Identification and biological characterization of the pathogen responsible for acute haemorrhagic disease in largemouth bass
To investigate the cause of mass mortality in largemouth bass (Micropterus salmoides) at an aquaculture farm in Hubei due to acute hemorrhagic disease, a dominant bacterium named LY02 was isolated from the brain and major internal organs of diseased fishdise. A re-infection experiment confirmed that...
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| Language: | English |
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424006422 |
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| author | Yibin Yang Ping Deng Hao He Qiuhong Yang Xiaohui Ai Xia Zhu |
| author_facet | Yibin Yang Ping Deng Hao He Qiuhong Yang Xiaohui Ai Xia Zhu |
| author_sort | Yibin Yang |
| collection | DOAJ |
| description | To investigate the cause of mass mortality in largemouth bass (Micropterus salmoides) at an aquaculture farm in Hubei due to acute hemorrhagic disease, a dominant bacterium named LY02 was isolated from the brain and major internal organs of diseased fishdise. A re-infection experiment confirmed that LY02 was the pathogenic bacterium responsible for the mass mortality. Histopathological studies revealed that the infection caused varying degrees of necrosis and congestion in the brain and major internal organs of the largemouth bass. Polymerase chain reaction (PCR) detection showed that the LY02 isolate carried the toxin genes simA, pdi, sagA, and cfi. Whole-genome analysis identified LY02 as Streptococcus iniae, carrying 330 toxin factor-related genes and 164 resistance factor-related genes. LY02 was found to be highly sensitive to ceftizoxime, cefradine, cefotaxime, azithromycin, erythromycin, enoxacin, doxycycline, and netilmicin; moderately sensitive to norfloxacin and enrofloxacin; and resistant to gentamicin, streptomycin, kanamycin, tobramycin, and neomycin. This study is the first to isolate and identify S. iniae from infected largemouth bass, providing a theoretical basis for the diagnosis and prevention of acute hemorrhagic disease in this species. |
| format | Article |
| id | doaj-art-b588106661d743a9a7dd025c8c4990d5 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-b588106661d743a9a7dd025c8c4990d52025-02-06T05:12:07ZengElsevierAquaculture Reports2352-51342025-03-0140102554Identification and biological characterization of the pathogen responsible for acute haemorrhagic disease in largemouth bassYibin Yang0Ping Deng1Hao He2Qiuhong Yang3Xiaohui Ai4Xia Zhu5Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; Corresponding authors.Wuhan Academy of Agricultural Sciences, Wuhan 430064, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; Corresponding authors.Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; Corresponding authors.To investigate the cause of mass mortality in largemouth bass (Micropterus salmoides) at an aquaculture farm in Hubei due to acute hemorrhagic disease, a dominant bacterium named LY02 was isolated from the brain and major internal organs of diseased fishdise. A re-infection experiment confirmed that LY02 was the pathogenic bacterium responsible for the mass mortality. Histopathological studies revealed that the infection caused varying degrees of necrosis and congestion in the brain and major internal organs of the largemouth bass. Polymerase chain reaction (PCR) detection showed that the LY02 isolate carried the toxin genes simA, pdi, sagA, and cfi. Whole-genome analysis identified LY02 as Streptococcus iniae, carrying 330 toxin factor-related genes and 164 resistance factor-related genes. LY02 was found to be highly sensitive to ceftizoxime, cefradine, cefotaxime, azithromycin, erythromycin, enoxacin, doxycycline, and netilmicin; moderately sensitive to norfloxacin and enrofloxacin; and resistant to gentamicin, streptomycin, kanamycin, tobramycin, and neomycin. This study is the first to isolate and identify S. iniae from infected largemouth bass, providing a theoretical basis for the diagnosis and prevention of acute hemorrhagic disease in this species.http://www.sciencedirect.com/science/article/pii/S2352513424006422Streptococcus iniaeLargemouth bassWhole genomeVirulenceDrug resistance |
| spellingShingle | Yibin Yang Ping Deng Hao He Qiuhong Yang Xiaohui Ai Xia Zhu Identification and biological characterization of the pathogen responsible for acute haemorrhagic disease in largemouth bass Aquaculture Reports Streptococcus iniae Largemouth bass Whole genome Virulence Drug resistance |
| title | Identification and biological characterization of the pathogen responsible for acute haemorrhagic disease in largemouth bass |
| title_full | Identification and biological characterization of the pathogen responsible for acute haemorrhagic disease in largemouth bass |
| title_fullStr | Identification and biological characterization of the pathogen responsible for acute haemorrhagic disease in largemouth bass |
| title_full_unstemmed | Identification and biological characterization of the pathogen responsible for acute haemorrhagic disease in largemouth bass |
| title_short | Identification and biological characterization of the pathogen responsible for acute haemorrhagic disease in largemouth bass |
| title_sort | identification and biological characterization of the pathogen responsible for acute haemorrhagic disease in largemouth bass |
| topic | Streptococcus iniae Largemouth bass Whole genome Virulence Drug resistance |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424006422 |
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