Subacute toxicity of isoliquiritigenin-zein phosphatidylcholine nanoparticles on biochemical, hematological, and histopathological parameters in Sprague-Dawley rats
Aim: Isoliquiritigenin (ISL) is a natural flavonoid found in many natural plants, which exhibits numerous pharmacological properties including anti-inflammatory, antioxidant, antitumor, and antiviral activities. However, the low bioavailability and stability of ISL limit its application in clinical...
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Open Exploration
2024-04-01
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| Series: | Exploration of Drug Science |
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| author | Ke Yang Kumar Ganesan Fei Gao Chunguang Xie Jianping Chen |
| author_facet | Ke Yang Kumar Ganesan Fei Gao Chunguang Xie Jianping Chen |
| author_sort | Ke Yang |
| collection | DOAJ |
| description | Aim: Isoliquiritigenin (ISL) is a natural flavonoid found in many natural plants, which exhibits numerous pharmacological properties including anti-inflammatory, antioxidant, antitumor, and antiviral activities. However, the low bioavailability and stability of ISL limit its application in clinical practice. To overcome these limitations, ISL-zein phosphatidylcholine hybrid nanoparticles (ISL@ZLH NPs) have been developed to improve the bioavailability and stability of ISL. The present study aimed to evaluate the acute and subacute toxicity of ISL@ZLH NPs in Sprague-Dawley (SD) rats. Methods: The ISL@ZLH NPs were prepared by the solvent evaporation method. The acute toxicity was evaluated by administering a single dose of 110 mg/kg and 160 mg/kg of ISL@ZLH NPs extracted in distilled water via oral gavage in rats and mice, respectively. The subacute toxicity was evaluated by administering doses of 27.5 mg/(kg∙day), 55 mg/(kg∙day), and 110 mg/(kg∙day) of ISL@ZLH NPs for 30 days and 90 days. The biochemical, hematological, and histopathological parameters were analyzed in both studies. Results: In the acute toxicity study, no mortality or significant changes in the biochemical and hematological parameters were observed in both Kunming (KM) mice and SD rats. In the subacute toxicity study, no toxic reactions were observed in both species at all doses tested. Moreover, no significant changes in the biochemical, hematological and histopathological parameters were observed in both species. Conclusions: The results of this study suggest that ISL@ZLH NPs are safe and non-toxic in both KM mice and SD rats. The nanoparticles (NPs) did not induce any adverse effects on the biochemical, hematological, and histopathological parameters in both acute and subacute toxicity studies. These results indicate that ISL@ZLH NPs are safe for prolonged consumption. Further studies are needed to evaluate the long-term toxicity and efficacy of these NPs in vivo. |
| format | Article |
| id | doaj-art-9e17dbba3f1746ef81f3037d5145d854 |
| institution | Kabale University |
| issn | 2836-7677 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | Open Exploration |
| record_format | Article |
| series | Exploration of Drug Science |
| spelling | doaj-art-9e17dbba3f1746ef81f3037d5145d8542025-02-08T03:41:44ZengOpen ExplorationExploration of Drug Science2836-76772024-04-012223425310.37349/eds.2024.00044Subacute toxicity of isoliquiritigenin-zein phosphatidylcholine nanoparticles on biochemical, hematological, and histopathological parameters in Sprague-Dawley ratsKe Yang0Kumar Ganesan1https://orcid.org/0000-0002-3198-6066Fei Gao2Chunguang Xie3https://orcid.org/0000-0001-9120-8605Jianping Chen4https://orcid.org/0000-0001-6494-5271School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 6111137, Sichuan, ChinaSchool of Chinese Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 999077, ChinaSchool of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 6111137, Sichuan, ChinaHospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, Sichuan, ChinaSchool of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 6111137, Sichuan, China; School of Chinese Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 999077, China; Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, Sichuan, ChinaAim: Isoliquiritigenin (ISL) is a natural flavonoid found in many natural plants, which exhibits numerous pharmacological properties including anti-inflammatory, antioxidant, antitumor, and antiviral activities. However, the low bioavailability and stability of ISL limit its application in clinical practice. To overcome these limitations, ISL-zein phosphatidylcholine hybrid nanoparticles (ISL@ZLH NPs) have been developed to improve the bioavailability and stability of ISL. The present study aimed to evaluate the acute and subacute toxicity of ISL@ZLH NPs in Sprague-Dawley (SD) rats. Methods: The ISL@ZLH NPs were prepared by the solvent evaporation method. The acute toxicity was evaluated by administering a single dose of 110 mg/kg and 160 mg/kg of ISL@ZLH NPs extracted in distilled water via oral gavage in rats and mice, respectively. The subacute toxicity was evaluated by administering doses of 27.5 mg/(kg∙day), 55 mg/(kg∙day), and 110 mg/(kg∙day) of ISL@ZLH NPs for 30 days and 90 days. The biochemical, hematological, and histopathological parameters were analyzed in both studies. Results: In the acute toxicity study, no mortality or significant changes in the biochemical and hematological parameters were observed in both Kunming (KM) mice and SD rats. In the subacute toxicity study, no toxic reactions were observed in both species at all doses tested. Moreover, no significant changes in the biochemical, hematological and histopathological parameters were observed in both species. Conclusions: The results of this study suggest that ISL@ZLH NPs are safe and non-toxic in both KM mice and SD rats. The nanoparticles (NPs) did not induce any adverse effects on the biochemical, hematological, and histopathological parameters in both acute and subacute toxicity studies. These results indicate that ISL@ZLH NPs are safe for prolonged consumption. Further studies are needed to evaluate the long-term toxicity and efficacy of these NPs in vivo.https://www.explorationpub.com/uploads/Article/A100844/100844.pdfisoliquiritigenin-zein phosphatidylcholine hybrid nanoparticlesacute and subacute toxicityhematologicalbiochemicalhistopathological parameterssafe and non-toxic |
| spellingShingle | Ke Yang Kumar Ganesan Fei Gao Chunguang Xie Jianping Chen Subacute toxicity of isoliquiritigenin-zein phosphatidylcholine nanoparticles on biochemical, hematological, and histopathological parameters in Sprague-Dawley rats Exploration of Drug Science isoliquiritigenin-zein phosphatidylcholine hybrid nanoparticles acute and subacute toxicity hematological biochemical histopathological parameters safe and non-toxic |
| title | Subacute toxicity of isoliquiritigenin-zein phosphatidylcholine nanoparticles on biochemical, hematological, and histopathological parameters in Sprague-Dawley rats |
| title_full | Subacute toxicity of isoliquiritigenin-zein phosphatidylcholine nanoparticles on biochemical, hematological, and histopathological parameters in Sprague-Dawley rats |
| title_fullStr | Subacute toxicity of isoliquiritigenin-zein phosphatidylcholine nanoparticles on biochemical, hematological, and histopathological parameters in Sprague-Dawley rats |
| title_full_unstemmed | Subacute toxicity of isoliquiritigenin-zein phosphatidylcholine nanoparticles on biochemical, hematological, and histopathological parameters in Sprague-Dawley rats |
| title_short | Subacute toxicity of isoliquiritigenin-zein phosphatidylcholine nanoparticles on biochemical, hematological, and histopathological parameters in Sprague-Dawley rats |
| title_sort | subacute toxicity of isoliquiritigenin zein phosphatidylcholine nanoparticles on biochemical hematological and histopathological parameters in sprague dawley rats |
| topic | isoliquiritigenin-zein phosphatidylcholine hybrid nanoparticles acute and subacute toxicity hematological biochemical histopathological parameters safe and non-toxic |
| url | https://www.explorationpub.com/uploads/Article/A100844/100844.pdf |
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