Real-time multimodal imaging of daptomycin action on the cell wall of adherent Staphylococcus aureus
Abstract Objectives This study investigated the efficacy of daptomycin against adherent Staphylococcus aureus (S. aureus), a common colonizer of medical devices that leads to severe infections. For the first time, we evaluated the bactericidal effects of daptomycin on S. aureus immediately after adh...
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| Format: | Article |
| Language: | English |
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BMC
2025-02-01
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| Series: | BMC Research Notes |
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| Online Access: | https://doi.org/10.1186/s13104-025-07130-5 |
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| author | Alexis Canette Rym Boudjemaa Julien Deschamps Karine Steenkeste Christian Marlière Romain Briandet Marie-Pierre Fontaine-Aupart |
| author_facet | Alexis Canette Rym Boudjemaa Julien Deschamps Karine Steenkeste Christian Marlière Romain Briandet Marie-Pierre Fontaine-Aupart |
| author_sort | Alexis Canette |
| collection | DOAJ |
| description | Abstract Objectives This study investigated the efficacy of daptomycin against adherent Staphylococcus aureus (S. aureus), a common colonizer of medical devices that leads to severe infections. For the first time, we evaluated the bactericidal effects of daptomycin on S. aureus immediately after adhesion, mimicking early-stage contamination of biomaterials. Time-kill curve assay and confocal laser scanning microscopy (CLSM) were used to analyze the process dynamics. In addition, atomic force microscopy (AFM) and scanning electron microscopy (SEM) were employed to elucidate daptomycin-induced structural changes in the bacterial cell wall. Results description Daptomycin, at clinically relevant concentrations, rapidly eradicated adherent bacteria in the exponential growth phase, demonstrating an efficiency comparable to its action against planktonic cells. Prolonged exposure to the antibiotic caused marked alterations in the bacterial cell wall, including surface roughening and perforation, as revealed by multimodal imaging. However, daptomycin effectiveness diminished as biofilm formation progressed, underscoring the need for further exploration of optimized clinical strategies. |
| format | Article |
| id | doaj-art-a1520ed652b94e1aa335be45d1e0c78e |
| institution | Kabale University |
| issn | 1756-0500 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj-art-a1520ed652b94e1aa335be45d1e0c78e2025-02-09T12:09:57ZengBMCBMC Research Notes1756-05002025-02-011811610.1186/s13104-025-07130-5Real-time multimodal imaging of daptomycin action on the cell wall of adherent Staphylococcus aureusAlexis Canette0Rym Boudjemaa1Julien Deschamps2Karine Steenkeste3Christian Marlière4Romain Briandet5Marie-Pierre Fontaine-Aupart6Sorbonne Université, CNRS, Institut de Biologie Paris-Seine (IBPS), Service de microscopie électronique (IBPS-SME)Université Paris-Saclay, CNRS, Institut des Sciences Moléculaires d’OrsayUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis InstituteUniversité Paris-Saclay, CNRS, Institut des Sciences Moléculaires d’OrsayUniversité Paris-Saclay, CNRS, Institut des Sciences Moléculaires d’OrsayUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis InstituteUniversité Paris-Saclay, CNRS, Institut des Sciences Moléculaires d’OrsayAbstract Objectives This study investigated the efficacy of daptomycin against adherent Staphylococcus aureus (S. aureus), a common colonizer of medical devices that leads to severe infections. For the first time, we evaluated the bactericidal effects of daptomycin on S. aureus immediately after adhesion, mimicking early-stage contamination of biomaterials. Time-kill curve assay and confocal laser scanning microscopy (CLSM) were used to analyze the process dynamics. In addition, atomic force microscopy (AFM) and scanning electron microscopy (SEM) were employed to elucidate daptomycin-induced structural changes in the bacterial cell wall. Results description Daptomycin, at clinically relevant concentrations, rapidly eradicated adherent bacteria in the exponential growth phase, demonstrating an efficiency comparable to its action against planktonic cells. Prolonged exposure to the antibiotic caused marked alterations in the bacterial cell wall, including surface roughening and perforation, as revealed by multimodal imaging. However, daptomycin effectiveness diminished as biofilm formation progressed, underscoring the need for further exploration of optimized clinical strategies.https://doi.org/10.1186/s13104-025-07130-5DaptomycinStaphylococcus aureusAtomic force microscopyScanning electron microscopyConfocal laser scanning microscopyBacterial cell wall |
| spellingShingle | Alexis Canette Rym Boudjemaa Julien Deschamps Karine Steenkeste Christian Marlière Romain Briandet Marie-Pierre Fontaine-Aupart Real-time multimodal imaging of daptomycin action on the cell wall of adherent Staphylococcus aureus BMC Research Notes Daptomycin Staphylococcus aureus Atomic force microscopy Scanning electron microscopy Confocal laser scanning microscopy Bacterial cell wall |
| title | Real-time multimodal imaging of daptomycin action on the cell wall of adherent Staphylococcus aureus |
| title_full | Real-time multimodal imaging of daptomycin action on the cell wall of adherent Staphylococcus aureus |
| title_fullStr | Real-time multimodal imaging of daptomycin action on the cell wall of adherent Staphylococcus aureus |
| title_full_unstemmed | Real-time multimodal imaging of daptomycin action on the cell wall of adherent Staphylococcus aureus |
| title_short | Real-time multimodal imaging of daptomycin action on the cell wall of adherent Staphylococcus aureus |
| title_sort | real time multimodal imaging of daptomycin action on the cell wall of adherent staphylococcus aureus |
| topic | Daptomycin Staphylococcus aureus Atomic force microscopy Scanning electron microscopy Confocal laser scanning microscopy Bacterial cell wall |
| url | https://doi.org/10.1186/s13104-025-07130-5 |
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