Reduction of nutrients concentration in culture medium has no effect on bovine embryo production, pregnancy and birth rates
Abstract To improve the quality of in vitro produced (IVP) embryos and reduce pregnancy losses, we proposed to reduce the components of the synthetic oviduct fluid (SOF) medium by 0% (SOF100), 50% (SOF50), and 75% (SOF25). First, embryos produced under these three treatments were evaluated for produ...
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Nature Portfolio
2025-02-01
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| Online Access: | https://doi.org/10.1038/s41598-025-88864-x |
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| author | Ligiane de Oliveira Leme Mauricio Machaim Franco Otávio Augusto de Faria Alexandre Rodrigues Caetano Juliana Gonçalves de Souza Luciano de Rezende Carvalheira Emivaldo de Siqueira Filho Margot Alves Nunes Dode |
| author_facet | Ligiane de Oliveira Leme Mauricio Machaim Franco Otávio Augusto de Faria Alexandre Rodrigues Caetano Juliana Gonçalves de Souza Luciano de Rezende Carvalheira Emivaldo de Siqueira Filho Margot Alves Nunes Dode |
| author_sort | Ligiane de Oliveira Leme |
| collection | DOAJ |
| description | Abstract To improve the quality of in vitro produced (IVP) embryos and reduce pregnancy losses, we proposed to reduce the components of the synthetic oviduct fluid (SOF) medium by 0% (SOF100), 50% (SOF50), and 75% (SOF25). First, embryos produced under these three treatments were evaluated for production, quality, lipid content, gene expression, and methylation patterns. The results indicated that all parameters analyzed were similar across all treatments (P > 0.05), suggesting that reducing media components does not affect embryo development and quality. Subsequently, we selected SOF25 for comparison with SOF100 in a commercial laboratory setting, evaluating embryo production, response to cryopreservation, gestation rate, and offspring birth. The data demonstrated that a 75% reduction in SOF medium components did not affect embryo development, quality, pregnancy rate, embryonic losses between 30 and 60 days, or birth rate (P > 0.05). To our knowledge, this is the first report on the pregnancy and birth rates of bovine blastocysts produced in media with nutrient concentrations as low as 25%. These results introduce novel cultural conditions that can be immediately incorporated into the IVF routine. |
| format | Article |
| id | doaj-art-a5e65edc89c34d6d8c01e96a50d5a23b |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-a5e65edc89c34d6d8c01e96a50d5a23b2025-02-09T12:31:15ZengNature PortfolioScientific Reports2045-23222025-02-0115111110.1038/s41598-025-88864-xReduction of nutrients concentration in culture medium has no effect on bovine embryo production, pregnancy and birth ratesLigiane de Oliveira Leme0Mauricio Machaim Franco1Otávio Augusto de Faria2Alexandre Rodrigues Caetano3Juliana Gonçalves de Souza4Luciano de Rezende Carvalheira5Emivaldo de Siqueira Filho6Margot Alves Nunes Dode7Fundação de Amparo à Pesquisa do Distrito Federal – FAPDFEmbrapa Recursos Genéticos e BiotecnologiaUniversidade de BrasíliaEmbrapa Recursos Genéticos e BiotecnologiaUniversidade Federal de GoiásEmbriotecEmbriotecEmbrapa Recursos Genéticos e BiotecnologiaAbstract To improve the quality of in vitro produced (IVP) embryos and reduce pregnancy losses, we proposed to reduce the components of the synthetic oviduct fluid (SOF) medium by 0% (SOF100), 50% (SOF50), and 75% (SOF25). First, embryos produced under these three treatments were evaluated for production, quality, lipid content, gene expression, and methylation patterns. The results indicated that all parameters analyzed were similar across all treatments (P > 0.05), suggesting that reducing media components does not affect embryo development and quality. Subsequently, we selected SOF25 for comparison with SOF100 in a commercial laboratory setting, evaluating embryo production, response to cryopreservation, gestation rate, and offspring birth. The data demonstrated that a 75% reduction in SOF medium components did not affect embryo development, quality, pregnancy rate, embryonic losses between 30 and 60 days, or birth rate (P > 0.05). To our knowledge, this is the first report on the pregnancy and birth rates of bovine blastocysts produced in media with nutrient concentrations as low as 25%. These results introduce novel cultural conditions that can be immediately incorporated into the IVF routine.https://doi.org/10.1038/s41598-025-88864-xCulture mediaBovine pregnancy rateBovine birth rateMedium nutrients reduction |
| spellingShingle | Ligiane de Oliveira Leme Mauricio Machaim Franco Otávio Augusto de Faria Alexandre Rodrigues Caetano Juliana Gonçalves de Souza Luciano de Rezende Carvalheira Emivaldo de Siqueira Filho Margot Alves Nunes Dode Reduction of nutrients concentration in culture medium has no effect on bovine embryo production, pregnancy and birth rates Scientific Reports Culture media Bovine pregnancy rate Bovine birth rate Medium nutrients reduction |
| title | Reduction of nutrients concentration in culture medium has no effect on bovine embryo production, pregnancy and birth rates |
| title_full | Reduction of nutrients concentration in culture medium has no effect on bovine embryo production, pregnancy and birth rates |
| title_fullStr | Reduction of nutrients concentration in culture medium has no effect on bovine embryo production, pregnancy and birth rates |
| title_full_unstemmed | Reduction of nutrients concentration in culture medium has no effect on bovine embryo production, pregnancy and birth rates |
| title_short | Reduction of nutrients concentration in culture medium has no effect on bovine embryo production, pregnancy and birth rates |
| title_sort | reduction of nutrients concentration in culture medium has no effect on bovine embryo production pregnancy and birth rates |
| topic | Culture media Bovine pregnancy rate Bovine birth rate Medium nutrients reduction |
| url | https://doi.org/10.1038/s41598-025-88864-x |
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