A protocol for assessing the adhesion potential of lactic acid bacteria on various cell types
Summary: Lactic acid bacteria (LAB) are a large group produced during carbohydrate fermentation, resulting in lactic acid. Here, we present a protocol for probiotic adhesion and interaction into buccal epithelial cells, chicken epithelial cells, and HT-29 cells, as well as for autoaggregation and co...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725000255 |
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| author | Lakshmi Jayaram Ramith Ramu Deepthi Puttegowda Jayanthi M K Chandana Kumari V B Khang Wen Goh |
| author_facet | Lakshmi Jayaram Ramith Ramu Deepthi Puttegowda Jayanthi M K Chandana Kumari V B Khang Wen Goh |
| author_sort | Lakshmi Jayaram |
| collection | DOAJ |
| description | Summary: Lactic acid bacteria (LAB) are a large group produced during carbohydrate fermentation, resulting in lactic acid. Here, we present a protocol for probiotic adhesion and interaction into buccal epithelial cells, chicken epithelial cells, and HT-29 cells, as well as for autoaggregation and coaggregation. We describe steps for visualizing LAB adhesion by imaging cell adhesion using a light microscope. We then detail procedures for performing an adhesion assay.For complete details on the use and execution of this protocol, please refer to Kumari et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-adc440e168a14095aa4f0d3095b773e8 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-adc440e168a14095aa4f0d3095b773e82025-02-07T04:48:20ZengElsevierSTAR Protocols2666-16672025-03-0161103619A protocol for assessing the adhesion potential of lactic acid bacteria on various cell typesLakshmi Jayaram0Ramith Ramu1Deepthi Puttegowda2Jayanthi M K3Chandana Kumari V B4Khang Wen Goh5Department of Biotechnology and Bioinformatics, JSS Academy of Higher Education & Research, Mysore, Karnataka 570015, IndiaDepartment of Biotechnology and Bioinformatics, JSS Academy of Higher Education & Research, Mysore, Karnataka 570015, India; Corresponding authorDepartment of Biotechnology and Bioinformatics, JSS Academy of Higher Education & Research, Mysore, Karnataka 570015, IndiaDepartment of Pharmacology, JSS Medical College, JSS Academy of Higher Education & Research, Mysore, Karnataka 570015, IndiaDepartment of Biotechnology and Bioinformatics, JSS Academy of Higher Education & Research, Mysore, Karnataka 570015, IndiaFaculty of Data Science and Information Technology, INTI International University, Nilai 71800, MalaysiaSummary: Lactic acid bacteria (LAB) are a large group produced during carbohydrate fermentation, resulting in lactic acid. Here, we present a protocol for probiotic adhesion and interaction into buccal epithelial cells, chicken epithelial cells, and HT-29 cells, as well as for autoaggregation and coaggregation. We describe steps for visualizing LAB adhesion by imaging cell adhesion using a light microscope. We then detail procedures for performing an adhesion assay.For complete details on the use and execution of this protocol, please refer to Kumari et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725000255Cell cultureMicrobiologyMicroscopy |
| spellingShingle | Lakshmi Jayaram Ramith Ramu Deepthi Puttegowda Jayanthi M K Chandana Kumari V B Khang Wen Goh A protocol for assessing the adhesion potential of lactic acid bacteria on various cell types STAR Protocols Cell culture Microbiology Microscopy |
| title | A protocol for assessing the adhesion potential of lactic acid bacteria on various cell types |
| title_full | A protocol for assessing the adhesion potential of lactic acid bacteria on various cell types |
| title_fullStr | A protocol for assessing the adhesion potential of lactic acid bacteria on various cell types |
| title_full_unstemmed | A protocol for assessing the adhesion potential of lactic acid bacteria on various cell types |
| title_short | A protocol for assessing the adhesion potential of lactic acid bacteria on various cell types |
| title_sort | protocol for assessing the adhesion potential of lactic acid bacteria on various cell types |
| topic | Cell culture Microbiology Microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725000255 |
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