Longitudinal Survey of Astrovirus infection in different bat species in Zimbabwe: Evidence of high genetic Astrovirus diversity
Astroviruses (AstVs) have been discovered in over 80 animal species including diverse bat species and avian species. A study on Astrovirus circulation and diversity in different insectivorous and frugivorous chiropteran species roosting in trees, caves and building basements was carried out at 11 di...
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Peer Community In
2023-11-01
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| author | Chidoti, Vimbiso De Nys, Hélène Abdi, Malika Mashura, Getrude Pinarello, Valérie Chiweshe, Ngoni Matope, Gift Guerrini, Laure Pfukenyi, Davies Cappelle, Julien Mwandiringana, Ellen Missé, Dorothée Gori, Elizabeth Bourgarel, Mathieu Liégeois, Florian |
| author_facet | Chidoti, Vimbiso De Nys, Hélène Abdi, Malika Mashura, Getrude Pinarello, Valérie Chiweshe, Ngoni Matope, Gift Guerrini, Laure Pfukenyi, Davies Cappelle, Julien Mwandiringana, Ellen Missé, Dorothée Gori, Elizabeth Bourgarel, Mathieu Liégeois, Florian |
| author_sort | Chidoti, Vimbiso |
| collection | DOAJ |
| description | Astroviruses (AstVs) have been discovered in over 80 animal species including diverse bat species and avian species. A study on Astrovirus circulation and diversity in different insectivorous and frugivorous chiropteran species roosting in trees, caves and building basements was carried out at 11 different sites across Zimbabwe. Pooled and individual faecal sampling methods were used for this study, with collection dates ranging from June 2016 to July 2021. In two sites, Magweto and Chirundu, sampling was carried out at monthly intervals from August 2020 to July 2021. Astroviruses and bat mitochondrial genes were amplified using pan-AstVs and CytB /12S RNA PCR systems respectively. Phylogenetic analysis of the RdRp Astrovirus sequences revealed a high genetic diversity of astroviruses. All the bat astroviruses tested in this study clustered with the Mamastrovirus genus. Two distinct groups of amplified sequences were identified. One group was comprised of sequences isolated from Hipposideros, Rhinolophus and Eidolon helvum spp. clustered with Human Astrovirus strains: HuAstV types1-6, HuAstV-MLB1-3 and HuAstV-VA-1. The second group comprising the majority of the sequences clustered with different strains of Bat AstVs. Results from the longitudinal study at Magweto and Chirundu showed an overall AstV prevalence of 13.7% and 10.4% respectively. Peaks of AstV infection at Chirundu coincided with the period when juveniles are 4-6 months old. By combining data from our previous work on Coronaviruses, we also anaylzed co-infection of bats with Coronaviruses and Astroviruses at Magweto and Chirundu sites for which the prevalence of co-infection was 2.6% and 3.5% respectively.
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| format | Article |
| id | doaj-art-a5ad1f04756c492c86aa7479cca429f5 |
| institution | Kabale University |
| issn | 2804-3871 |
| language | English |
| publishDate | 2023-11-01 |
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| spelling | doaj-art-a5ad1f04756c492c86aa7479cca429f52025-02-07T10:16:48ZengPeer Community InPeer Community Journal2804-38712023-11-01310.24072/pcjournal.34010.24072/pcjournal.340Longitudinal Survey of Astrovirus infection in different bat species in Zimbabwe: Evidence of high genetic Astrovirus diversity Chidoti, Vimbiso0De Nys, Hélène1https://orcid.org/0000-0002-2942-4531Abdi, Malika2Mashura, Getrude3Pinarello, Valérie4Chiweshe, Ngoni5Matope, Gift6https://orcid.org/0000-0002-2615-7394Guerrini, Laure7https://orcid.org/0000-0002-4122-8023Pfukenyi, Davies8Cappelle, Julien9https://orcid.org/0000-0001-7668-1971Mwandiringana, Ellen10https://orcid.org/0000-0002-1130-4988Missé, Dorothée11Gori, Elizabeth12Bourgarel, Mathieu13https://orcid.org/0000-0001-9774-7669Liégeois, Florian14https://orcid.org/0000-0003-1048-0661Faculty of Veterinary Science-Biotechnology Center, University of Zimbabwe, P.O. Box MP167 Harare, ZimbabweASTRE, Univ Montpellier, CIRAD, INRAE, Montpellier, France; CIRAD, UMR ASTRE, Montpellier, France; CIRAD, UMR ASTRE, Harare, ZimbabweMIVEGEC, University of Montpellier, IRD, CNRS, 34394 Montpellier, FranceFaculty of Veterinary Science-Biotechnology Center, University of Zimbabwe, P.O. Box MP167 Harare, ZimbabweFaculty of Veterinary Science-Biotechnology Center, University of Zimbabwe, P.O. Box MP167 Harare, Zimbabwe; ASTRE, Univ Montpellier, CIRAD, INRAE, Montpellier, France; CIRAD, UMR ASTRE, Montpellier, France; CIRAD, UMR ASTRE, Harare, ZimbabweCIRAD, UMR ASTRE, Harare, ZimbabweFaculty of Veterinary Science-Biotechnology Center, University of Zimbabwe, P.O. Box MP167 Harare, ZimbabweASTRE, Univ Montpellier, CIRAD, INRAE, Montpellier, France; CIRAD, UMR ASTRE, Montpellier, FranceFaculty of Veterinary Science-Biotechnology Center, University of Zimbabwe, P.O. Box MP167 Harare, Zimbabwe; Faculty of Animal and Veterinary Sciences, Botswana University of Agriculture and Natural Resources, Private Bag 0027, Gaborone, BotswanaASTRE, Univ Montpellier, CIRAD, INRAE, Montpellier, France; CIRAD, UMR ASTRE, Montpellier, FranceFaculty of Veterinary Science-Biotechnology Center, University of Zimbabwe, P.O. Box MP167 Harare, ZimbabweASTRE, Univ Montpellier, CIRAD, INRAE, Montpellier, FranceFaculty of Veterinary Science-Biotechnology Center, University of Zimbabwe, P.O. Box MP167 Harare, Zimbabwe; Department of Biochemistry, Molecular Biology and Genetics, University of Rwanda, RwandaASTRE, Univ Montpellier, CIRAD, INRAE, Montpellier, France; CIRAD, UMR ASTRE, Montpellier, France; CIRAD, UMR ASTRE, Harare, ZimbabweFaculty of Veterinary Science-Biotechnology Center, University of Zimbabwe, P.O. Box MP167 Harare, Zimbabwe; MIVEGEC, University of Montpellier, IRD, CNRS, 34394 Montpellier, FranceAstroviruses (AstVs) have been discovered in over 80 animal species including diverse bat species and avian species. A study on Astrovirus circulation and diversity in different insectivorous and frugivorous chiropteran species roosting in trees, caves and building basements was carried out at 11 different sites across Zimbabwe. Pooled and individual faecal sampling methods were used for this study, with collection dates ranging from June 2016 to July 2021. In two sites, Magweto and Chirundu, sampling was carried out at monthly intervals from August 2020 to July 2021. Astroviruses and bat mitochondrial genes were amplified using pan-AstVs and CytB /12S RNA PCR systems respectively. Phylogenetic analysis of the RdRp Astrovirus sequences revealed a high genetic diversity of astroviruses. All the bat astroviruses tested in this study clustered with the Mamastrovirus genus. Two distinct groups of amplified sequences were identified. One group was comprised of sequences isolated from Hipposideros, Rhinolophus and Eidolon helvum spp. clustered with Human Astrovirus strains: HuAstV types1-6, HuAstV-MLB1-3 and HuAstV-VA-1. The second group comprising the majority of the sequences clustered with different strains of Bat AstVs. Results from the longitudinal study at Magweto and Chirundu showed an overall AstV prevalence of 13.7% and 10.4% respectively. Peaks of AstV infection at Chirundu coincided with the period when juveniles are 4-6 months old. By combining data from our previous work on Coronaviruses, we also anaylzed co-infection of bats with Coronaviruses and Astroviruses at Magweto and Chirundu sites for which the prevalence of co-infection was 2.6% and 3.5% respectively. https://peercommunityjournal.org/articles/10.24072/pcjournal.340/AstrovirusBatsZimbabwePhylogeny |
| spellingShingle | Chidoti, Vimbiso De Nys, Hélène Abdi, Malika Mashura, Getrude Pinarello, Valérie Chiweshe, Ngoni Matope, Gift Guerrini, Laure Pfukenyi, Davies Cappelle, Julien Mwandiringana, Ellen Missé, Dorothée Gori, Elizabeth Bourgarel, Mathieu Liégeois, Florian Longitudinal Survey of Astrovirus infection in different bat species in Zimbabwe: Evidence of high genetic Astrovirus diversity Peer Community Journal Astrovirus Bats Zimbabwe Phylogeny |
| title | Longitudinal Survey of Astrovirus infection in different bat species in Zimbabwe: Evidence of high genetic Astrovirus diversity
|
| title_full | Longitudinal Survey of Astrovirus infection in different bat species in Zimbabwe: Evidence of high genetic Astrovirus diversity
|
| title_fullStr | Longitudinal Survey of Astrovirus infection in different bat species in Zimbabwe: Evidence of high genetic Astrovirus diversity
|
| title_full_unstemmed | Longitudinal Survey of Astrovirus infection in different bat species in Zimbabwe: Evidence of high genetic Astrovirus diversity
|
| title_short | Longitudinal Survey of Astrovirus infection in different bat species in Zimbabwe: Evidence of high genetic Astrovirus diversity
|
| title_sort | longitudinal survey of astrovirus infection in different bat species in zimbabwe evidence of high genetic astrovirus diversity |
| topic | Astrovirus Bats Zimbabwe Phylogeny |
| url | https://peercommunityjournal.org/articles/10.24072/pcjournal.340/ |
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