Oxidative alterations in exfoliated oral mucosa cells of patients with major depressive disorder
Objectives: This study aimed to investigate oxidative stress markers in the oral mucosal cells of individuals diagnosed with major depressive disorder (MDD). Methods: A case-control design was used, including twenty patients diagnosed with MDD, based on the Diagnostic and Statistical Manual of Menta...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426825000284 |
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| author | Lukas Mendes de Abreu Cintia Rodrigues da Silva Ana Laura Ferreira Bortoleto Giovana Barros Nunes Matheus Martins Gracia Rafael Akira Tzanno Murayama Daniel Galera Bernabé Gisele Zoccal Mingoti |
| author_facet | Lukas Mendes de Abreu Cintia Rodrigues da Silva Ana Laura Ferreira Bortoleto Giovana Barros Nunes Matheus Martins Gracia Rafael Akira Tzanno Murayama Daniel Galera Bernabé Gisele Zoccal Mingoti |
| author_sort | Lukas Mendes de Abreu |
| collection | DOAJ |
| description | Objectives: This study aimed to investigate oxidative stress markers in the oral mucosal cells of individuals diagnosed with major depressive disorder (MDD). Methods: A case-control design was used, including twenty patients diagnosed with MDD, based on the Diagnostic and Statistical Manual of Mental Disorders (DSM-5) criteria, and twenty healthy controls. Oral exfoliated cells were collected from all participants. Intracellular levels of reactive oxygen species (ROS), mitochondrial membrane potential (ΔΨm), caspase-3 and -7 activity, and reduced glutathione (GSH) were measured in Arbitrary Fluorescence Units (AFU). Results: The MDD group demonstrated significantly elevated intracellular ROS levels (p = 0.0012) and caspase-3 and -7 activity (p = 0.0171) in comparison to the control group. Additionally, a decrease in ΔΨm expression was observed in the oral cells of MDD patients (p = 0.0265), whereas GSH expression levels did not differ significantly between the two groups (p = 0.8908). Conclusions: The findings indicate heightened oxidative stress in the oral exfoliated cells of individuals with MDD. This study supports the potential use of oral cells as a non-invasive biomarker source for assessing oxidative stress in depressive disorders. |
| format | Article |
| id | doaj-art-a8682c398a8247c09421b48d41a74638 |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-a8682c398a8247c09421b48d41a746382025-02-12T05:31:02ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682025-03-01152256261Oxidative alterations in exfoliated oral mucosa cells of patients with major depressive disorderLukas Mendes de Abreu0Cintia Rodrigues da Silva1Ana Laura Ferreira Bortoleto2Giovana Barros Nunes3Matheus Martins Gracia4Rafael Akira Tzanno Murayama5Daniel Galera Bernabé6Gisele Zoccal Mingoti7Oral Oncology Center, São Paulo State University (Unesp), School of Dentistry, Campus Araçatuba, São Paulo, Brazil; Corresponding author.São Paulo State University (UNESP), Graduate Program in Veterinary Medicine, School of Agrarian and Veterinary Sciences, Campus Jaboticabal, São Paulo, BrazilOral Oncology Center, São Paulo State University (Unesp), School of Dentistry, Campus Araçatuba, São Paulo, BrazilSão Paulo State University (UNESP), Graduate Program in Veterinary Medicine, School of Agrarian and Veterinary Sciences, Campus Jaboticabal, São Paulo, BrazilSpecialized Psychosocial Care Center (CEAPS), Araçatuba, São Paulo, BrazilOral Oncology Center, São Paulo State University (Unesp), School of Dentistry, Campus Araçatuba, São Paulo, BrazilOral Oncology Center, São Paulo State University (Unesp), School of Dentistry, Campus Araçatuba, São Paulo, Brazil; Department of Diagnosis and Surgery, São Paulo State University (Unesp), School of Dentistry, 1193 José Bonifácio St Araçatuba, SP 16050-015, São Paulo, BrazilOral Oncology Center, São Paulo State University (Unesp), School of Dentistry, Campus Araçatuba, São Paulo, Brazil; São Paulo State University (UNESP), Graduate Program in Veterinary Medicine, School of Agrarian and Veterinary Sciences, Campus Jaboticabal, São Paulo, Brazil; São Paulo State University (UNESP), School of Veterinary Medicine, Laboratory of Reproductive Physiology, Campus Araçatuba, São Paulo, Brazil; Corresponding author. Department of Animal Production and Health, School of Veterinary Medicine, Laboratory of Reproductive Physiology, São Paulo State University (UNESP), Clóvis Pestana Street, 793 Araçatuba, 16050-680, São Paulo, Brazil.Objectives: This study aimed to investigate oxidative stress markers in the oral mucosal cells of individuals diagnosed with major depressive disorder (MDD). Methods: A case-control design was used, including twenty patients diagnosed with MDD, based on the Diagnostic and Statistical Manual of Mental Disorders (DSM-5) criteria, and twenty healthy controls. Oral exfoliated cells were collected from all participants. Intracellular levels of reactive oxygen species (ROS), mitochondrial membrane potential (ΔΨm), caspase-3 and -7 activity, and reduced glutathione (GSH) were measured in Arbitrary Fluorescence Units (AFU). Results: The MDD group demonstrated significantly elevated intracellular ROS levels (p = 0.0012) and caspase-3 and -7 activity (p = 0.0171) in comparison to the control group. Additionally, a decrease in ΔΨm expression was observed in the oral cells of MDD patients (p = 0.0265), whereas GSH expression levels did not differ significantly between the two groups (p = 0.8908). Conclusions: The findings indicate heightened oxidative stress in the oral exfoliated cells of individuals with MDD. This study supports the potential use of oral cells as a non-invasive biomarker source for assessing oxidative stress in depressive disorders.http://www.sciencedirect.com/science/article/pii/S2212426825000284Major depressive disorderDepressionOxidative stressOral mucosaOral disease |
| spellingShingle | Lukas Mendes de Abreu Cintia Rodrigues da Silva Ana Laura Ferreira Bortoleto Giovana Barros Nunes Matheus Martins Gracia Rafael Akira Tzanno Murayama Daniel Galera Bernabé Gisele Zoccal Mingoti Oxidative alterations in exfoliated oral mucosa cells of patients with major depressive disorder Journal of Oral Biology and Craniofacial Research Major depressive disorder Depression Oxidative stress Oral mucosa Oral disease |
| title | Oxidative alterations in exfoliated oral mucosa cells of patients with major depressive disorder |
| title_full | Oxidative alterations in exfoliated oral mucosa cells of patients with major depressive disorder |
| title_fullStr | Oxidative alterations in exfoliated oral mucosa cells of patients with major depressive disorder |
| title_full_unstemmed | Oxidative alterations in exfoliated oral mucosa cells of patients with major depressive disorder |
| title_short | Oxidative alterations in exfoliated oral mucosa cells of patients with major depressive disorder |
| title_sort | oxidative alterations in exfoliated oral mucosa cells of patients with major depressive disorder |
| topic | Major depressive disorder Depression Oxidative stress Oral mucosa Oral disease |
| url | http://www.sciencedirect.com/science/article/pii/S2212426825000284 |
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