Protocol to study the role of medial entorhinal cortex-basolateral amygdala circuit in context-induced retrieval of morphine withdrawal memory in mice
Summary: The medial entorhinal cortex (MEC) is crucial for context memory, yet its role in context-induced retrieval of morphine withdrawal memory remains to be investigated. Here, we present a protocol to evaluate the importance of projection neurons from the MEC to the basolateral amygdala (BLA) (...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672400707X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1825199438131363840 |
|---|---|
| author | Yali Fu Ting Ye Ming Chen Bin Lai Ping Zheng |
| author_facet | Yali Fu Ting Ye Ming Chen Bin Lai Ping Zheng |
| author_sort | Yali Fu |
| collection | DOAJ |
| description | Summary: The medial entorhinal cortex (MEC) is crucial for context memory, yet its role in context-induced retrieval of morphine withdrawal memory remains to be investigated. Here, we present a protocol to evaluate the importance of projection neurons from the MEC to the basolateral amygdala (BLA) (MEC-BLA neurons) in mice during context-induced retrieval of morphine withdrawal memory using a conditioned place aversion (CPA) model. We describe steps for surgical procedure and behavioral experiments. Then, we detail procedures of immunofluorescence staining and image analysis.For complete details on the use and execution of this protocol, please refer to Fu et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-9ca0640f2c014c64afce329da2218f04 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-9ca0640f2c014c64afce329da2218f042025-02-08T05:01:15ZengElsevierSTAR Protocols2666-16672025-03-0161103542Protocol to study the role of medial entorhinal cortex-basolateral amygdala circuit in context-induced retrieval of morphine withdrawal memory in miceYali Fu0Ting Ye1Ming Chen2Bin Lai3Ping Zheng4State Key Laboratory of Medical Neurobiology, Institutes of Brain Science, MOE Frontier Center for Brain Science, Department of Neurology of Zhongshan Hospital, Fudan University, Shanghai 200032, China; Eye Institute of Shandong First Medical University, State Key Laboratory Cultivation Base, Shandong Key Laboratory of Eye Diseases, School of Ophthalmology, Shandong First Medical University, Qingdao 266071, ChinaState Key Laboratory of Medical Neurobiology, Institutes of Brain Science, MOE Frontier Center for Brain Science, Department of Neurology of Zhongshan Hospital, Fudan University, Shanghai 200032, ChinaState Key Laboratory of Medical Neurobiology, Institutes of Brain Science, MOE Frontier Center for Brain Science, Department of Neurology of Zhongshan Hospital, Fudan University, Shanghai 200032, China; Corresponding authorState Key Laboratory of Medical Neurobiology, Institutes of Brain Science, MOE Frontier Center for Brain Science, Department of Neurology of Zhongshan Hospital, Fudan University, Shanghai 200032, China; Corresponding authorState Key Laboratory of Medical Neurobiology, Institutes of Brain Science, MOE Frontier Center for Brain Science, Department of Neurology of Zhongshan Hospital, Fudan University, Shanghai 200032, China; Medical College of China Three Gorges University, Yichang 443002, China; Corresponding authorSummary: The medial entorhinal cortex (MEC) is crucial for context memory, yet its role in context-induced retrieval of morphine withdrawal memory remains to be investigated. Here, we present a protocol to evaluate the importance of projection neurons from the MEC to the basolateral amygdala (BLA) (MEC-BLA neurons) in mice during context-induced retrieval of morphine withdrawal memory using a conditioned place aversion (CPA) model. We describe steps for surgical procedure and behavioral experiments. Then, we detail procedures of immunofluorescence staining and image analysis.For complete details on the use and execution of this protocol, please refer to Fu et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S266616672400707XNeuroscienceCognitive NeuroscienceBehavior |
| spellingShingle | Yali Fu Ting Ye Ming Chen Bin Lai Ping Zheng Protocol to study the role of medial entorhinal cortex-basolateral amygdala circuit in context-induced retrieval of morphine withdrawal memory in mice STAR Protocols Neuroscience Cognitive Neuroscience Behavior |
| title | Protocol to study the role of medial entorhinal cortex-basolateral amygdala circuit in context-induced retrieval of morphine withdrawal memory in mice |
| title_full | Protocol to study the role of medial entorhinal cortex-basolateral amygdala circuit in context-induced retrieval of morphine withdrawal memory in mice |
| title_fullStr | Protocol to study the role of medial entorhinal cortex-basolateral amygdala circuit in context-induced retrieval of morphine withdrawal memory in mice |
| title_full_unstemmed | Protocol to study the role of medial entorhinal cortex-basolateral amygdala circuit in context-induced retrieval of morphine withdrawal memory in mice |
| title_short | Protocol to study the role of medial entorhinal cortex-basolateral amygdala circuit in context-induced retrieval of morphine withdrawal memory in mice |
| title_sort | protocol to study the role of medial entorhinal cortex basolateral amygdala circuit in context induced retrieval of morphine withdrawal memory in mice |
| topic | Neuroscience Cognitive Neuroscience Behavior |
| url | http://www.sciencedirect.com/science/article/pii/S266616672400707X |
| work_keys_str_mv | AT yalifu protocoltostudytheroleofmedialentorhinalcortexbasolateralamygdalacircuitincontextinducedretrievalofmorphinewithdrawalmemoryinmice AT tingye protocoltostudytheroleofmedialentorhinalcortexbasolateralamygdalacircuitincontextinducedretrievalofmorphinewithdrawalmemoryinmice AT mingchen protocoltostudytheroleofmedialentorhinalcortexbasolateralamygdalacircuitincontextinducedretrievalofmorphinewithdrawalmemoryinmice AT binlai protocoltostudytheroleofmedialentorhinalcortexbasolateralamygdalacircuitincontextinducedretrievalofmorphinewithdrawalmemoryinmice AT pingzheng protocoltostudytheroleofmedialentorhinalcortexbasolateralamygdalacircuitincontextinducedretrievalofmorphinewithdrawalmemoryinmice |