesis |
| title_full | Development and Validation of Chlamydia muridarum Mouse Models for Studying Genital Tract Infection Pathogenesis |
| title_fullStr | Development and Validation of Chlamydia muridarum Mouse Models for Studying Genital Tract Infection Pathogenesis |
| title_full_unstemmed | Development and Validation of Chlamydia muridarum Mouse Models for Studying Genital Tract Infection Pathogenesis |
| title_short | Development and Validation of Chlamydia muridarum Mouse Models for Studying Genital Tract Infection Pathogenesis |
| title_sort | development and validation of chlamydia muridarum mouse models for studying genital tract infection pathogenesis |
| url | https://bio-protocol.org/en/bpdetail?id=5181&type=0 |
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