Manipulation of seed coat content for increasing oil content via modulating BnaMYB52 in Brassica napus
Summary: Seed oil is synthesized in the embryo, which is surrounded by the seed coat. However, the genetic and molecular mechanisms of how seed coat development affects seed oil content (SOC) remains less studied. Through genome-wide association studies in Brassica napus accessions, we identify BnaA...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124725000518 |
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| author | Jiang Ye Xiaowei Wu Xiang Li Yuting Zhang Yuqing Li Hui Zhang Jie Chen Yuyan Xiang Yefan Xia Hu Zhao Zengdong Tan Liang Guo Xuan Yao |
| author_facet | Jiang Ye Xiaowei Wu Xiang Li Yuting Zhang Yuqing Li Hui Zhang Jie Chen Yuyan Xiang Yefan Xia Hu Zhao Zengdong Tan Liang Guo Xuan Yao |
| author_sort | Jiang Ye |
| collection | DOAJ |
| description | Summary: Seed oil is synthesized in the embryo, which is surrounded by the seed coat. However, the genetic and molecular mechanisms of how seed coat development affects seed oil content (SOC) remains less studied. Through genome-wide association studies in Brassica napus accessions, we identify BnaA09.MYB52 transcription factor as a candidate gene underlying ZS11_qSCC.A09. Knocking out BnaMYB52 results in a 7.7%–8.2% reduction in seed coat content (SCC) but a 12.3%–13.4% increase in SOC, whereas overexpression of BnaA09.MYB52 leads to the opposite effects. Intriguingly, BnaA09.MYB52 has predominant expression in seed coat during seed development. BnaA09.MYB52 activates the expression of PMEI14 and BAN. Mutant analyses indicate that the SOC is increased by 4.3%–7.7%, while seed coat thickness shows a 12.9%–22.8% reduction in the ban and pmei14 mutants. Thus, our findings identify the molecular mechanism of MYB52-BAN/PMEI14 modules regulating SCC and SOC and provide a potential target for oil content improvement in B. napus. |
| format | Article |
| id | doaj-art-950e271eb0bd459e9e5cacfc549e3d3c |
| institution | Kabale University |
| issn | 2211-1247 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj-art-950e271eb0bd459e9e5cacfc549e3d3c2025-02-12T05:30:59ZengElsevierCell Reports2211-12472025-02-01442115280Manipulation of seed coat content for increasing oil content via modulating BnaMYB52 in Brassica napusJiang Ye0Xiaowei Wu1Xiang Li2Yuting Zhang3Yuqing Li4Hui Zhang5Jie Chen6Yuyan Xiang7Yefan Xia8Hu Zhao9Zengdong Tan10Liang Guo11Xuan Yao12National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Yazhouwan National Laboratory, Sanya, Hainan 572025, ChinaNational Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaYazhouwan National Laboratory, Sanya, Hainan 572025, ChinaNational Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaYazhouwan National Laboratory, Sanya, Hainan 572025, ChinaNational Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaNational Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaYazhouwan National Laboratory, Sanya, Hainan 572025, China; Corresponding authorNational Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Yazhouwan National Laboratory, Sanya, Hainan 572025, China; Corresponding authorNational Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Yazhouwan National Laboratory, Sanya, Hainan 572025, China; Corresponding authorSummary: Seed oil is synthesized in the embryo, which is surrounded by the seed coat. However, the genetic and molecular mechanisms of how seed coat development affects seed oil content (SOC) remains less studied. Through genome-wide association studies in Brassica napus accessions, we identify BnaA09.MYB52 transcription factor as a candidate gene underlying ZS11_qSCC.A09. Knocking out BnaMYB52 results in a 7.7%–8.2% reduction in seed coat content (SCC) but a 12.3%–13.4% increase in SOC, whereas overexpression of BnaA09.MYB52 leads to the opposite effects. Intriguingly, BnaA09.MYB52 has predominant expression in seed coat during seed development. BnaA09.MYB52 activates the expression of PMEI14 and BAN. Mutant analyses indicate that the SOC is increased by 4.3%–7.7%, while seed coat thickness shows a 12.9%–22.8% reduction in the ban and pmei14 mutants. Thus, our findings identify the molecular mechanism of MYB52-BAN/PMEI14 modules regulating SCC and SOC and provide a potential target for oil content improvement in B. napus.http://www.sciencedirect.com/science/article/pii/S2211124725000518CP: Plants |
| spellingShingle | Jiang Ye Xiaowei Wu Xiang Li Yuting Zhang Yuqing Li Hui Zhang Jie Chen Yuyan Xiang Yefan Xia Hu Zhao Zengdong Tan Liang Guo Xuan Yao Manipulation of seed coat content for increasing oil content via modulating BnaMYB52 in Brassica napus Cell Reports CP: Plants |
| title | Manipulation of seed coat content for increasing oil content via modulating BnaMYB52 in Brassica napus |
| title_full | Manipulation of seed coat content for increasing oil content via modulating BnaMYB52 in Brassica napus |
| title_fullStr | Manipulation of seed coat content for increasing oil content via modulating BnaMYB52 in Brassica napus |
| title_full_unstemmed | Manipulation of seed coat content for increasing oil content via modulating BnaMYB52 in Brassica napus |
| title_short | Manipulation of seed coat content for increasing oil content via modulating BnaMYB52 in Brassica napus |
| title_sort | manipulation of seed coat content for increasing oil content via modulating bnamyb52 in brassica napus |
| topic | CP: Plants |
| url | http://www.sciencedirect.com/science/article/pii/S2211124725000518 |
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