High-efficiency biodegradation of crude oil and p-hydroxybenzoic acid by Acinetobacter haemolyticus JS-1: Integrated characterization and genomic analysis
Crude oil contamination is a major threat to both the environment and human health. Acinetobacter haemolyticus strain JS-1 was isolated from oil-contaminated soil in Jiangsu Oilfield, China, which exhibits exceptional biodegradation capabilities for crude oil and p-hydroxybenzoic acid (PHBA). This s...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325001162 |
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| author | Weiyi Tao Jijie Lu Junzhang Lin Mingshan Ding Weidong Wang Shuang Li |
| author_facet | Weiyi Tao Jijie Lu Junzhang Lin Mingshan Ding Weidong Wang Shuang Li |
| author_sort | Weiyi Tao |
| collection | DOAJ |
| description | Crude oil contamination is a major threat to both the environment and human health. Acinetobacter haemolyticus strain JS-1 was isolated from oil-contaminated soil in Jiangsu Oilfield, China, which exhibits exceptional biodegradation capabilities for crude oil and p-hydroxybenzoic acid (PHBA). This strain can degrade 70–80 % of crude oil at concentrations of 10–20 g/L within 15 days at 30 °C. Strain JS-1 does not produce surfactants but absorbs crude oil through cell surface hydrophobicity. RT-qPCR analysis has identified five types of alkane hydroxylase genes in strain JS-1, including two AlkB-types, one AlmA-type, one LadA-type, and a cytochrome P450 monooxygenase. These genes are likely contributed to its high degradation efficiency. Notably, the CYP153 enzyme is crucial for metabolizing hexadecane and octadecane. We further investigated the PHBA degradation pathway, revealing both the protocatechuate ortho-cleavage pathway and a potential ubiquinone biosynthesis pathway. The remarkable efficiency of strain JS-1 in crude oil biodegradation positions it as a promising candidate for mitigating crude oil pollution. Moreover, its effective PHBA degradation provides new opportunities for the application of microorganism in agricultural management. |
| format | Article |
| id | doaj-art-a46132e391ec46cca4f43d152dc9f1b2 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-a46132e391ec46cca4f43d152dc9f1b22025-02-12T05:30:20ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01290117780High-efficiency biodegradation of crude oil and p-hydroxybenzoic acid by Acinetobacter haemolyticus JS-1: Integrated characterization and genomic analysisWeiyi Tao0Jijie Lu1Junzhang Lin2Mingshan Ding3Weidong Wang4Shuang Li5College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, ChinaCollege of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, ChinaOil Production Research Institute, Shengli Oil Field Ltd. Co. SinoPEC, Dongying, ChinaOil Production Research Institute, Shengli Oil Field Ltd. Co. SinoPEC, Dongying, ChinaOil Production Research Institute, Shengli Oil Field Ltd. Co. SinoPEC, Dongying, ChinaCollege of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China; Corresponding author.Crude oil contamination is a major threat to both the environment and human health. Acinetobacter haemolyticus strain JS-1 was isolated from oil-contaminated soil in Jiangsu Oilfield, China, which exhibits exceptional biodegradation capabilities for crude oil and p-hydroxybenzoic acid (PHBA). This strain can degrade 70–80 % of crude oil at concentrations of 10–20 g/L within 15 days at 30 °C. Strain JS-1 does not produce surfactants but absorbs crude oil through cell surface hydrophobicity. RT-qPCR analysis has identified five types of alkane hydroxylase genes in strain JS-1, including two AlkB-types, one AlmA-type, one LadA-type, and a cytochrome P450 monooxygenase. These genes are likely contributed to its high degradation efficiency. Notably, the CYP153 enzyme is crucial for metabolizing hexadecane and octadecane. We further investigated the PHBA degradation pathway, revealing both the protocatechuate ortho-cleavage pathway and a potential ubiquinone biosynthesis pathway. The remarkable efficiency of strain JS-1 in crude oil biodegradation positions it as a promising candidate for mitigating crude oil pollution. Moreover, its effective PHBA degradation provides new opportunities for the application of microorganism in agricultural management.http://www.sciencedirect.com/science/article/pii/S0147651325001162Acinetobacter haemolyticusCrude oilBiodegradationp-hydroxybenzoic acidAlkane hydroxylase |
| spellingShingle | Weiyi Tao Jijie Lu Junzhang Lin Mingshan Ding Weidong Wang Shuang Li High-efficiency biodegradation of crude oil and p-hydroxybenzoic acid by Acinetobacter haemolyticus JS-1: Integrated characterization and genomic analysis Ecotoxicology and Environmental Safety Acinetobacter haemolyticus Crude oil Biodegradation p-hydroxybenzoic acid Alkane hydroxylase |
| title | High-efficiency biodegradation of crude oil and p-hydroxybenzoic acid by Acinetobacter haemolyticus JS-1: Integrated characterization and genomic analysis |
| title_full | High-efficiency biodegradation of crude oil and p-hydroxybenzoic acid by Acinetobacter haemolyticus JS-1: Integrated characterization and genomic analysis |
| title_fullStr | High-efficiency biodegradation of crude oil and p-hydroxybenzoic acid by Acinetobacter haemolyticus JS-1: Integrated characterization and genomic analysis |
| title_full_unstemmed | High-efficiency biodegradation of crude oil and p-hydroxybenzoic acid by Acinetobacter haemolyticus JS-1: Integrated characterization and genomic analysis |
| title_short | High-efficiency biodegradation of crude oil and p-hydroxybenzoic acid by Acinetobacter haemolyticus JS-1: Integrated characterization and genomic analysis |
| title_sort | high efficiency biodegradation of crude oil and p hydroxybenzoic acid by acinetobacter haemolyticus js 1 integrated characterization and genomic analysis |
| topic | Acinetobacter haemolyticus Crude oil Biodegradation p-hydroxybenzoic acid Alkane hydroxylase |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325001162 |
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