Sustainable Ultrasound-Assisted Solid-Phase peptide synthesis (SUS-SPPS): Less Waste, more efficiency
The integration of low-frequency ultrasound with Solid-Phase Peptide Synthesis (SPPS) was explored to establish a Sustainable Ultrasound-assisted Solid-Phase Peptide Synthesis (SUS-SPPS) method. This innovative approach significantly reduces solvent consumption, washing steps, time, and reagent usag...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Ultrasonics Sonochemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417725000367 |
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| author | Salvatore Mottola Alessandra Del Bene Vincenzo Mazzarella Roberto Cutolo Ida Boccino Francesco Merlino Sandro Cosconati Salvatore Di Maro Anna Messere |
| author_facet | Salvatore Mottola Alessandra Del Bene Vincenzo Mazzarella Roberto Cutolo Ida Boccino Francesco Merlino Sandro Cosconati Salvatore Di Maro Anna Messere |
| author_sort | Salvatore Mottola |
| collection | DOAJ |
| description | The integration of low-frequency ultrasound with Solid-Phase Peptide Synthesis (SPPS) was explored to establish a Sustainable Ultrasound-assisted Solid-Phase Peptide Synthesis (SUS-SPPS) method. This innovative approach significantly reduces solvent consumption, washing steps, time, and reagent usage compared to conventional manual SPPS protocols. The SUS-SPPS method exploits ultrasound at every stage of synthesis and work-up, reducing the process to just two steps. The first step sequentially combines Fmoc-amino acid coupling, capping of unreacted amino groups, and Fmoc deprotection into a single operation, while the second one consists of a single washing procedure. Moreover, we demonstrated that the method is compatible with various resin types, including Rink-amide, Wang, and Cl-Trt resins, and facilitates the efficient synthesis of peptides of varying lengths (up to 20-mers) and compositions, including those traditionally considered “difficult sequences”, with excellent yields and purity. Notably, SUS-SPPS reduces solvent usage per coupling cycle by 83–88%, marking a significant breakthrough in sustainable peptide synthesis. |
| format | Article |
| id | doaj-art-b2656b6605a44cd09956a9d08ce76f84 |
| institution | Kabale University |
| issn | 1350-4177 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj-art-b2656b6605a44cd09956a9d08ce76f842025-02-10T04:34:16ZengElsevierUltrasonics Sonochemistry1350-41772025-03-01114107257Sustainable Ultrasound-Assisted Solid-Phase peptide synthesis (SUS-SPPS): Less Waste, more efficiencySalvatore Mottola0Alessandra Del Bene1Vincenzo Mazzarella2Roberto Cutolo3Ida Boccino4Francesco Merlino5Sandro Cosconati6Salvatore Di Maro7Anna Messere8Department of Environmental, Biological and Pharmaceutical Science and Technology, University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyDepartment of Environmental, Biological and Pharmaceutical Science and Technology, University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyDepartment of Environmental, Biological and Pharmaceutical Science and Technology, University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyDepartment of Environmental, Biological and Pharmaceutical Science and Technology, University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyDepartment of Pharmacy, University of Naples Federico II, 80131 Naples, ItalyDepartment of Pharmacy, University of Naples Federico II, 80131 Naples, Italy; Interuniversity Research Centre on Bioactive Peptides (CIRPEB), Naples, ItalyDepartment of Environmental, Biological and Pharmaceutical Science and Technology, University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyDepartment of Environmental, Biological and Pharmaceutical Science and Technology, University of Campania “Luigi Vanvitelli”, 81100 Caserta, Italy; Interuniversity Research Centre on Bioactive Peptides (CIRPEB), Naples, Italy; Corresponding authors at: Department of Environmental, Biological and Pharmaceutical Science and Technology, University of Campania “Luigi Vanvitelli”, 81100 Caserta, Italy.Department of Environmental, Biological and Pharmaceutical Science and Technology, University of Campania “Luigi Vanvitelli”, 81100 Caserta, Italy; Interuniversity Research Centre on Bioactive Peptides (CIRPEB), Naples, Italy; Corresponding authors at: Department of Environmental, Biological and Pharmaceutical Science and Technology, University of Campania “Luigi Vanvitelli”, 81100 Caserta, Italy.The integration of low-frequency ultrasound with Solid-Phase Peptide Synthesis (SPPS) was explored to establish a Sustainable Ultrasound-assisted Solid-Phase Peptide Synthesis (SUS-SPPS) method. This innovative approach significantly reduces solvent consumption, washing steps, time, and reagent usage compared to conventional manual SPPS protocols. The SUS-SPPS method exploits ultrasound at every stage of synthesis and work-up, reducing the process to just two steps. The first step sequentially combines Fmoc-amino acid coupling, capping of unreacted amino groups, and Fmoc deprotection into a single operation, while the second one consists of a single washing procedure. Moreover, we demonstrated that the method is compatible with various resin types, including Rink-amide, Wang, and Cl-Trt resins, and facilitates the efficient synthesis of peptides of varying lengths (up to 20-mers) and compositions, including those traditionally considered “difficult sequences”, with excellent yields and purity. Notably, SUS-SPPS reduces solvent usage per coupling cycle by 83–88%, marking a significant breakthrough in sustainable peptide synthesis.http://www.sciencedirect.com/science/article/pii/S1350417725000367Sustainable Peptide SynthesisUltrasoundSonochemistrySolid-Phase SynthesisMinimized solvent consumptionGreen approach |
| spellingShingle | Salvatore Mottola Alessandra Del Bene Vincenzo Mazzarella Roberto Cutolo Ida Boccino Francesco Merlino Sandro Cosconati Salvatore Di Maro Anna Messere Sustainable Ultrasound-Assisted Solid-Phase peptide synthesis (SUS-SPPS): Less Waste, more efficiency Ultrasonics Sonochemistry Sustainable Peptide Synthesis Ultrasound Sonochemistry Solid-Phase Synthesis Minimized solvent consumption Green approach |
| title | Sustainable Ultrasound-Assisted Solid-Phase peptide synthesis (SUS-SPPS): Less Waste, more efficiency |
| title_full | Sustainable Ultrasound-Assisted Solid-Phase peptide synthesis (SUS-SPPS): Less Waste, more efficiency |
| title_fullStr | Sustainable Ultrasound-Assisted Solid-Phase peptide synthesis (SUS-SPPS): Less Waste, more efficiency |
| title_full_unstemmed | Sustainable Ultrasound-Assisted Solid-Phase peptide synthesis (SUS-SPPS): Less Waste, more efficiency |
| title_short | Sustainable Ultrasound-Assisted Solid-Phase peptide synthesis (SUS-SPPS): Less Waste, more efficiency |
| title_sort | sustainable ultrasound assisted solid phase peptide synthesis sus spps less waste more efficiency |
| topic | Sustainable Peptide Synthesis Ultrasound Sonochemistry Solid-Phase Synthesis Minimized solvent consumption Green approach |
| url | http://www.sciencedirect.com/science/article/pii/S1350417725000367 |
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