Antioxidant activity of ecoenzyme derived from Siamese orange peel (Citrus nobilis) at different fermentation duration
Background: Ecoenzyme is an organic fermentation product derived from fruit peel waste, offering zero-waste system benefits along with antibacterial, insecticidal, and cleaning properties. Kintamani, a major producer of Siamese oranges (Citrus nobilis) in Bali, generates significant orange peel was...
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| Format: | Article |
| Language: | English |
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Indonesian Society for Biochemistry and Molecular Biology
2024-12-01
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| Series: | Acta Biochimica Indonesiana |
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| Online Access: | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/173 |
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| _version_ | 1825199595432443904 |
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| author | Anak Agung Ayu Radismarani Putri Pande Ayu Naya Kasih Permatananda I Gde Suranaya Pandit Asri Lestarini |
| author_facet | Anak Agung Ayu Radismarani Putri Pande Ayu Naya Kasih Permatananda I Gde Suranaya Pandit Asri Lestarini |
| author_sort | Anak Agung Ayu Radismarani Putri |
| collection | DOAJ |
| description |
Background: Ecoenzyme is an organic fermentation product derived from fruit peel waste, offering zero-waste system benefits along with antibacterial, insecticidal, and cleaning properties. Kintamani, a major producer of Siamese oranges (Citrus nobilis) in Bali, generates significant orange peel waste suitable for ecoenzyme production. However, the antioxidant potential of ecoenzymes remains poorly studied.
Objective: This study aimed to evaluate the antioxidant activity of ecoenzymes produced from Kintamani Siamese orange peel waste, enhancing their beneficial value.
Methods: This experimental study assessed and compared the antioxidant activity of ecoenzymes fermented for 10 days, 1 month, and 3 months. Antioxidant activity was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, with results expressed as IC50 values.
Results: Antioxidant activity, classified according to Blois criteria, showed the highest activity after three months of fermentation. The mean IC50 values were 53,612 ppm for 10-day fermentation; 18,753 ppm for 1-month fermentation; and 6,727 ppm for 3-month fermentation.
Conclusion: Ecoenzymes derived from Kintamani Siamese orange peel waste exhibited very weak antioxidant activity across all fermentation durations. A 3-month fermentation period is recommended to achieve the highest antioxidant activity compared to shorter durations.
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| format | Article |
| id | doaj-art-ac39cf0796f146f3852774d90b50238a |
| institution | Kabale University |
| issn | 2654-6108 2654-3222 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Indonesian Society for Biochemistry and Molecular Biology |
| record_format | Article |
| series | Acta Biochimica Indonesiana |
| spelling | doaj-art-ac39cf0796f146f3852774d90b50238a2025-02-08T03:04:42ZengIndonesian Society for Biochemistry and Molecular BiologyActa Biochimica Indonesiana2654-61082654-32222024-12-017210.32889/actabioina.173Antioxidant activity of ecoenzyme derived from Siamese orange peel (Citrus nobilis) at different fermentation durationAnak Agung Ayu Radismarani Putri0Pande Ayu Naya Kasih Permatananda1I Gde Suranaya Pandit2Asri Lestarini3Faculty of Medicine and Health Science Universitas Warmadewa, 80239, IndonesiaDepartment of Pharmacology, Faculty of Medicine and Health Science, Universitas Warmadewa, 80239, IndonesiaDepartment of Food Technology, Faculty of Agriculture, Universitas Warmadewa, 80239, IndonesiaDepartment of Biochemistry, Faculty of Medicine and Health Science, Universitas Warmadewa, 80239, Indonesia Background: Ecoenzyme is an organic fermentation product derived from fruit peel waste, offering zero-waste system benefits along with antibacterial, insecticidal, and cleaning properties. Kintamani, a major producer of Siamese oranges (Citrus nobilis) in Bali, generates significant orange peel waste suitable for ecoenzyme production. However, the antioxidant potential of ecoenzymes remains poorly studied. Objective: This study aimed to evaluate the antioxidant activity of ecoenzymes produced from Kintamani Siamese orange peel waste, enhancing their beneficial value. Methods: This experimental study assessed and compared the antioxidant activity of ecoenzymes fermented for 10 days, 1 month, and 3 months. Antioxidant activity was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, with results expressed as IC50 values. Results: Antioxidant activity, classified according to Blois criteria, showed the highest activity after three months of fermentation. The mean IC50 values were 53,612 ppm for 10-day fermentation; 18,753 ppm for 1-month fermentation; and 6,727 ppm for 3-month fermentation. Conclusion: Ecoenzymes derived from Kintamani Siamese orange peel waste exhibited very weak antioxidant activity across all fermentation durations. A 3-month fermentation period is recommended to achieve the highest antioxidant activity compared to shorter durations. https://pbbmi.org/newjurnal/index.php/actabioina/article/view/173antioxidant activitySiamese OrangeOrange Peel WasteEcoenzyme |
| spellingShingle | Anak Agung Ayu Radismarani Putri Pande Ayu Naya Kasih Permatananda I Gde Suranaya Pandit Asri Lestarini Antioxidant activity of ecoenzyme derived from Siamese orange peel (Citrus nobilis) at different fermentation duration Acta Biochimica Indonesiana antioxidant activity Siamese Orange Orange Peel Waste Ecoenzyme |
| title | Antioxidant activity of ecoenzyme derived from Siamese orange peel (Citrus nobilis) at different fermentation duration |
| title_full | Antioxidant activity of ecoenzyme derived from Siamese orange peel (Citrus nobilis) at different fermentation duration |
| title_fullStr | Antioxidant activity of ecoenzyme derived from Siamese orange peel (Citrus nobilis) at different fermentation duration |
| title_full_unstemmed | Antioxidant activity of ecoenzyme derived from Siamese orange peel (Citrus nobilis) at different fermentation duration |
| title_short | Antioxidant activity of ecoenzyme derived from Siamese orange peel (Citrus nobilis) at different fermentation duration |
| title_sort | antioxidant activity of ecoenzyme derived from siamese orange peel citrus nobilis at different fermentation duration |
| topic | antioxidant activity Siamese Orange Orange Peel Waste Ecoenzyme |
| url | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/173 |
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