Pulp stones and kidney stones-related gene: An investigation of single nucleotide polymorphisms in the gene encoding parathyroid hormone
Introduction: Pulp stones (PS), whose origins remain unclear, present a challenge for clinical practice in Endodontics. Similar to other calcifications, a relationship with parathyroid hormone is hypothesized. Purpose: The aim of this study was to investigate the association between the presence of...
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Elsevier
2025-02-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025000532 |
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| author | Prescila Mota de Oliveira Kublitski Bruna de Souza Romano Vania Gomes Moraes Manoel Damião Sousa-Neto Lívia Azeredo Alves Antunes Erika Calvano Küchler Leonardo Santos Antunes João Armando Brancher Edgard Michel-Crosato Marilisa Carneiro Leão Gabardo |
| author_facet | Prescila Mota de Oliveira Kublitski Bruna de Souza Romano Vania Gomes Moraes Manoel Damião Sousa-Neto Lívia Azeredo Alves Antunes Erika Calvano Küchler Leonardo Santos Antunes João Armando Brancher Edgard Michel-Crosato Marilisa Carneiro Leão Gabardo |
| author_sort | Prescila Mota de Oliveira Kublitski |
| collection | DOAJ |
| description | Introduction: Pulp stones (PS), whose origins remain unclear, present a challenge for clinical practice in Endodontics. Similar to other calcifications, a relationship with parathyroid hormone is hypothesized. Purpose: The aim of this study was to investigate the association between the presence of PS and kidney stones (KS) and single nucleotide polymorphisms (SNPs) in the parathyroid hormone (PTH) gene, which is related to KS. Methods: This cross-sectional study included adults of both sexes, divided into groups: with PS and without PS. PS diagnosis was based on radiographic evaluation. Saliva samples were collected from all participants, and prior history of KS was recorded. The samples were processed, and genomic DNA was used to genotype the rs694, rs6256, and rs307247 SNPs. The Hardy-Weinberg equilibrium was assessed using the Chi-square test. Genotypic and allelic profiles under additive, dominant, and recessive models were evaluated using a univariate logistic regression model and the Wald test, with analyses conducted in SPSS® version 23.0. Additionally, Fisher's exact test was used to compare the haplotype frequencies. Statistical significance was set at 5 %. Results: The study included 63 patients with PS and 54 without PS, with a mean age of 32.5 years. No statistically significant association was observed between the groups regarding the presence of KS. Allelic and genotypic analyses revealed no significant association (P > 0.05) between the presence of PS and SNPs analyzed in the groups studied. Conclusion: None of the SNPs studied in the gene encoding PTH were associated with PS or KS. |
| format | Article |
| id | doaj-art-a3d85efb097349b0b20211947a0fe031 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-a3d85efb097349b0b20211947a0fe0312025-02-07T04:47:53ZengElsevierHeliyon2405-84402025-02-01113e41673Pulp stones and kidney stones-related gene: An investigation of single nucleotide polymorphisms in the gene encoding parathyroid hormonePrescila Mota de Oliveira Kublitski0Bruna de Souza Romano1Vania Gomes Moraes2Manoel Damião Sousa-Neto3Lívia Azeredo Alves Antunes4Erika Calvano Küchler5Leonardo Santos Antunes6João Armando Brancher7Edgard Michel-Crosato8Marilisa Carneiro Leão Gabardo9School of Health Sciences, Universidade Positivo, Curitiba, BrazilSchool of Health Sciences, Universidade Positivo, Curitiba, BrazilPostgraduate Program in Dentistry, Universidade Federal Fluminense, Niterói, BrazilDepartment of Restorative Dentistry, Universidade de São Paulo, São Paulo, BrazilPostgraduate Program in Dentistry, Universidade Federal Fluminense, Nova Friburgo, BrazilSchool of Dentistry, Universidade Tuiuti do Paraná, Curitiba, Brazil and Department of Orthodontics, University Hospital Bonn, Bonn, GermanyPostgraduate Program in Dentistry, Universidade Federal Fluminense, Niterói, BrazilSchool of Health Sciences, Universidade Positivo, Curitiba, BrazilDepartment of Community Dentistry, Faculdade de Odontologia da Universidade de São Paulo, São Paulo, BrazilSchool of Health Sciences, Universidade Positivo, Curitiba, Brazil; Corresponding author. Rua Prof. Pedro Viriato Parigot de Souza, 5300, 81280-330, Curitiba, Paraná, Brazil.Introduction: Pulp stones (PS), whose origins remain unclear, present a challenge for clinical practice in Endodontics. Similar to other calcifications, a relationship with parathyroid hormone is hypothesized. Purpose: The aim of this study was to investigate the association between the presence of PS and kidney stones (KS) and single nucleotide polymorphisms (SNPs) in the parathyroid hormone (PTH) gene, which is related to KS. Methods: This cross-sectional study included adults of both sexes, divided into groups: with PS and without PS. PS diagnosis was based on radiographic evaluation. Saliva samples were collected from all participants, and prior history of KS was recorded. The samples were processed, and genomic DNA was used to genotype the rs694, rs6256, and rs307247 SNPs. The Hardy-Weinberg equilibrium was assessed using the Chi-square test. Genotypic and allelic profiles under additive, dominant, and recessive models were evaluated using a univariate logistic regression model and the Wald test, with analyses conducted in SPSS® version 23.0. Additionally, Fisher's exact test was used to compare the haplotype frequencies. Statistical significance was set at 5 %. Results: The study included 63 patients with PS and 54 without PS, with a mean age of 32.5 years. No statistically significant association was observed between the groups regarding the presence of KS. Allelic and genotypic analyses revealed no significant association (P > 0.05) between the presence of PS and SNPs analyzed in the groups studied. Conclusion: None of the SNPs studied in the gene encoding PTH were associated with PS or KS.http://www.sciencedirect.com/science/article/pii/S2405844025000532GenesParathyroid hormonePulp stonesSingle nucleotide polymorphism |
| spellingShingle | Prescila Mota de Oliveira Kublitski Bruna de Souza Romano Vania Gomes Moraes Manoel Damião Sousa-Neto Lívia Azeredo Alves Antunes Erika Calvano Küchler Leonardo Santos Antunes João Armando Brancher Edgard Michel-Crosato Marilisa Carneiro Leão Gabardo Pulp stones and kidney stones-related gene: An investigation of single nucleotide polymorphisms in the gene encoding parathyroid hormone Heliyon Genes Parathyroid hormone Pulp stones Single nucleotide polymorphism |
| title | Pulp stones and kidney stones-related gene: An investigation of single nucleotide polymorphisms in the gene encoding parathyroid hormone |
| title_full | Pulp stones and kidney stones-related gene: An investigation of single nucleotide polymorphisms in the gene encoding parathyroid hormone |
| title_fullStr | Pulp stones and kidney stones-related gene: An investigation of single nucleotide polymorphisms in the gene encoding parathyroid hormone |
| title_full_unstemmed | Pulp stones and kidney stones-related gene: An investigation of single nucleotide polymorphisms in the gene encoding parathyroid hormone |
| title_short | Pulp stones and kidney stones-related gene: An investigation of single nucleotide polymorphisms in the gene encoding parathyroid hormone |
| title_sort | pulp stones and kidney stones related gene an investigation of single nucleotide polymorphisms in the gene encoding parathyroid hormone |
| topic | Genes Parathyroid hormone Pulp stones Single nucleotide polymorphism |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025000532 |
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