The Effect of Exosomes Derived from Human Umbilical Cord Mesenchymal Stromal/Stem Cells on the Regeneration of Human Pulpectomized Tooth: A Case Report
The dental pulp is essential for tooth health and sensory function. However, conventional treatments for infected or necrotic pulp, such as root canal therapy, frequently result in tooth loss and increased fracture risk. Regenerative endodontic procedures (REPs) aim to regenerate pulp tissue while p...
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Shiraz University of Medical Sciences
2025-01-01
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| Series: | Iranian Journal of Medical Sciences |
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| Online Access: | https://ijms.sums.ac.ir/article_50602_de508c4eaf45e9c3bcac0fd5ba4bb4c8.pdf |
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| author | Nazanin Jafari Mina Seyed Habashi Alireza Afshar Mohammad Ghasemi Madina A. Kurmanalina Nadiar M. Mussin Asset A. Kaliyev Shahrokh Zare Zahra Khodabandeh Nader Tanideh Amin Tamadon |
| author_facet | Nazanin Jafari Mina Seyed Habashi Alireza Afshar Mohammad Ghasemi Madina A. Kurmanalina Nadiar M. Mussin Asset A. Kaliyev Shahrokh Zare Zahra Khodabandeh Nader Tanideh Amin Tamadon |
| author_sort | Nazanin Jafari |
| collection | DOAJ |
| description | The dental pulp is essential for tooth health and sensory function. However, conventional treatments for infected or necrotic pulp, such as root canal therapy, frequently result in tooth loss and increased fracture risk. Regenerative endodontic procedures (REPs) aim to regenerate pulp tissue while preserving tooth vitality. Exosomes from human umbilical cord mesenchymal stromal/stem cells (hUCMSCs) demonstrated potential for tissue regeneration. In this case report, the potential of hUCMSC-derived exosomes for regenerating the dental pulp of a pulpectomized tooth was investigated. The patient, a 40-year-old man, was presented with irreversible pulpitis in the mandibular second premolar. The patient underwent a pulpectomy, followed by the application of a chitosan and hUCMSC-derived exosome mixture into the root canal. This project was conceived and executed as a joint project in Bushehr (Iran), Shiraz (Iran), and Aktobe (Kazakhstan) from 2022 to 2024. Exosomes were isolated and characterized using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Clinical evaluations, including visual inspection, palpation, pulp vitality tests, radiography, and cone beam computed tomography (CBCT) imaging, were conducted over 24 weeks post-treatment. The SEM and TEM images confirmed the effective isolation and characterization of exosomes. Clinical follow-ups showed no signs of infection, swelling, or tenderness around the treated tooth. Radiographic assessments indicated periapical radiolucency and periodontal ligament widening, suggesting active healing. Despite these radiographic changes, the absence of clinical symptoms indicated successful tissue regeneration and integration. This case report demonstrated the potential of hUCMSC-derived exosomes for dental pulp regeneration, with promising results in maintaining tooth vitality and promoting healing. |
| format | Article |
| id | doaj-art-b3c6cb7ba0e74e3da2a286c62d4fb89d |
| institution | Kabale University |
| issn | 0253-0716 1735-3688 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Shiraz University of Medical Sciences |
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| series | Iranian Journal of Medical Sciences |
| spelling | doaj-art-b3c6cb7ba0e74e3da2a286c62d4fb89d2025-02-11T07:30:14ZengShiraz University of Medical SciencesIranian Journal of Medical Sciences0253-07161735-36882025-01-01501545810.30476/ijms.2024.103117.363850602The Effect of Exosomes Derived from Human Umbilical Cord Mesenchymal Stromal/Stem Cells on the Regeneration of Human Pulpectomized Tooth: A Case ReportNazanin Jafari0Mina Seyed Habashi1Alireza Afshar2Mohammad Ghasemi3Madina A. Kurmanalina4Nadiar M. Mussin5Asset A. Kaliyev6Shahrokh Zare7Zahra Khodabandeh8Nader Tanideh9Amin Tamadon10School of Dentistry, Bushehr University of Medical Sciences, Bushehr, IranDepartment of Endodontics, School of Dentistry, Bushehr University of Medical Sciences, Bushehr, IranStudent Research Committee, Bushehr University of Medical Sciences, Bushehr, IranDepartment of Radiology, School of Dentistry, Bushehr University of Medical Sciences, Bushehr, IranDepartment of Therapeutic and Prosthetic Dentistry, West Kazakhstan Marat Ospanov Medical University, Aktobe, KazakhstanDepartment of Surgery No. 2, West Kazakhstan Marat Ospanov Medical University Aktobe, KazakhstanDepartment of Surgery No. 2, West Kazakhstan Marat Ospanov Medical University Aktobe, KazakhstanStem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IranStem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IranPerciaVista R&D Co. Shiraz, IranPerciaVista R&D Co. Shiraz, IranThe dental pulp is essential for tooth health and sensory function. However, conventional treatments for infected or necrotic pulp, such as root canal therapy, frequently result in tooth loss and increased fracture risk. Regenerative endodontic procedures (REPs) aim to regenerate pulp tissue while preserving tooth vitality. Exosomes from human umbilical cord mesenchymal stromal/stem cells (hUCMSCs) demonstrated potential for tissue regeneration. In this case report, the potential of hUCMSC-derived exosomes for regenerating the dental pulp of a pulpectomized tooth was investigated. The patient, a 40-year-old man, was presented with irreversible pulpitis in the mandibular second premolar. The patient underwent a pulpectomy, followed by the application of a chitosan and hUCMSC-derived exosome mixture into the root canal. This project was conceived and executed as a joint project in Bushehr (Iran), Shiraz (Iran), and Aktobe (Kazakhstan) from 2022 to 2024. Exosomes were isolated and characterized using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Clinical evaluations, including visual inspection, palpation, pulp vitality tests, radiography, and cone beam computed tomography (CBCT) imaging, were conducted over 24 weeks post-treatment. The SEM and TEM images confirmed the effective isolation and characterization of exosomes. Clinical follow-ups showed no signs of infection, swelling, or tenderness around the treated tooth. Radiographic assessments indicated periapical radiolucency and periodontal ligament widening, suggesting active healing. Despite these radiographic changes, the absence of clinical symptoms indicated successful tissue regeneration and integration. This case report demonstrated the potential of hUCMSC-derived exosomes for dental pulp regeneration, with promising results in maintaining tooth vitality and promoting healing.https://ijms.sums.ac.ir/article_50602_de508c4eaf45e9c3bcac0fd5ba4bb4c8.pdfexosomesregenerative endodonticsdental pulpregenerationmesenchymal stem cells |
| spellingShingle | Nazanin Jafari Mina Seyed Habashi Alireza Afshar Mohammad Ghasemi Madina A. Kurmanalina Nadiar M. Mussin Asset A. Kaliyev Shahrokh Zare Zahra Khodabandeh Nader Tanideh Amin Tamadon The Effect of Exosomes Derived from Human Umbilical Cord Mesenchymal Stromal/Stem Cells on the Regeneration of Human Pulpectomized Tooth: A Case Report Iranian Journal of Medical Sciences exosomes regenerative endodontics dental pulp regeneration mesenchymal stem cells |
| title | The Effect of Exosomes Derived from Human Umbilical Cord Mesenchymal Stromal/Stem Cells on the Regeneration of Human Pulpectomized Tooth: A Case Report |
| title_full | The Effect of Exosomes Derived from Human Umbilical Cord Mesenchymal Stromal/Stem Cells on the Regeneration of Human Pulpectomized Tooth: A Case Report |
| title_fullStr | The Effect of Exosomes Derived from Human Umbilical Cord Mesenchymal Stromal/Stem Cells on the Regeneration of Human Pulpectomized Tooth: A Case Report |
| title_full_unstemmed | The Effect of Exosomes Derived from Human Umbilical Cord Mesenchymal Stromal/Stem Cells on the Regeneration of Human Pulpectomized Tooth: A Case Report |
| title_short | The Effect of Exosomes Derived from Human Umbilical Cord Mesenchymal Stromal/Stem Cells on the Regeneration of Human Pulpectomized Tooth: A Case Report |
| title_sort | effect of exosomes derived from human umbilical cord mesenchymal stromal stem cells on the regeneration of human pulpectomized tooth a case report |
| topic | exosomes regenerative endodontics dental pulp regeneration mesenchymal stem cells |
| url | https://ijms.sums.ac.ir/article_50602_de508c4eaf45e9c3bcac0fd5ba4bb4c8.pdf |
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