Structural characteristics, sugar metabolizing enzyme activity and biological activity of Ganoderma lucidum polysaccharides at different growth stages
Abstract The Ganoderma lucidum polysaccharides (GLPs) content, monosaccharide composition, molecular weight, sugar metabolism-related enzyme activities and in vitro antioxidant activities of Ganoderma lucidum fruiting body (GLFB) from cassava stalk cultivation at different growth stages (namely, dif...
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Nature Portfolio
2025-02-01
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| Online Access: | https://doi.org/10.1038/s41598-025-89559-z |
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| author | Shengjian Ma Yuliang Chen Huaguo Huang Xiaorui Pu Huangbin Liang Yu Kuang Yijun Liu |
| author_facet | Shengjian Ma Yuliang Chen Huaguo Huang Xiaorui Pu Huangbin Liang Yu Kuang Yijun Liu |
| author_sort | Shengjian Ma |
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| description | Abstract The Ganoderma lucidum polysaccharides (GLPs) content, monosaccharide composition, molecular weight, sugar metabolism-related enzyme activities and in vitro antioxidant activities of Ganoderma lucidum fruiting body (GLFB) from cassava stalk cultivation at different growth stages (namely, differentiation stage, umbrella stage, maturity stage and spore stage) were determined. The results showed that the monosaccharides of GLPs in GLFB were mainly composed of galactose, glucose, and mannose. During the maturation of GLFB, the polysaccharide content, hexose kinase and phosphoglucose isomerase activities showed an increasing trend, the structure of functional groups did not change significantly, and the molecular weight of GLPs showed a tendency to increase (112 kDa up to 11358 kDa) and then decrease (11358 kDa down to 7678 kDa). Of the four growth stages, the spore stage had the highest GLPs content of 4.11%, the highest hexokinase (HK) and phosphoglucose isomerase (PGM) activities of 4051.4 and 322.1 nmol/(min/g), respectively, the highest total antioxidant activity (18.79 mmol/mL) and DPPH radical scavenging capacity (36.15%). This study provided a theoretical basis for the application of cassava stalks in GLFB cultivation and harvesting. |
| format | Article |
| id | doaj-art-b6a6ef75b510472d83db89b5c5c1bf02 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-b6a6ef75b510472d83db89b5c5c1bf022025-02-09T12:31:57ZengNature PortfolioScientific Reports2045-23222025-02-0115111110.1038/s41598-025-89559-zStructural characteristics, sugar metabolizing enzyme activity and biological activity of Ganoderma lucidum polysaccharides at different growth stagesShengjian Ma0Yuliang Chen1Huaguo Huang2Xiaorui Pu3Huangbin Liang4Yu Kuang5Yijun Liu6College of Life Science and Technology, Lingnan Normal UniversityHainan Key Laboratory of Storage & Processing of Fruits and Vegetables, Agricultural Products Processing Research Institute, Chinese Academy of Tropical Agricultural SciencesZhejiang Yipu Inspection & Testing Technology Service Co., LtdHainan Key Laboratory of Storage & Processing of Fruits and Vegetables, Agricultural Products Processing Research Institute, Chinese Academy of Tropical Agricultural SciencesHainan Key Laboratory of Storage & Processing of Fruits and Vegetables, Agricultural Products Processing Research Institute, Chinese Academy of Tropical Agricultural SciencesYunnan Dehong Insititution of Tropical Agricultural ScienceHainan Key Laboratory of Storage & Processing of Fruits and Vegetables, Agricultural Products Processing Research Institute, Chinese Academy of Tropical Agricultural SciencesAbstract The Ganoderma lucidum polysaccharides (GLPs) content, monosaccharide composition, molecular weight, sugar metabolism-related enzyme activities and in vitro antioxidant activities of Ganoderma lucidum fruiting body (GLFB) from cassava stalk cultivation at different growth stages (namely, differentiation stage, umbrella stage, maturity stage and spore stage) were determined. The results showed that the monosaccharides of GLPs in GLFB were mainly composed of galactose, glucose, and mannose. During the maturation of GLFB, the polysaccharide content, hexose kinase and phosphoglucose isomerase activities showed an increasing trend, the structure of functional groups did not change significantly, and the molecular weight of GLPs showed a tendency to increase (112 kDa up to 11358 kDa) and then decrease (11358 kDa down to 7678 kDa). Of the four growth stages, the spore stage had the highest GLPs content of 4.11%, the highest hexokinase (HK) and phosphoglucose isomerase (PGM) activities of 4051.4 and 322.1 nmol/(min/g), respectively, the highest total antioxidant activity (18.79 mmol/mL) and DPPH radical scavenging capacity (36.15%). This study provided a theoretical basis for the application of cassava stalks in GLFB cultivation and harvesting.https://doi.org/10.1038/s41598-025-89559-zGanoderma lucidumCassava stalksPolysaccharidesEnzymatic activityBioactivity |
| spellingShingle | Shengjian Ma Yuliang Chen Huaguo Huang Xiaorui Pu Huangbin Liang Yu Kuang Yijun Liu Structural characteristics, sugar metabolizing enzyme activity and biological activity of Ganoderma lucidum polysaccharides at different growth stages Scientific Reports Ganoderma lucidum Cassava stalks Polysaccharides Enzymatic activity Bioactivity |
| title | Structural characteristics, sugar metabolizing enzyme activity and biological activity of Ganoderma lucidum polysaccharides at different growth stages |
| title_full | Structural characteristics, sugar metabolizing enzyme activity and biological activity of Ganoderma lucidum polysaccharides at different growth stages |
| title_fullStr | Structural characteristics, sugar metabolizing enzyme activity and biological activity of Ganoderma lucidum polysaccharides at different growth stages |
| title_full_unstemmed | Structural characteristics, sugar metabolizing enzyme activity and biological activity of Ganoderma lucidum polysaccharides at different growth stages |
| title_short | Structural characteristics, sugar metabolizing enzyme activity and biological activity of Ganoderma lucidum polysaccharides at different growth stages |
| title_sort | structural characteristics sugar metabolizing enzyme activity and biological activity of ganoderma lucidum polysaccharides at different growth stages |
| topic | Ganoderma lucidum Cassava stalks Polysaccharides Enzymatic activity Bioactivity |
| url | https://doi.org/10.1038/s41598-025-89559-z |
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