Effect of pH and Hypo-Osmotic Stress on NaCl Electrolyte Permeation across Red Blood Cell Membrane
The study compares the effects produced by H+ concentrations in hypo-osmotically stressed NaCl solutions on permeation through RBC membrane. Iso-osmotic solutions of NaCl (160 mM) were prepared taking into account their molecular weight and osmotic concentration. Iso-osmotic solutions (25 mL) were...
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| Format: | Article |
| Language: | English |
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Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)
2019-08-01
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| Series: | Journal of Applied Sciences and Environmental Management |
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| Online Access: | https://www.Ajol.Info/index.php/jasem/article/view/188797 |
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| author | B.O. Atolaiye D.U. Edward-Ekpu T.O. Bamidele |
| author_facet | B.O. Atolaiye D.U. Edward-Ekpu T.O. Bamidele |
| author_sort | B.O. Atolaiye |
| collection | DOAJ |
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The study compares the effects produced by H+ concentrations in hypo-osmotically stressed NaCl solutions on permeation through RBC membrane. Iso-osmotic solutions of NaCl (160 mM) were prepared taking into account their molecular weight and osmotic concentration. Iso-osmotic solutions (25 mL) were diluted with 25 mL of distilled water to give half the initial concentrations (50 % hypotonic salt solutions). Hypotonic NaCl solutions of pH of 6.2, 6.8, 7.4 and 8.0 were prepared, mixed with 0.05 mL of RBC suspension and their absorbance/min for 16 min were measured using a UV/Visible spectrophotometer while the RBCs haemolyse. H+ concentration and hence pH was found to have a significant effect on ion permeability through RBC membrane. Permeation occurred faster in alkalinic solutions under hypo-osmotic stress. Permeation increased with every increase in pH and the increase is steeper at pH 7.4 – 8.0.
Keywords: Ions, Electrolyte, Permeability, Red Blood Cell, Membrane
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| format | Article |
| id | doaj-art-98db24e53f084473b0064985c8f7f317 |
| institution | Kabale University |
| issn | 2659-1502 2659-1499 |
| language | English |
| publishDate | 2019-08-01 |
| publisher | Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP) |
| record_format | Article |
| series | Journal of Applied Sciences and Environmental Management |
| spelling | doaj-art-98db24e53f084473b0064985c8f7f3172025-02-08T19:51:30ZengJoint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)Journal of Applied Sciences and Environmental Management2659-15022659-14992019-08-01237Effect of pH and Hypo-Osmotic Stress on NaCl Electrolyte Permeation across Red Blood Cell MembraneB.O. AtolaiyeD.U. Edward-EkpuT.O. Bamidele The study compares the effects produced by H+ concentrations in hypo-osmotically stressed NaCl solutions on permeation through RBC membrane. Iso-osmotic solutions of NaCl (160 mM) were prepared taking into account their molecular weight and osmotic concentration. Iso-osmotic solutions (25 mL) were diluted with 25 mL of distilled water to give half the initial concentrations (50 % hypotonic salt solutions). Hypotonic NaCl solutions of pH of 6.2, 6.8, 7.4 and 8.0 were prepared, mixed with 0.05 mL of RBC suspension and their absorbance/min for 16 min were measured using a UV/Visible spectrophotometer while the RBCs haemolyse. H+ concentration and hence pH was found to have a significant effect on ion permeability through RBC membrane. Permeation occurred faster in alkalinic solutions under hypo-osmotic stress. Permeation increased with every increase in pH and the increase is steeper at pH 7.4 – 8.0. Keywords: Ions, Electrolyte, Permeability, Red Blood Cell, Membrane https://www.Ajol.Info/index.php/jasem/article/view/188797IonsElectrolytePermeabilityRed Blood CellMembrane |
| spellingShingle | B.O. Atolaiye D.U. Edward-Ekpu T.O. Bamidele Effect of pH and Hypo-Osmotic Stress on NaCl Electrolyte Permeation across Red Blood Cell Membrane Journal of Applied Sciences and Environmental Management Ions Electrolyte Permeability Red Blood Cell Membrane |
| title | Effect of pH and Hypo-Osmotic Stress on NaCl Electrolyte Permeation across Red Blood Cell Membrane |
| title_full | Effect of pH and Hypo-Osmotic Stress on NaCl Electrolyte Permeation across Red Blood Cell Membrane |
| title_fullStr | Effect of pH and Hypo-Osmotic Stress on NaCl Electrolyte Permeation across Red Blood Cell Membrane |
| title_full_unstemmed | Effect of pH and Hypo-Osmotic Stress on NaCl Electrolyte Permeation across Red Blood Cell Membrane |
| title_short | Effect of pH and Hypo-Osmotic Stress on NaCl Electrolyte Permeation across Red Blood Cell Membrane |
| title_sort | effect of ph and hypo osmotic stress on nacl electrolyte permeation across red blood cell membrane |
| topic | Ions Electrolyte Permeability Red Blood Cell Membrane |
| url | https://www.Ajol.Info/index.php/jasem/article/view/188797 |
| work_keys_str_mv | AT boatolaiye effectofphandhypoosmoticstressonnaclelectrolytepermeationacrossredbloodcellmembrane AT duedwardekpu effectofphandhypoosmoticstressonnaclelectrolytepermeationacrossredbloodcellmembrane AT tobamidele effectofphandhypoosmoticstressonnaclelectrolytepermeationacrossredbloodcellmembrane |