Biofilmed-PGPR: Next-Generation Bioinoculant for Plant Growth Promotion in Rice under Changing Climate
The exopolysaccharide matrix of diazotrophic cyanobacteria was used to integrate phosphorus (P) and potassium (K) solubilizing bacteria, enhancing the survival of plant growth-promoting rhizobacteria, and ultimately the survival of bacteria in the rhizosphere for better plant growth. A new biofilm-b...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Rice Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1672630824000933 |
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| author | Jeberlin Prabina Bright Hemant S. Maheshwari Sugitha Thangappan Kahkashan Perveen Najat A. Bukhari Debasis Mitra Riyaz Sayyed Andrea Mastinu |
| author_facet | Jeberlin Prabina Bright Hemant S. Maheshwari Sugitha Thangappan Kahkashan Perveen Najat A. Bukhari Debasis Mitra Riyaz Sayyed Andrea Mastinu |
| author_sort | Jeberlin Prabina Bright |
| collection | DOAJ |
| description | The exopolysaccharide matrix of diazotrophic cyanobacteria was used to integrate phosphorus (P) and potassium (K) solubilizing bacteria, enhancing the survival of plant growth-promoting rhizobacteria, and ultimately the survival of bacteria in the rhizosphere for better plant growth. A new biofilm-based formulation comprising the diazotrophic cyanobacteria Anabaena AMP2, P-solubilizing Bacillus megaterium var. phosphaticum PB1, and K-solubilizing Rhizobium pusense KRBKKM1 was tested for efficacy in rice. The growth medium with half-strength BG-11 medium supplemented with 3% glucose showed best for biofilm formation under in vitro conditions. Analysis of the methanolic extract of the cyanobacterial- bacterial biofilm (CBB) showed the activity of antioxidants, such as 2-methoxy phenol and pentadecane, which are proven to improve plant-microbe interactions and plant growth, respectively. Treatment of rice seeds with CBB extract at 100 mL/kg or 200 mL/kg showed significant enhancement in germination rate and seedling length. Therefore, a pot culture experiment with the CBB formulations was carried out, and different growth and yield parameters were recorded. Principal component analysis showed that plant growth, yield, soil dehydrogenase activity, and soil chlorophyll content were positively correlated with rice plants amended with vermiculite-based CBB at 2 kg/hm2 followed by a spray with aqueous CBB formulation at 5 mL/L at 15 and 30 d after rice transplanting grown with a 25% reduced level of nitrogen/phosphorus/potassium chemical fertilizers than the recommended dose. Further, Pearson correlation analysis showed that yield was positively correlated with soil dehydrogenase (r = 0.92**) and soil chlorophyll content (r = 0.96**). We concluded that CBB could be used as a novel biofilm-based bio-inoculant to increase rice productivity and crop fitness as a component in integrated nutrient management and sustainable organic farming strategies with reduced chemical fertilizers. |
| format | Article |
| id | doaj-art-ac8584da6a684383b766f4d49deed50e |
| institution | Kabale University |
| issn | 1672-6308 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Rice Science |
| spelling | doaj-art-ac8584da6a684383b766f4d49deed50e2025-02-12T05:30:49ZengElsevierRice Science1672-63082025-01-0132194106Biofilmed-PGPR: Next-Generation Bioinoculant for Plant Growth Promotion in Rice under Changing ClimateJeberlin Prabina Bright0Hemant S. Maheshwari1Sugitha Thangappan2Kahkashan Perveen3Najat A. Bukhari4Debasis Mitra5Riyaz Sayyed6Andrea Mastinu7Department of Soil Science and Agricultural Chemistry, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Killikulam 628252, India; Corresponding author.Indian Council of Agricultural Research (ICAR)-Indian Institute of Soybean Research, Indore 452001, IndiaKarunya Institute of Technology and Sciences, School of Agricultural Sciences, Karunya Nagar, Coimbatore 641114, IndiaDepartment of Botany & Microbiology, College of Science, King Saud University, Riyadh-11495, Saudi ArabiaDepartment of Botany & Microbiology, College of Science, King Saud University, Riyadh-11495, Saudi ArabiaDepartment of Microbiology, Graphic Era (Deemed to be University), Clement Town, Dehradun 248002, IndiaDepartment of Biological Sciences and Chemistry, College of Arts and Science, University of Nizwa, Nizwa 616, Sultanate of OmanDepartment of Molecular and Translational Medicine, Division of Pharmacology, University of Brescia, Brescia 25123, Italy; Corresponding author.The exopolysaccharide matrix of diazotrophic cyanobacteria was used to integrate phosphorus (P) and potassium (K) solubilizing bacteria, enhancing the survival of plant growth-promoting rhizobacteria, and ultimately the survival of bacteria in the rhizosphere for better plant growth. A new biofilm-based formulation comprising the diazotrophic cyanobacteria Anabaena AMP2, P-solubilizing Bacillus megaterium var. phosphaticum PB1, and K-solubilizing Rhizobium pusense KRBKKM1 was tested for efficacy in rice. The growth medium with half-strength BG-11 medium supplemented with 3% glucose showed best for biofilm formation under in vitro conditions. Analysis of the methanolic extract of the cyanobacterial- bacterial biofilm (CBB) showed the activity of antioxidants, such as 2-methoxy phenol and pentadecane, which are proven to improve plant-microbe interactions and plant growth, respectively. Treatment of rice seeds with CBB extract at 100 mL/kg or 200 mL/kg showed significant enhancement in germination rate and seedling length. Therefore, a pot culture experiment with the CBB formulations was carried out, and different growth and yield parameters were recorded. Principal component analysis showed that plant growth, yield, soil dehydrogenase activity, and soil chlorophyll content were positively correlated with rice plants amended with vermiculite-based CBB at 2 kg/hm2 followed by a spray with aqueous CBB formulation at 5 mL/L at 15 and 30 d after rice transplanting grown with a 25% reduced level of nitrogen/phosphorus/potassium chemical fertilizers than the recommended dose. Further, Pearson correlation analysis showed that yield was positively correlated with soil dehydrogenase (r = 0.92**) and soil chlorophyll content (r = 0.96**). We concluded that CBB could be used as a novel biofilm-based bio-inoculant to increase rice productivity and crop fitness as a component in integrated nutrient management and sustainable organic farming strategies with reduced chemical fertilizers.http://www.sciencedirect.com/science/article/pii/S1672630824000933cyanobacterial bacterial biofilm formulationricevolatile compoundplant growth promotionreduced fertilizer level |
| spellingShingle | Jeberlin Prabina Bright Hemant S. Maheshwari Sugitha Thangappan Kahkashan Perveen Najat A. Bukhari Debasis Mitra Riyaz Sayyed Andrea Mastinu Biofilmed-PGPR: Next-Generation Bioinoculant for Plant Growth Promotion in Rice under Changing Climate Rice Science cyanobacterial bacterial biofilm formulation rice volatile compound plant growth promotion reduced fertilizer level |
| title | Biofilmed-PGPR: Next-Generation Bioinoculant for Plant Growth Promotion in Rice under Changing Climate |
| title_full | Biofilmed-PGPR: Next-Generation Bioinoculant for Plant Growth Promotion in Rice under Changing Climate |
| title_fullStr | Biofilmed-PGPR: Next-Generation Bioinoculant for Plant Growth Promotion in Rice under Changing Climate |
| title_full_unstemmed | Biofilmed-PGPR: Next-Generation Bioinoculant for Plant Growth Promotion in Rice under Changing Climate |
| title_short | Biofilmed-PGPR: Next-Generation Bioinoculant for Plant Growth Promotion in Rice under Changing Climate |
| title_sort | biofilmed pgpr next generation bioinoculant for plant growth promotion in rice under changing climate |
| topic | cyanobacterial bacterial biofilm formulation rice volatile compound plant growth promotion reduced fertilizer level |
| url | http://www.sciencedirect.com/science/article/pii/S1672630824000933 |
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