Motion-tolerant 3D volumetric multimodality microscopy imaging of human skin with subcellular resolution and extended field-of-view
Abstract The skin, the body’s largest organ, has a heterogeneous structure with various cell types and tissue layers. In vivo noninvasive 3D volumetric imaging with multi-contrast, high resolution, a large field-of-view (FOV), and no-motion artifacts is crucial for studying skin biology and diagnosi...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-02-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-025-07614-x |
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| author | Zhenguo Wu Yunxian Tian Jianhua Zhao Yimei Huang Harvey Lui Sunil Kalia Haishan Zeng |
| author_facet | Zhenguo Wu Yunxian Tian Jianhua Zhao Yimei Huang Harvey Lui Sunil Kalia Haishan Zeng |
| author_sort | Zhenguo Wu |
| collection | DOAJ |
| description | Abstract The skin, the body’s largest organ, has a heterogeneous structure with various cell types and tissue layers. In vivo noninvasive 3D volumetric imaging with multi-contrast, high resolution, a large field-of-view (FOV), and no-motion artifacts is crucial for studying skin biology and diagnosing/evaluating diseases. Traditionally high-resolution in vivo skin microscopy methods capture images in the en-face (xy) plane parallel to the skin surface but are affected by involuntary motion, particularly during large-area volumetric data acquisition using xy-z mosaicking. In this work, we developed an xz-y imaging method that acquires images in the vertical (xz) plane and extends the FOV by moving the skin laterally along the y-direction. This approach is conceived based on our observation that involuntary skin movements are mostly along the vertical direction. Combined with a unique motion correction method, it enables 3D image reconstruction with subcellular resolution and an extended FOV close to a centimeter (8 mm). A multimodality microscopy system using this method provides simultaneous reflectance confocal, two-photon excited fluorescence, and second harmonic generation imaging, enabling multi-contrast capabilities. Using this system, we captured histology-like features of normal skin, vitiligo, and melanoma, demonstrating its potential for in vivo skin biology studies, clinical diagnosis, treatment planning and monitoring. |
| format | Article |
| id | doaj-art-a4968696db6145369d902efa011f504e |
| institution | Kabale University |
| issn | 2399-3642 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj-art-a4968696db6145369d902efa011f504e2025-02-09T12:50:37ZengNature PortfolioCommunications Biology2399-36422025-02-018111510.1038/s42003-025-07614-xMotion-tolerant 3D volumetric multimodality microscopy imaging of human skin with subcellular resolution and extended field-of-viewZhenguo Wu0Yunxian Tian1Jianhua Zhao2Yimei Huang3Harvey Lui4Sunil Kalia5Haishan Zeng6Imaging Unit, Integrative Oncology Department, BC Cancer Research InstituteImaging Unit, Integrative Oncology Department, BC Cancer Research InstituteImaging Unit, Integrative Oncology Department, BC Cancer Research InstituteImaging Unit, Integrative Oncology Department, BC Cancer Research InstituteImaging Unit, Integrative Oncology Department, BC Cancer Research InstituteDepartment of Dermatology and Skin Science, Vancouver Coastal Health Research Institute & University of British ColumbiaImaging Unit, Integrative Oncology Department, BC Cancer Research InstituteAbstract The skin, the body’s largest organ, has a heterogeneous structure with various cell types and tissue layers. In vivo noninvasive 3D volumetric imaging with multi-contrast, high resolution, a large field-of-view (FOV), and no-motion artifacts is crucial for studying skin biology and diagnosing/evaluating diseases. Traditionally high-resolution in vivo skin microscopy methods capture images in the en-face (xy) plane parallel to the skin surface but are affected by involuntary motion, particularly during large-area volumetric data acquisition using xy-z mosaicking. In this work, we developed an xz-y imaging method that acquires images in the vertical (xz) plane and extends the FOV by moving the skin laterally along the y-direction. This approach is conceived based on our observation that involuntary skin movements are mostly along the vertical direction. Combined with a unique motion correction method, it enables 3D image reconstruction with subcellular resolution and an extended FOV close to a centimeter (8 mm). A multimodality microscopy system using this method provides simultaneous reflectance confocal, two-photon excited fluorescence, and second harmonic generation imaging, enabling multi-contrast capabilities. Using this system, we captured histology-like features of normal skin, vitiligo, and melanoma, demonstrating its potential for in vivo skin biology studies, clinical diagnosis, treatment planning and monitoring.https://doi.org/10.1038/s42003-025-07614-x |
| spellingShingle | Zhenguo Wu Yunxian Tian Jianhua Zhao Yimei Huang Harvey Lui Sunil Kalia Haishan Zeng Motion-tolerant 3D volumetric multimodality microscopy imaging of human skin with subcellular resolution and extended field-of-view Communications Biology |
| title | Motion-tolerant 3D volumetric multimodality microscopy imaging of human skin with subcellular resolution and extended field-of-view |
| title_full | Motion-tolerant 3D volumetric multimodality microscopy imaging of human skin with subcellular resolution and extended field-of-view |
| title_fullStr | Motion-tolerant 3D volumetric multimodality microscopy imaging of human skin with subcellular resolution and extended field-of-view |
| title_full_unstemmed | Motion-tolerant 3D volumetric multimodality microscopy imaging of human skin with subcellular resolution and extended field-of-view |
| title_short | Motion-tolerant 3D volumetric multimodality microscopy imaging of human skin with subcellular resolution and extended field-of-view |
| title_sort | motion tolerant 3d volumetric multimodality microscopy imaging of human skin with subcellular resolution and extended field of view |
| url | https://doi.org/10.1038/s42003-025-07614-x |
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