The potential of local orange peel-derived eco-enzymes in producing indole acetic acid
Background: Ecoenzymes, created from the fermentation of organic citrus waste, offer a sustainable method to produce Indole Acetic Acid (IAA), a phytohormone vital for plant growth. This study investigates the potential of these ecoenzymes in promoting sustainable agriculture. Objective: This st...
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| Format: | Article |
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Indonesian Society for Biochemistry and Molecular Biology
2023-12-01
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| Series: | Acta Biochimica Indonesiana |
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| Online Access: | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/135 |
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| author | Siska Alicia Farma Nurfa Dewiza Luzik Salma Sakina Irma Leilani Eka Putri Linda Advinda Azwir Anhar |
| author_facet | Siska Alicia Farma Nurfa Dewiza Luzik Salma Sakina Irma Leilani Eka Putri Linda Advinda Azwir Anhar |
| author_sort | Siska Alicia Farma |
| collection | DOAJ |
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Background: Ecoenzymes, created from the fermentation of organic citrus waste, offer a sustainable method to produce Indole Acetic Acid (IAA), a phytohormone vital for plant growth. This study investigates the potential of these ecoenzymes in promoting sustainable agriculture.
Objective: This study aims to evaluate the capacity of ecoenzymes derived from local citrus organic waste to synthesize IAA hormones.
Methods: The ecoenzyme was extracted from fruit powders and centrifuged to separate the supernatant. One ml of ecoenzyme supernatant was then mixed with 2 ml of Salkowski reagent and incubated for 12 hours at room temperature in the dark to facilitate reaction. The presence and concentration of IAA were determined using spectrophotometry at a wavelength of 530 nm, while total protein levels were measured using the Warburg-Christian method.
Results: ecoenzymes from local citrus sources contain IAA, with the highest concentration observed in sample 7A (30.26 µg/ml). The ecoenzyme exhibited favorable characteristics, including an average degree of acidity of 3.55, and the highest total protein content was found in sample 2A (144.277 mg/mL).
Conclusion: Ecoenzymes from local orange peels successfully produce IAA, supported by fermentation-induced microbial activity and acidic conditions. This highlights their potential in sustainable agriculture.
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| format | Article |
| id | doaj-art-935a4271111a49ecba57999331c4e89a |
| institution | Kabale University |
| issn | 2654-6108 2654-3222 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Indonesian Society for Biochemistry and Molecular Biology |
| record_format | Article |
| series | Acta Biochimica Indonesiana |
| spelling | doaj-art-935a4271111a49ecba57999331c4e89a2025-02-08T03:05:53ZengIndonesian Society for Biochemistry and Molecular BiologyActa Biochimica Indonesiana2654-61082654-32222023-12-016210.32889/actabioina.135The potential of local orange peel-derived eco-enzymes in producing indole acetic acidSiska Alicia Farma0Nurfa Dewiza Luzik1Salma Sakina2Irma Leilani Eka Putri3Linda Advinda4Azwir Anhar51Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, West Sumatera, Indonesia. 2Center of Research on Recycling Organic Waste Management, Universitas Negeri PadangDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, West Sumatera, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, West Sumatera, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, West Sumatera, Indonesia. 2Center of Research on Recycling Organic Waste Management, Universitas Negeri PadangDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, West Sumatera, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, West Sumatera, Indonesia. 2Center of Research on Recycling Organic Waste Management, Universitas Negeri Padang Background: Ecoenzymes, created from the fermentation of organic citrus waste, offer a sustainable method to produce Indole Acetic Acid (IAA), a phytohormone vital for plant growth. This study investigates the potential of these ecoenzymes in promoting sustainable agriculture. Objective: This study aims to evaluate the capacity of ecoenzymes derived from local citrus organic waste to synthesize IAA hormones. Methods: The ecoenzyme was extracted from fruit powders and centrifuged to separate the supernatant. One ml of ecoenzyme supernatant was then mixed with 2 ml of Salkowski reagent and incubated for 12 hours at room temperature in the dark to facilitate reaction. The presence and concentration of IAA were determined using spectrophotometry at a wavelength of 530 nm, while total protein levels were measured using the Warburg-Christian method. Results: ecoenzymes from local citrus sources contain IAA, with the highest concentration observed in sample 7A (30.26 µg/ml). The ecoenzyme exhibited favorable characteristics, including an average degree of acidity of 3.55, and the highest total protein content was found in sample 2A (144.277 mg/mL). Conclusion: Ecoenzymes from local orange peels successfully produce IAA, supported by fermentation-induced microbial activity and acidic conditions. This highlights their potential in sustainable agriculture. https://pbbmi.org/newjurnal/index.php/actabioina/article/view/135EcoenzymeIndole Acetic AcidOrange PeelSustainable AgriculturePhytohormones |
| spellingShingle | Siska Alicia Farma Nurfa Dewiza Luzik Salma Sakina Irma Leilani Eka Putri Linda Advinda Azwir Anhar The potential of local orange peel-derived eco-enzymes in producing indole acetic acid Acta Biochimica Indonesiana Ecoenzyme Indole Acetic Acid Orange Peel Sustainable Agriculture Phytohormones |
| title | The potential of local orange peel-derived eco-enzymes in producing indole acetic acid |
| title_full | The potential of local orange peel-derived eco-enzymes in producing indole acetic acid |
| title_fullStr | The potential of local orange peel-derived eco-enzymes in producing indole acetic acid |
| title_full_unstemmed | The potential of local orange peel-derived eco-enzymes in producing indole acetic acid |
| title_short | The potential of local orange peel-derived eco-enzymes in producing indole acetic acid |
| title_sort | potential of local orange peel derived eco enzymes in producing indole acetic acid |
| topic | Ecoenzyme Indole Acetic Acid Orange Peel Sustainable Agriculture Phytohormones |
| url | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/135 |
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