Heterologous expression of SpsTAC2 in Arabidopsis affected branch angle and secondary vascular system development
To investigate the biological functions of Tiller Angle Control 2 (TAC2) in Salix psammophila. In this study, TAC2 was cloned from Salix psammophila, and an overexpression and subcellular localization expression vector for the SpsTAC2 gene was constructed. The SpsTAC2 gene was overexpressed in Arabi...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
|
| Series: | Plant Signaling & Behavior |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592324.2025.2450821 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823858064934043648 |
|---|---|
| author | Fangshu Niu Mengru Yuan Hongxia Zhao Zhi Pang Jie Yan RuiXie Ning Lin Shi Fengqiang Yu Dongshan Wei Rong Yang Runming Zhang Haifeng Yang |
| author_facet | Fangshu Niu Mengru Yuan Hongxia Zhao Zhi Pang Jie Yan RuiXie Ning Lin Shi Fengqiang Yu Dongshan Wei Rong Yang Runming Zhang Haifeng Yang |
| author_sort | Fangshu Niu |
| collection | DOAJ |
| description | To investigate the biological functions of Tiller Angle Control 2 (TAC2) in Salix psammophila. In this study, TAC2 was cloned from Salix psammophila, and an overexpression and subcellular localization expression vector for the SpsTAC2 gene was constructed. The SpsTAC2 gene was overexpressed in Arabidopsis and analyzed for phenotypic changes. The subcellular localization of SpsTAC2 was analyzed via Agrobacterium-mediated transient expression in onion (Allium cepa L.) epidermal cells. Phenotypic characterization of SpsTAC2 overexpressing Arabidopsis strains revealed that the branching angle of the transgenic strains was significantly greater than that of the wild type, and the anatomical structures of the stems and hypocotyls of the transgenic strains indicated that the vascular system of the transgenic strains developed more slowly than did that of the wild type. The subcellular localization of the SpsTAC2 gene revealed that the localization signals of the SpsTAC2 gene were mainly in the nucleus, and weak signals also appeared in the cell membrane, suggesting that the SpsTAC2 gene was mainly expressed mainly in the nucleus, with a small amount of expression in the cell membrane. This findings suggest that the SpsTAC2 gene influences the development of the branching angle of plants and xylem, and exerts its effects mainly in the nucleus and membrane. This study can help to characterize the regulatory effect of the TAC gene on the branching angle and explore its effect on the branching angle and vascular system development, and also help to explore the possible molecular regulatory mechanism, which can provide a theoretical basis for further elucidation of the mechanism of action of the IGT gene family. |
| format | Article |
| id | doaj-art-9836adfd39364f0f846014bc361093b0 |
| institution | Kabale University |
| issn | 1559-2316 1559-2324 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Signaling & Behavior |
| spelling | doaj-art-9836adfd39364f0f846014bc361093b02025-02-11T15:10:10ZengTaylor & Francis GroupPlant Signaling & Behavior1559-23161559-23242025-12-0120110.1080/15592324.2025.24508212450821Heterologous expression of SpsTAC2 in Arabidopsis affected branch angle and secondary vascular system developmentFangshu Niu0Mengru Yuan1Hongxia Zhao2Zhi Pang3Jie Yan4RuiXie Ning5Lin Shi6Fengqiang Yu7Dongshan Wei8Rong Yang9Runming Zhang10Haifeng Yang11Inner Mongolia Agricultural UniversitySouth China Agricultural Plant Molecular Analysis and Genetic Improvement and Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of SciencesInner Mongolia Agricultural UniversityInner Mongolia Agricultural UniversityInner Mongolia Autonomous Region Forestry and Grassland BureauInner Mongolia Autonomous Region Forestry and Grassland BureauOrdos Institute of Forestry and Grassland ScienceOrdos Forestry and Grassland Development Center in Inner MongoliaInner Mongolia Agricultural UniversityInner Mongolia Agricultural UniversityAgricultural and Animal Husbandry Technology Extension Center of Inner Mongolia Autonomous RegionInner Mongolia Agricultural UniversityTo investigate the biological functions of Tiller Angle Control 2 (TAC2) in Salix psammophila. In this study, TAC2 was cloned from Salix psammophila, and an overexpression and subcellular localization expression vector for the SpsTAC2 gene was constructed. The SpsTAC2 gene was overexpressed in Arabidopsis and analyzed for phenotypic changes. The subcellular localization of SpsTAC2 was analyzed via Agrobacterium-mediated transient expression in onion (Allium cepa L.) epidermal cells. Phenotypic characterization of SpsTAC2 overexpressing Arabidopsis strains revealed that the branching angle of the transgenic strains was significantly greater than that of the wild type, and the anatomical structures of the stems and hypocotyls of the transgenic strains indicated that the vascular system of the transgenic strains developed more slowly than did that of the wild type. The subcellular localization of the SpsTAC2 gene revealed that the localization signals of the SpsTAC2 gene were mainly in the nucleus, and weak signals also appeared in the cell membrane, suggesting that the SpsTAC2 gene was mainly expressed mainly in the nucleus, with a small amount of expression in the cell membrane. This findings suggest that the SpsTAC2 gene influences the development of the branching angle of plants and xylem, and exerts its effects mainly in the nucleus and membrane. This study can help to characterize the regulatory effect of the TAC gene on the branching angle and explore its effect on the branching angle and vascular system development, and also help to explore the possible molecular regulatory mechanism, which can provide a theoretical basis for further elucidation of the mechanism of action of the IGT gene family.http://dx.doi.org/10.1080/15592324.2025.2450821spstac2 genebranch angleplant transformationsubcellular localizationxylem development |
| spellingShingle | Fangshu Niu Mengru Yuan Hongxia Zhao Zhi Pang Jie Yan RuiXie Ning Lin Shi Fengqiang Yu Dongshan Wei Rong Yang Runming Zhang Haifeng Yang Heterologous expression of SpsTAC2 in Arabidopsis affected branch angle and secondary vascular system development Plant Signaling & Behavior spstac2 gene branch angle plant transformation subcellular localization xylem development |
| title | Heterologous expression of SpsTAC2 in Arabidopsis affected branch angle and secondary vascular system development |
| title_full | Heterologous expression of SpsTAC2 in Arabidopsis affected branch angle and secondary vascular system development |
| title_fullStr | Heterologous expression of SpsTAC2 in Arabidopsis affected branch angle and secondary vascular system development |
| title_full_unstemmed | Heterologous expression of SpsTAC2 in Arabidopsis affected branch angle and secondary vascular system development |
| title_short | Heterologous expression of SpsTAC2 in Arabidopsis affected branch angle and secondary vascular system development |
| title_sort | heterologous expression of spstac2 in arabidopsis affected branch angle and secondary vascular system development |
| topic | spstac2 gene branch angle plant transformation subcellular localization xylem development |
| url | http://dx.doi.org/10.1080/15592324.2025.2450821 |
| work_keys_str_mv | AT fangshuniu heterologousexpressionofspstac2inarabidopsisaffectedbranchangleandsecondaryvascularsystemdevelopment AT mengruyuan heterologousexpressionofspstac2inarabidopsisaffectedbranchangleandsecondaryvascularsystemdevelopment AT hongxiazhao heterologousexpressionofspstac2inarabidopsisaffectedbranchangleandsecondaryvascularsystemdevelopment AT zhipang heterologousexpressionofspstac2inarabidopsisaffectedbranchangleandsecondaryvascularsystemdevelopment AT jieyan heterologousexpressionofspstac2inarabidopsisaffectedbranchangleandsecondaryvascularsystemdevelopment AT ruixiening heterologousexpressionofspstac2inarabidopsisaffectedbranchangleandsecondaryvascularsystemdevelopment AT linshi heterologousexpressionofspstac2inarabidopsisaffectedbranchangleandsecondaryvascularsystemdevelopment AT fengqiangyu heterologousexpressionofspstac2inarabidopsisaffectedbranchangleandsecondaryvascularsystemdevelopment AT dongshanwei heterologousexpressionofspstac2inarabidopsisaffectedbranchangleandsecondaryvascularsystemdevelopment AT rongyang heterologousexpressionofspstac2inarabidopsisaffectedbranchangleandsecondaryvascularsystemdevelopment AT runmingzhang heterologousexpressionofspstac2inarabidopsisaffectedbranchangleandsecondaryvascularsystemdevelopment AT haifengyang heterologousexpressionofspstac2inarabidopsisaffectedbranchangleandsecondaryvascularsystemdevelopment |