Comparative analysis of dominant gut microbiota in Inflammatory Bowel Disease patients and healthy individuals: A case-control study
Background: Chronic inflammation in the gut might be linked to microbiota dysbiosis. Objective: This study aimed to investigate alterations in the gut microbiota composition of adult IBD patients compared to healthy controls. Methods: This case-control study investigated the relationship between fae...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-04-01
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| Series: | New Microbes and New Infections |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2052297525000058 |
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| author | Alireza Ahmadi Ebrahim Kouhsari Shabnam Razavi Nima Mohamadzadeh Sima Besharat Mohammad Ali Vakili Taghi Amiriani |
| author_facet | Alireza Ahmadi Ebrahim Kouhsari Shabnam Razavi Nima Mohamadzadeh Sima Besharat Mohammad Ali Vakili Taghi Amiriani |
| author_sort | Alireza Ahmadi |
| collection | DOAJ |
| description | Background: Chronic inflammation in the gut might be linked to microbiota dysbiosis. Objective: This study aimed to investigate alterations in the gut microbiota composition of adult IBD patients compared to healthy controls. Methods: This case-control study investigated the relationship between faecal microbiota composition and IBD in adults. Real-time qPCR analysis using bacterial 16S rRNA gene quantified the abundance of six key bacterial groups (Firmicutes, Lactobacillus spp., Bifidobacterium spp., Fusobacterium spp., Bacteroides fragilis, and Faecalibacterium prausnitzii) in faecal samples from 30 IBD patients (13 Crohn's disease, 17 ulcerative colitis) and 30 healthy controls. A correlation matrix was employed to assess relationships between these bacteria. Results: Real-time qPCR revealed significant differences (p-value <0.05) in the abundance of several bacterial groups between IBD patients and healthy controls. Firmicutes, Fusobacterium spp., and B. fragilis were significantly more abundant (p-value <0.05) in IBD patients compared to controls. Conversely, Lactobacillus spp. and F. prausnitzii were both significantly less abundant (p-value <0.05) in IBD patients. While some bacterial groups exhibited trends toward higher abundance in either CD or UC patients, these differences were not statistically significant (p-value >0.111). The correlation matrix analysis revealed specific co-occurrence patterns: Bacteroides showed a strong negative correlation with Prevotella, more abundant in healthy controls, suggesting a shift in dominance in IBD patients. Lactobacillus spp. and F. prausnitzii exhibited a positive correlation in healthy individuals, indicating their potential cooperative role in maintaining gut homeostasis. Conclusion: This study identified significant alterations in gut microbiota composition in adult IBD patients compared to healthy controls, with notable differences in the abundance of specific bacterial groups. These findings suggest that gut microbiota dysbiosis may play a critical role in IBD pathogenesis. The identification of specific bacterial imbalances provides a foundation for developing microbiota-based therapies, such as probiotics, prebiotics, and fecal microbiota transplantation, as potential interventions for restoring microbial balance and mitigating disease progression. Further research is needed to translate these insights into targeted therapeutic strategies and to explore their effectiveness in clinical settings. |
| format | Article |
| id | doaj-art-9bd01c303ce347d8a5b7a8b074a307ec |
| institution | Kabale University |
| issn | 2052-2975 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
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| series | New Microbes and New Infections |
| spelling | doaj-art-9bd01c303ce347d8a5b7a8b074a307ec2025-02-11T04:34:44ZengElsevierNew Microbes and New Infections2052-29752025-04-0164101567Comparative analysis of dominant gut microbiota in Inflammatory Bowel Disease patients and healthy individuals: A case-control studyAlireza Ahmadi0Ebrahim Kouhsari1Shabnam Razavi2Nima Mohamadzadeh3Sima Besharat4Mohammad Ali Vakili5Taghi Amiriani6Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran; Department of Laboratory Sciences, Faculty of Paramedicine, Golestan University of Medical Sciences, Gorgan, IranLaboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran; Department of Laboratory Sciences, Faculty of Paramedicine, Golestan University of Medical Sciences, Gorgan, IranDepartment of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, IranDepartment of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, IranGolestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, IranHealth Management and Social Development Research Center, Department of Biostatistics and Epidemiology, Faculty of Health, Golestan University of Medical Sciences, Gorgan, IranGolestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran; Corresponding author.Background: Chronic inflammation in the gut might be linked to microbiota dysbiosis. Objective: This study aimed to investigate alterations in the gut microbiota composition of adult IBD patients compared to healthy controls. Methods: This case-control study investigated the relationship between faecal microbiota composition and IBD in adults. Real-time qPCR analysis using bacterial 16S rRNA gene quantified the abundance of six key bacterial groups (Firmicutes, Lactobacillus spp., Bifidobacterium spp., Fusobacterium spp., Bacteroides fragilis, and Faecalibacterium prausnitzii) in faecal samples from 30 IBD patients (13 Crohn's disease, 17 ulcerative colitis) and 30 healthy controls. A correlation matrix was employed to assess relationships between these bacteria. Results: Real-time qPCR revealed significant differences (p-value <0.05) in the abundance of several bacterial groups between IBD patients and healthy controls. Firmicutes, Fusobacterium spp., and B. fragilis were significantly more abundant (p-value <0.05) in IBD patients compared to controls. Conversely, Lactobacillus spp. and F. prausnitzii were both significantly less abundant (p-value <0.05) in IBD patients. While some bacterial groups exhibited trends toward higher abundance in either CD or UC patients, these differences were not statistically significant (p-value >0.111). The correlation matrix analysis revealed specific co-occurrence patterns: Bacteroides showed a strong negative correlation with Prevotella, more abundant in healthy controls, suggesting a shift in dominance in IBD patients. Lactobacillus spp. and F. prausnitzii exhibited a positive correlation in healthy individuals, indicating their potential cooperative role in maintaining gut homeostasis. Conclusion: This study identified significant alterations in gut microbiota composition in adult IBD patients compared to healthy controls, with notable differences in the abundance of specific bacterial groups. These findings suggest that gut microbiota dysbiosis may play a critical role in IBD pathogenesis. The identification of specific bacterial imbalances provides a foundation for developing microbiota-based therapies, such as probiotics, prebiotics, and fecal microbiota transplantation, as potential interventions for restoring microbial balance and mitigating disease progression. Further research is needed to translate these insights into targeted therapeutic strategies and to explore their effectiveness in clinical settings.http://www.sciencedirect.com/science/article/pii/S2052297525000058Inflammatory Bowel Disease (IBD)Crohn's diseaseUlcerative ColitisBacterial compositionsReal-time qPCRFecal microbiota |
| spellingShingle | Alireza Ahmadi Ebrahim Kouhsari Shabnam Razavi Nima Mohamadzadeh Sima Besharat Mohammad Ali Vakili Taghi Amiriani Comparative analysis of dominant gut microbiota in Inflammatory Bowel Disease patients and healthy individuals: A case-control study New Microbes and New Infections Inflammatory Bowel Disease (IBD) Crohn's disease Ulcerative Colitis Bacterial compositions Real-time qPCR Fecal microbiota |
| title | Comparative analysis of dominant gut microbiota in Inflammatory Bowel Disease patients and healthy individuals: A case-control study |
| title_full | Comparative analysis of dominant gut microbiota in Inflammatory Bowel Disease patients and healthy individuals: A case-control study |
| title_fullStr | Comparative analysis of dominant gut microbiota in Inflammatory Bowel Disease patients and healthy individuals: A case-control study |
| title_full_unstemmed | Comparative analysis of dominant gut microbiota in Inflammatory Bowel Disease patients and healthy individuals: A case-control study |
| title_short | Comparative analysis of dominant gut microbiota in Inflammatory Bowel Disease patients and healthy individuals: A case-control study |
| title_sort | comparative analysis of dominant gut microbiota in inflammatory bowel disease patients and healthy individuals a case control study |
| topic | Inflammatory Bowel Disease (IBD) Crohn's disease Ulcerative Colitis Bacterial compositions Real-time qPCR Fecal microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S2052297525000058 |
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