The transcriptional response to yellow and wilt disease, caused by race 6 of Fusarium oxysporum f. sp. Ciceris in two contrasting chickpea cultivars
Abstract Background Chickpea (Cicer arietinum L.) ranks as the third most crucial grain legume worldwide. Fusarium wilt (Fusarium oxysporum f. sp. ciceri (Foc)) is a devastating fungal disease that prevents the maximum potential for chickpea production. Results To identify genes and pathways involve...
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BMC
2025-02-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-025-11308-3 |
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| author | Aliakbar Faramarzpour Sara Dezhsetan Hamid Hassaneian Khoshro Raheleh Mirdar Mansuri Hamid Reza Pouralibaba Zahra-Sadat Shobbar |
| author_facet | Aliakbar Faramarzpour Sara Dezhsetan Hamid Hassaneian Khoshro Raheleh Mirdar Mansuri Hamid Reza Pouralibaba Zahra-Sadat Shobbar |
| author_sort | Aliakbar Faramarzpour |
| collection | DOAJ |
| description | Abstract Background Chickpea (Cicer arietinum L.) ranks as the third most crucial grain legume worldwide. Fusarium wilt (Fusarium oxysporum f. sp. ciceri (Foc)) is a devastating fungal disease that prevents the maximum potential for chickpea production. Results To identify genes and pathways involved in resistance to race 6 of Foc, this study utilized transcriptome sequencing of two chickpea cultivars: resistant (Ana) and susceptible (Hashem) to Foc race 6. Illumina sequencing of the root samples yielded 133.5 million raw reads, with about 90% of the clean reads mapped to the chickpea reference genome. The analysis revealed that 548 genes (332 upregulated and 216 downregulated) in the resistant genotype (Ana) and 1115 genes (595 upregulated and 520 downregulated) in the susceptible genotype (Hashem) were differentially expressed under Fusarium wilt (FW) disease stress caused by Foc race 6. The expression patterns of some differentially expressed genes (DEGs) were validated using quantitative real-time PCR. A total of 131 genes were exclusively upregulated under FW stress in the resistant cultivar, including several genes involved in sensing (e.g., CaNLR-RPM1, CaLYK5-RLK, CaPR5-RLK, CaLRR-RLK, and CaRLP-EIX2), signaling (e.g., CaPP7, CaEPS1, CaSTY13, and CaPR-1), transcription regulation (e.g., CaMYBs, CaGLK, CaERFs, CaZAT11-like, and CaNAC6) and cell wall integrity (e.g., CaPGI2-like, CaEXLs, CaCSLD and CaCYP73A100-like). Conclusions The achieved results could provide insights into the molecular mechanism underlying resistance to FW and could be valuable for breeding programs aimed at developing FW-resistant chickpea varieties. |
| format | Article |
| id | doaj-art-b3265218df5143e9a4175998177d5340 |
| institution | Kabale University |
| issn | 1471-2164 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj-art-b3265218df5143e9a4175998177d53402025-02-09T12:13:55ZengBMCBMC Genomics1471-21642025-02-0126111710.1186/s12864-025-11308-3The transcriptional response to yellow and wilt disease, caused by race 6 of Fusarium oxysporum f. sp. Ciceris in two contrasting chickpea cultivarsAliakbar Faramarzpour0Sara Dezhsetan1Hamid Hassaneian Khoshro2Raheleh Mirdar Mansuri3Hamid Reza Pouralibaba4Zahra-Sadat Shobbar5Department of Plant Production & Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh ArdabiliDepartment of Plant Production & Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh ArdabiliDryland Agricultural Research Institute (DARI), Agriculture Research, Education and Extension Organization (AREEO)Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO)Dryland Agricultural Research Institute (DARI), Agriculture Research, Education and Extension Organization (AREEO)Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO)Abstract Background Chickpea (Cicer arietinum L.) ranks as the third most crucial grain legume worldwide. Fusarium wilt (Fusarium oxysporum f. sp. ciceri (Foc)) is a devastating fungal disease that prevents the maximum potential for chickpea production. Results To identify genes and pathways involved in resistance to race 6 of Foc, this study utilized transcriptome sequencing of two chickpea cultivars: resistant (Ana) and susceptible (Hashem) to Foc race 6. Illumina sequencing of the root samples yielded 133.5 million raw reads, with about 90% of the clean reads mapped to the chickpea reference genome. The analysis revealed that 548 genes (332 upregulated and 216 downregulated) in the resistant genotype (Ana) and 1115 genes (595 upregulated and 520 downregulated) in the susceptible genotype (Hashem) were differentially expressed under Fusarium wilt (FW) disease stress caused by Foc race 6. The expression patterns of some differentially expressed genes (DEGs) were validated using quantitative real-time PCR. A total of 131 genes were exclusively upregulated under FW stress in the resistant cultivar, including several genes involved in sensing (e.g., CaNLR-RPM1, CaLYK5-RLK, CaPR5-RLK, CaLRR-RLK, and CaRLP-EIX2), signaling (e.g., CaPP7, CaEPS1, CaSTY13, and CaPR-1), transcription regulation (e.g., CaMYBs, CaGLK, CaERFs, CaZAT11-like, and CaNAC6) and cell wall integrity (e.g., CaPGI2-like, CaEXLs, CaCSLD and CaCYP73A100-like). Conclusions The achieved results could provide insights into the molecular mechanism underlying resistance to FW and could be valuable for breeding programs aimed at developing FW-resistant chickpea varieties.https://doi.org/10.1186/s12864-025-11308-3ChickpeaBiotic stressFusarium wilt (race 6)RNA sequencing |
| spellingShingle | Aliakbar Faramarzpour Sara Dezhsetan Hamid Hassaneian Khoshro Raheleh Mirdar Mansuri Hamid Reza Pouralibaba Zahra-Sadat Shobbar The transcriptional response to yellow and wilt disease, caused by race 6 of Fusarium oxysporum f. sp. Ciceris in two contrasting chickpea cultivars BMC Genomics Chickpea Biotic stress Fusarium wilt (race 6) RNA sequencing |
| title | The transcriptional response to yellow and wilt disease, caused by race 6 of Fusarium oxysporum f. sp. Ciceris in two contrasting chickpea cultivars |
| title_full | The transcriptional response to yellow and wilt disease, caused by race 6 of Fusarium oxysporum f. sp. Ciceris in two contrasting chickpea cultivars |
| title_fullStr | The transcriptional response to yellow and wilt disease, caused by race 6 of Fusarium oxysporum f. sp. Ciceris in two contrasting chickpea cultivars |
| title_full_unstemmed | The transcriptional response to yellow and wilt disease, caused by race 6 of Fusarium oxysporum f. sp. Ciceris in two contrasting chickpea cultivars |
| title_short | The transcriptional response to yellow and wilt disease, caused by race 6 of Fusarium oxysporum f. sp. Ciceris in two contrasting chickpea cultivars |
| title_sort | transcriptional response to yellow and wilt disease caused by race 6 of fusarium oxysporum f sp ciceris in two contrasting chickpea cultivars |
| topic | Chickpea Biotic stress Fusarium wilt (race 6) RNA sequencing |
| url | https://doi.org/10.1186/s12864-025-11308-3 |
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