Comprehensive analysis of differential mRNA and circRNA profiles in primary and metastatic pancreatic neuroendocrine tumors
The diagnosis of primary pancreatic neuroendocrine tumors (pNETs) presents significant challenges, and metastatic pancreatic neuroendocrine tumors are associated with high mortality. Understanding the characteristics of these tumors, particularly the key molecules involved in metastasis, is essentia...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580825000226 |
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| author | Gang Li Jing Zhang Bentuo Zhang Dan Wang Zequn Wang Yan Pan Lijie Ma |
| author_facet | Gang Li Jing Zhang Bentuo Zhang Dan Wang Zequn Wang Yan Pan Lijie Ma |
| author_sort | Gang Li |
| collection | DOAJ |
| description | The diagnosis of primary pancreatic neuroendocrine tumors (pNETs) presents significant challenges, and metastatic pancreatic neuroendocrine tumors are associated with high mortality. Understanding the characteristics of these tumors, particularly the key molecules involved in metastasis, is essential. To address this, we utilized mRNA expression data from human pNET and metastatic pancreatic tumor tissues available in the GEO database and integrated this data with bioinformatics analyses. And then we collected clinical primary tumor and liver metastasis samples from patients with pNETs, we conducted a comprehensive analysis of circular RNAs (circRNAs) to identify key circRNAs associated with the onset and metastasis of pNETs. We found that in pNET development and metastasis, 11 genes and 14 circRNAs were notably upregulated, while 25 genes and 35 circRNAs were significantly downregulated, compared to nearby non-cancerous tissue. Our analysis of differentially expressed RNA and circRNA genes revealed that tumor cell adhesion and integrin activation, regulated by genes like PIEZO1, IFT74, SKAP1, GPX1, F7, VTN, and OMG, are strongly linked to pNET metastasis. We found that SKAP1 levels are positively associated with tumor progression in pNET patients. Overall, our research indicates that the SKAP1-mediated pathway is crucial in pNET development and metastasis. |
| format | Article |
| id | doaj-art-ad85fc5b1507482d8cb86a5b76db5ff8 |
| institution | Kabale University |
| issn | 2405-5808 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj-art-ad85fc5b1507482d8cb86a5b76db5ff82025-02-09T05:00:36ZengElsevierBiochemistry and Biophysics Reports2405-58082025-03-0141101935Comprehensive analysis of differential mRNA and circRNA profiles in primary and metastatic pancreatic neuroendocrine tumorsGang Li0Jing Zhang1Bentuo Zhang2Dan Wang3Zequn Wang4Yan Pan5Lijie Ma6Department of General Surgery, Peking University Third Hospital, Beijing, 100191, ChinaInner Mongolia Key Laboratory of Molecular Biology, Inner Mongolia Medical University, Hohhot, 010059, ChinaDepartment of Pharmacology, School of Basic Medical Sciences, Health Science Center, Peking University, Beijing, 100191, ChinaDepartment of Pharmacology, School of Basic Medical Sciences, Health Science Center, Peking University, Beijing, 100191, ChinaDepartment of Pharmacology, School of Basic Medical Sciences, Health Science Center, Peking University, Beijing, 100191, ChinaDepartment of Pharmacology, School of Basic Medical Sciences, Health Science Center, Peking University, Beijing, 100191, China; Corresponding author.Inner Mongolia Key Laboratory of Molecular Biology, Inner Mongolia Medical University, Hohhot, 010059, China; Corresponding author.The diagnosis of primary pancreatic neuroendocrine tumors (pNETs) presents significant challenges, and metastatic pancreatic neuroendocrine tumors are associated with high mortality. Understanding the characteristics of these tumors, particularly the key molecules involved in metastasis, is essential. To address this, we utilized mRNA expression data from human pNET and metastatic pancreatic tumor tissues available in the GEO database and integrated this data with bioinformatics analyses. And then we collected clinical primary tumor and liver metastasis samples from patients with pNETs, we conducted a comprehensive analysis of circular RNAs (circRNAs) to identify key circRNAs associated with the onset and metastasis of pNETs. We found that in pNET development and metastasis, 11 genes and 14 circRNAs were notably upregulated, while 25 genes and 35 circRNAs were significantly downregulated, compared to nearby non-cancerous tissue. Our analysis of differentially expressed RNA and circRNA genes revealed that tumor cell adhesion and integrin activation, regulated by genes like PIEZO1, IFT74, SKAP1, GPX1, F7, VTN, and OMG, are strongly linked to pNET metastasis. We found that SKAP1 levels are positively associated with tumor progression in pNET patients. Overall, our research indicates that the SKAP1-mediated pathway is crucial in pNET development and metastasis.http://www.sciencedirect.com/science/article/pii/S2405580825000226Primary pancreatic neuroendocrine tumorLive metastasisRNA sequencingCircRNA microarraysTumor adhesion |
| spellingShingle | Gang Li Jing Zhang Bentuo Zhang Dan Wang Zequn Wang Yan Pan Lijie Ma Comprehensive analysis of differential mRNA and circRNA profiles in primary and metastatic pancreatic neuroendocrine tumors Biochemistry and Biophysics Reports Primary pancreatic neuroendocrine tumor Live metastasis RNA sequencing CircRNA microarrays Tumor adhesion |
| title | Comprehensive analysis of differential mRNA and circRNA profiles in primary and metastatic pancreatic neuroendocrine tumors |
| title_full | Comprehensive analysis of differential mRNA and circRNA profiles in primary and metastatic pancreatic neuroendocrine tumors |
| title_fullStr | Comprehensive analysis of differential mRNA and circRNA profiles in primary and metastatic pancreatic neuroendocrine tumors |
| title_full_unstemmed | Comprehensive analysis of differential mRNA and circRNA profiles in primary and metastatic pancreatic neuroendocrine tumors |
| title_short | Comprehensive analysis of differential mRNA and circRNA profiles in primary and metastatic pancreatic neuroendocrine tumors |
| title_sort | comprehensive analysis of differential mrna and circrna profiles in primary and metastatic pancreatic neuroendocrine tumors |
| topic | Primary pancreatic neuroendocrine tumor Live metastasis RNA sequencing CircRNA microarrays Tumor adhesion |
| url | http://www.sciencedirect.com/science/article/pii/S2405580825000226 |
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