Disease resistance traits of IPB-D2 chickens: Characterization of IgY concentrations, Newcastle disease antibody titers, and leukocyte profiles
Background and Aim: The IPB-D2 chicken, a potential disease-resistant local Indonesian line, has been developed to improve poultry health and productivity for small-scale farmers. This study aimed to characterize the disease resistance traits of IPB-D2 chickens by analyzing key immunological paramet...
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Veterinary World
2025-01-01
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| Series: | Veterinary World |
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| Online Access: | https://www.veterinaryworld.org/Vol.18/January-2025/19.pdf |
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| author | Dwi Lestari Sri Murtini Niken Ulupi Asep Gunawan Isyana Khaerunnisa Anneke Anggraeni Cece Sumantri |
| author_facet | Dwi Lestari Sri Murtini Niken Ulupi Asep Gunawan Isyana Khaerunnisa Anneke Anggraeni Cece Sumantri |
| author_sort | Dwi Lestari |
| collection | DOAJ |
| description | Background and Aim: The IPB-D2 chicken, a potential disease-resistant local Indonesian line, has been developed to improve poultry health and productivity for small-scale farmers. This study aimed to characterize the disease resistance traits of IPB-D2 chickens by analyzing key immunological parameters, including immunoglobulin Y (IgY) concentration, Newcastle disease (ND) antibody titers, and leukocyte profiles.
Materials and Methods: A total of 100 IPB-D2 chickens were studied. Blood samples from 21-week-old chickens were collected for analysis of IgY concentrations using indirect enzyme-linked immunosorbent assay, ND antibody titers using the hemagglutination inhibition test, and leukocyte profiling using the Giemsa staining method. Correlations between parameters were determined using Pearson’s correlation analysis in RStudio.
Results: The findings revealed that IPB-D2 chickens exhibited higher IgY concentrations, leukocyte counts, heterophil levels, and monocyte counts compared to their IPB-D1 progenitors. However, ND antibody titers in IPB-D2 chickens were lower than the protective threshold. Significant positive correlations were observed between leukocytes and lymphocytes, and negative correlations were identified between heterophils and lymphocytes.
Conclusion: IPB-D2 chickens demonstrated a well-balanced immune system with enhanced non-specific (leukocyte and heterophil) and specific (IgY) immune responses. These traits highlight their potential as a disease-resistant poultry line. Future research should focus on molecular selection through transcriptomic analysis to identify candidate genes associated with disease resistance traits, enabling further genetic improvements. |
| format | Article |
| id | doaj-art-b25dafb266574143b0bf11a6540bfef2 |
| institution | Kabale University |
| issn | 0972-8988 2231-0916 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj-art-b25dafb266574143b0bf11a6540bfef22025-02-07T12:26:13ZengVeterinary WorldVeterinary World0972-89882231-09162025-01-0118117217710.14202/vetworld.2025.172-177Disease resistance traits of IPB-D2 chickens: Characterization of IgY concentrations, Newcastle disease antibody titers, and leukocyte profilesDwi Lestari0https://orcid.org/0009-0002-5912-4254Sri Murtini1https://orcid.org/0000-0001-6251-8735Niken Ulupi2https://orcid.org/0000-0002-3859-2676Asep Gunawan3https://orcid.org/0000-0003-0468-2443Isyana Khaerunnisa4https://orcid.org/0000-0002-5974-7892Anneke Anggraeni5https://orcid.org/0009-0007-8279-8886Cece Sumantri6https://orcid.org/0000-0002-5729-1254Research Center for Applied Zoology, National Research and Innovation Agency, Bogor, 16911, Indonesia.Division of Medical Microbiology, School of Veterinary Medicine and Biomedical Science, IPB University, Bogor, 16680, Indonesia.Department of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor, 16680, Indonesia.Department of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor, 16680, Indonesia.Research Center for Applied Zoology, National Research and Innovation Agency, Bogor, 16911, Indonesia.Research Center for Animal Husbandry, National Research and Innovation Agency, Bogor, 16911, Indonesia.Department of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor, 16680, Indonesia.Background and Aim: The IPB-D2 chicken, a potential disease-resistant local Indonesian line, has been developed to improve poultry health and productivity for small-scale farmers. This study aimed to characterize the disease resistance traits of IPB-D2 chickens by analyzing key immunological parameters, including immunoglobulin Y (IgY) concentration, Newcastle disease (ND) antibody titers, and leukocyte profiles. Materials and Methods: A total of 100 IPB-D2 chickens were studied. Blood samples from 21-week-old chickens were collected for analysis of IgY concentrations using indirect enzyme-linked immunosorbent assay, ND antibody titers using the hemagglutination inhibition test, and leukocyte profiling using the Giemsa staining method. Correlations between parameters were determined using Pearson’s correlation analysis in RStudio. Results: The findings revealed that IPB-D2 chickens exhibited higher IgY concentrations, leukocyte counts, heterophil levels, and monocyte counts compared to their IPB-D1 progenitors. However, ND antibody titers in IPB-D2 chickens were lower than the protective threshold. Significant positive correlations were observed between leukocytes and lymphocytes, and negative correlations were identified between heterophils and lymphocytes. Conclusion: IPB-D2 chickens demonstrated a well-balanced immune system with enhanced non-specific (leukocyte and heterophil) and specific (IgY) immune responses. These traits highlight their potential as a disease-resistant poultry line. Future research should focus on molecular selection through transcriptomic analysis to identify candidate genes associated with disease resistance traits, enabling further genetic improvements.https://www.veterinaryworld.org/Vol.18/January-2025/19.pdfipb-d2 chickenimmunoglobulin y concentrationnd antibody titerleukocyte profile |
| spellingShingle | Dwi Lestari Sri Murtini Niken Ulupi Asep Gunawan Isyana Khaerunnisa Anneke Anggraeni Cece Sumantri Disease resistance traits of IPB-D2 chickens: Characterization of IgY concentrations, Newcastle disease antibody titers, and leukocyte profiles Veterinary World ipb-d2 chicken immunoglobulin y concentration nd antibody titer leukocyte profile |
| title | Disease resistance traits of IPB-D2 chickens: Characterization of IgY concentrations, Newcastle disease antibody titers, and leukocyte profiles |
| title_full | Disease resistance traits of IPB-D2 chickens: Characterization of IgY concentrations, Newcastle disease antibody titers, and leukocyte profiles |
| title_fullStr | Disease resistance traits of IPB-D2 chickens: Characterization of IgY concentrations, Newcastle disease antibody titers, and leukocyte profiles |
| title_full_unstemmed | Disease resistance traits of IPB-D2 chickens: Characterization of IgY concentrations, Newcastle disease antibody titers, and leukocyte profiles |
| title_short | Disease resistance traits of IPB-D2 chickens: Characterization of IgY concentrations, Newcastle disease antibody titers, and leukocyte profiles |
| title_sort | disease resistance traits of ipb d2 chickens characterization of igy concentrations newcastle disease antibody titers and leukocyte profiles |
| topic | ipb-d2 chicken immunoglobulin y concentration nd antibody titer leukocyte profile |
| url | https://www.veterinaryworld.org/Vol.18/January-2025/19.pdf |
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