Evidence of vancomycin-resistant Staphylococcus aureus, multidrug-resistant S. aureus, and Enterococcus faecium-causing mastitis in Thailand and Cambodia
Background and Aim: Bovine mastitis, an inflammatory condition of the mammary gland, is a critical economic issue in the dairy industry. Antimicrobial resistance (AMR) to mastitis-causing pathogens poses a significant threat to dairy operations in Thailand and Cambodia. This study investigates the A...
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Veterinary World
2025-01-01
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| Series: | Veterinary World |
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| Online Access: | https://www.veterinaryworld.org/Vol.18/January-2025/22.pdf |
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| author | Sambo Na Montira Intanon Anyaphat Srithanasuwan Wasana Chaisri Witaya Suriyasathaporn |
| author_facet | Sambo Na Montira Intanon Anyaphat Srithanasuwan Wasana Chaisri Witaya Suriyasathaporn |
| author_sort | Sambo Na |
| collection | DOAJ |
| description | Background and Aim: Bovine mastitis, an inflammatory condition of the mammary gland, is a critical economic issue in the dairy industry. Antimicrobial resistance (AMR) to mastitis-causing pathogens poses a significant threat to dairy operations in Thailand and Cambodia. This study investigates the AMR of mastitis pathogens in Thailand and Cambodia. It focuses on detecting methicillin-resistant Staphylococcus aureus, vancomycin-resistant S. aureus (VRSA), and vancomycin-resistant enterococci by identifying the presence of mecA, vanA, and vanB genes in bacterial isolates.
Materials and Methods: A total of 65 bacterial isolates (55 S. aureus from Thailand and 10 Enterococcus faecium from Thailand and Cambodia) were analyzed. Disk diffusion tests were conducted for antibiotic susceptibility, and polymerase chain reaction was employed to detect AMR genes.
Results: S. aureus isolates showed resistance to penicillin (21.8%), tetracycline (9.1%), and gentamycin (7.3%). Three isolates were identified as multidrug-resistant (MDR), resistant to tetracycline, gentamycin, and penicillin. E. faecium isolates exhibited high resistance to tetracycline (100%) and penicillin (90%), with 60% classified as MDR. Phenotypic analysis identified VRSA in 11% of S. aureus isolates. However, mecA, vanA, and vanB genes were not detected in any isolate.
Conclusion: Mastitis pathogens in this study pose significant AMR challenges, especially with MDR S. aureus and E. faecium, and phenotypically VRSA without the vanA gene. The findings highlight the need for judicious antibiotic use in dairy farms and further studies with broader sampling. |
| format | Article |
| id | doaj-art-a8424f1f75f84381a8a779b011410bc5 |
| institution | Kabale University |
| issn | 0972-8988 2231-0916 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj-art-a8424f1f75f84381a8a779b011410bc52025-02-07T12:56:03ZengVeterinary WorldVeterinary World0972-89882231-09162025-01-0118120220910.14202/vetworld.2025.202-209Evidence of vancomycin-resistant Staphylococcus aureus, multidrug-resistant S. aureus, and Enterococcus faecium-causing mastitis in Thailand and CambodiaSambo Na0https://orcid.org/0009-0003-2763-8004Montira Intanon1https://orcid.org/0000-0001-7258-6897Anyaphat Srithanasuwan2https://orcid.org/0000-0003-2837-7187Wasana Chaisri3https://orcid.org/0000-0002-7648-2662Witaya Suriyasathaporn4https://orcid.org/0000-0002-2327-195XMaster’s Degree Program in Veterinary Science, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.School of Veterinary Medicine, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand; Research Center of Producing and Development of Products and Innovations for Animal Health, Chiang Mai University, Chiang Mai, 50100, Thailand.School of Veterinary Medicine, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand; Department of Animal Sciences, Wageningen University, 6708, PB Wageningen, The Netherlands.School of Veterinary Medicine, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand; Research Center of Producing and Development of Products and Innovations for Animal Health, Chiang Mai University, Chiang Mai, 50100, Thailand.School of Veterinary Medicine, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand; Research Center of Producing and Development of Products and Innovations for Animal Health, Chiang Mai University, Chiang Mai, 50100, Thailand; Cambodia-Campuses, Asian Satellite Campuses Institute, Nagoya University, Japan.Background and Aim: Bovine mastitis, an inflammatory condition of the mammary gland, is a critical economic issue in the dairy industry. Antimicrobial resistance (AMR) to mastitis-causing pathogens poses a significant threat to dairy operations in Thailand and Cambodia. This study investigates the AMR of mastitis pathogens in Thailand and Cambodia. It focuses on detecting methicillin-resistant Staphylococcus aureus, vancomycin-resistant S. aureus (VRSA), and vancomycin-resistant enterococci by identifying the presence of mecA, vanA, and vanB genes in bacterial isolates. Materials and Methods: A total of 65 bacterial isolates (55 S. aureus from Thailand and 10 Enterococcus faecium from Thailand and Cambodia) were analyzed. Disk diffusion tests were conducted for antibiotic susceptibility, and polymerase chain reaction was employed to detect AMR genes. Results: S. aureus isolates showed resistance to penicillin (21.8%), tetracycline (9.1%), and gentamycin (7.3%). Three isolates were identified as multidrug-resistant (MDR), resistant to tetracycline, gentamycin, and penicillin. E. faecium isolates exhibited high resistance to tetracycline (100%) and penicillin (90%), with 60% classified as MDR. Phenotypic analysis identified VRSA in 11% of S. aureus isolates. However, mecA, vanA, and vanB genes were not detected in any isolate. Conclusion: Mastitis pathogens in this study pose significant AMR challenges, especially with MDR S. aureus and E. faecium, and phenotypically VRSA without the vanA gene. The findings highlight the need for judicious antibiotic use in dairy farms and further studies with broader sampling.https://www.veterinaryworld.org/Vol.18/January-2025/22.pdfantimicrobial resistancedairy farmsmastitismethicillin-resistant staphylococcus aureusmultidrug resistancevancomycin-resistant enterococcivancomycin-resistant staphylococcus aureus |
| spellingShingle | Sambo Na Montira Intanon Anyaphat Srithanasuwan Wasana Chaisri Witaya Suriyasathaporn Evidence of vancomycin-resistant Staphylococcus aureus, multidrug-resistant S. aureus, and Enterococcus faecium-causing mastitis in Thailand and Cambodia Veterinary World antimicrobial resistance dairy farms mastitis methicillin-resistant staphylococcus aureus multidrug resistance vancomycin-resistant enterococci vancomycin-resistant staphylococcus aureus |
| title | Evidence of vancomycin-resistant Staphylococcus aureus, multidrug-resistant S. aureus, and Enterococcus faecium-causing mastitis in Thailand and Cambodia |
| title_full | Evidence of vancomycin-resistant Staphylococcus aureus, multidrug-resistant S. aureus, and Enterococcus faecium-causing mastitis in Thailand and Cambodia |
| title_fullStr | Evidence of vancomycin-resistant Staphylococcus aureus, multidrug-resistant S. aureus, and Enterococcus faecium-causing mastitis in Thailand and Cambodia |
| title_full_unstemmed | Evidence of vancomycin-resistant Staphylococcus aureus, multidrug-resistant S. aureus, and Enterococcus faecium-causing mastitis in Thailand and Cambodia |
| title_short | Evidence of vancomycin-resistant Staphylococcus aureus, multidrug-resistant S. aureus, and Enterococcus faecium-causing mastitis in Thailand and Cambodia |
| title_sort | evidence of vancomycin resistant staphylococcus aureus multidrug resistant s aureus and enterococcus faecium causing mastitis in thailand and cambodia |
| topic | antimicrobial resistance dairy farms mastitis methicillin-resistant staphylococcus aureus multidrug resistance vancomycin-resistant enterococci vancomycin-resistant staphylococcus aureus |
| url | https://www.veterinaryworld.org/Vol.18/January-2025/22.pdf |
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