Toxic effects of monocrotophos and glyphosate on the female reproductive organ and aromatase gene expression in climbing perch
Monocrotophos (MCP) and Glyphosate (GLY) are used as an insecticide and herbicide respectively in agricultural fields in India and other developed countries. Since agricultural fields are located close to water bodies, there is a high risk of water contamination by these chemicals with consequent re...
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KeAi Communications Co., Ltd.
2025-01-01
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| Series: | Aquaculture and Fisheries |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468550X23000746 |
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| author | Sujata Mohapatra Rajesh Kumar Jitendra Kumar Sundaray Lakshman Sahoo Mohd Ashraf Rather Sangita Dixit Vasam Manohar Kiran Rasal Sukanta K. Pradhan C.S.K. Mishra Dhananjaya Mishra SriangaTilak Patnaik |
| author_facet | Sujata Mohapatra Rajesh Kumar Jitendra Kumar Sundaray Lakshman Sahoo Mohd Ashraf Rather Sangita Dixit Vasam Manohar Kiran Rasal Sukanta K. Pradhan C.S.K. Mishra Dhananjaya Mishra SriangaTilak Patnaik |
| author_sort | Sujata Mohapatra |
| collection | DOAJ |
| description | Monocrotophos (MCP) and Glyphosate (GLY) are used as an insecticide and herbicide respectively in agricultural fields in India and other developed countries. Since agricultural fields are located close to water bodies, there is a high risk of water contamination by these chemicals with consequent residue accumulation in aquatic biota including fish. The 48 h LC50 was found to be 106 mg/L and 78 mg/L for MCP and GLY respectively. To investigate the effects of these two agrochemicals, the adult climbing perch, Anabas testudineus female fish were treated with 3 concentrations of MCP and GLY for 30 days. The MCP concentrations were 3.5×10-4% (T1), 5.3×10-4% (T2), and 10.6×10-4% (T3) and GLY concentrations were 2.6×10-4% (T1), 3.9×10-4% (T2), and 7.8×10-4% (T3). One group was kept as control. Molecular docking analysis showed that GLY interacted with brain aromatase protein at residues MET424, THR423, PRO479 with a binding energy of −10.685 and with a docking score of −4.769. In the case of MCP, the binding residues were MET424, THR423, and PHE422 ofbrain aromatase with a binding energy of −10.436 with a docking score of −2.824. Similarly, GLYinteracted with ovary aromatase at residues ASN 479, THR477, ASN 230, GLU 226, GLN 483, PRO 484, and GLU 486 with a binding energy of −10.685 and with a docking score of −5.177. In the case of MCP, the binding residues were ASP 312, THR 313, SER 481, and TRP 228 of ovary aromatase with a binding energy of −2.405 and with a docking score of - 3.372. These agrochemicals caused ovarian damage and alteration in gonadosomatic index and cytochrome P450 aromatase gene expression. |
| format | Article |
| id | doaj-art-9fa63fc93e8c4bb2abd18e7ed0c98f94 |
| institution | Kabale University |
| issn | 2468-550X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | KeAi Communications Co., Ltd. |
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| series | Aquaculture and Fisheries |
| spelling | doaj-art-9fa63fc93e8c4bb2abd18e7ed0c98f942025-02-09T05:00:50ZengKeAi Communications Co., Ltd.Aquaculture and Fisheries2468-550X2025-01-011017988Toxic effects of monocrotophos and glyphosate on the female reproductive organ and aromatase gene expression in climbing perchSujata Mohapatra0Rajesh Kumar1Jitendra Kumar Sundaray2Lakshman Sahoo3Mohd Ashraf Rather4Sangita Dixit5Vasam Manohar6Kiran Rasal7Sukanta K. Pradhan8C.S.K. Mishra9Dhananjaya Mishra10SriangaTilak Patnaik11College of Basic Science and Humanities, Odisha University of Agriculture and Technology, Bhubaneswar, 751003, IndiaICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, IndiaICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, India; Corresponding author. ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, India.ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, IndiaDivison of Fish Genetics and Biotechnology Faculty of Fisheries, Sher-e-Kashmir University of Agriculture Science and Technology, Kashmir, 190006, IndiaICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, IndiaICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, IndiaICAR-Central Institute of Fisheries Education, Mumbai, 400061, IndiaOdisha University of Agriculture and Technology, Bhubaneswar, 751003, IndiaCollege of Basic Science and Humanities, Odisha University of Agriculture and Technology, Bhubaneswar, 751003, IndiaICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, IndiaCollege of Basic Science and Humanities, Odisha University of Agriculture and Technology, Bhubaneswar, 751003, IndiaMonocrotophos (MCP) and Glyphosate (GLY) are used as an insecticide and herbicide respectively in agricultural fields in India and other developed countries. Since agricultural fields are located close to water bodies, there is a high risk of water contamination by these chemicals with consequent residue accumulation in aquatic biota including fish. The 48 h LC50 was found to be 106 mg/L and 78 mg/L for MCP and GLY respectively. To investigate the effects of these two agrochemicals, the adult climbing perch, Anabas testudineus female fish were treated with 3 concentrations of MCP and GLY for 30 days. The MCP concentrations were 3.5×10-4% (T1), 5.3×10-4% (T2), and 10.6×10-4% (T3) and GLY concentrations were 2.6×10-4% (T1), 3.9×10-4% (T2), and 7.8×10-4% (T3). One group was kept as control. Molecular docking analysis showed that GLY interacted with brain aromatase protein at residues MET424, THR423, PRO479 with a binding energy of −10.685 and with a docking score of −4.769. In the case of MCP, the binding residues were MET424, THR423, and PHE422 ofbrain aromatase with a binding energy of −10.436 with a docking score of −2.824. Similarly, GLYinteracted with ovary aromatase at residues ASN 479, THR477, ASN 230, GLU 226, GLN 483, PRO 484, and GLU 486 with a binding energy of −10.685 and with a docking score of −5.177. In the case of MCP, the binding residues were ASP 312, THR 313, SER 481, and TRP 228 of ovary aromatase with a binding energy of −2.405 and with a docking score of - 3.372. These agrochemicals caused ovarian damage and alteration in gonadosomatic index and cytochrome P450 aromatase gene expression.http://www.sciencedirect.com/science/article/pii/S2468550X23000746AromataseOvaryHistologyMolecular dockingAnabas testudineus |
| spellingShingle | Sujata Mohapatra Rajesh Kumar Jitendra Kumar Sundaray Lakshman Sahoo Mohd Ashraf Rather Sangita Dixit Vasam Manohar Kiran Rasal Sukanta K. Pradhan C.S.K. Mishra Dhananjaya Mishra SriangaTilak Patnaik Toxic effects of monocrotophos and glyphosate on the female reproductive organ and aromatase gene expression in climbing perch Aquaculture and Fisheries Aromatase Ovary Histology Molecular docking Anabas testudineus |
| title | Toxic effects of monocrotophos and glyphosate on the female reproductive organ and aromatase gene expression in climbing perch |
| title_full | Toxic effects of monocrotophos and glyphosate on the female reproductive organ and aromatase gene expression in climbing perch |
| title_fullStr | Toxic effects of monocrotophos and glyphosate on the female reproductive organ and aromatase gene expression in climbing perch |
| title_full_unstemmed | Toxic effects of monocrotophos and glyphosate on the female reproductive organ and aromatase gene expression in climbing perch |
| title_short | Toxic effects of monocrotophos and glyphosate on the female reproductive organ and aromatase gene expression in climbing perch |
| title_sort | toxic effects of monocrotophos and glyphosate on the female reproductive organ and aromatase gene expression in climbing perch |
| topic | Aromatase Ovary Histology Molecular docking Anabas testudineus |
| url | http://www.sciencedirect.com/science/article/pii/S2468550X23000746 |
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