Comparative in-vitro degradation of hyaluronic acids exposed to different hyaluronidase enzymes
Objectives: This study aimed to compare the in-vitro behavior of four Hyaluronic acid (HA) gels when exposed to two different hyaluronidase (HSE) formulations. Methods: Four commercially available HA were used: Lyft (Lt; Restylane, Galderma, Sweden), Voluma (Vol; Allergan, AbbVie, USA), UltraDeep (U...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Journal of Oral Biology and Craniofacial Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221242682500003X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823864428853985280 |
|---|---|
| author | Marcelo Germani Márcia V.G.B. de Queiroz Mariana Yuri de França Shimizu Thiago Gomes Teixeira Victor Rogerio Gabriela Giro Victor R.M. Munoz-Lora |
| author_facet | Marcelo Germani Márcia V.G.B. de Queiroz Mariana Yuri de França Shimizu Thiago Gomes Teixeira Victor Rogerio Gabriela Giro Victor R.M. Munoz-Lora |
| author_sort | Marcelo Germani |
| collection | DOAJ |
| description | Objectives: This study aimed to compare the in-vitro behavior of four Hyaluronic acid (HA) gels when exposed to two different hyaluronidase (HSE) formulations. Methods: Four commercially available HA were used: Lyft (Lt; Restylane, Galderma, Sweden), Voluma (Vol; Allergan, AbbVie, USA), UltraDeep (UDe; Rennova, Innovapharma, Brazil), and Subskin (Skn; Perfectha, Sinclair, France). The gels were divided into two groups, with seven aliquots (0.1 mL per aliquot) for each group deposited on a glass plate. A millimeter ruler was positioned behind the plate to measure the gel height. Each aliquot received 100 UTR of one of two HSE formulations. After 2 min, each aliquot was mixed using a 22G needle and left to sit for additional 2 min. Results: Partial and final degradation of the products were obtained through photographic analysis. The final percentage of degradation exhibited significant differences among HA gels (P < 0.001). The results revealed higher degradation in Lt, moderate degradation in UDe, and lower degradation in Vol and Skn. Comparisons of HSE formulations showed no significant differences among them (P = 0.881). Moreover, there was a noticeable degradation after mixing (P < 0.001). Conclusions: Within the limits of this study, it can be suggested that Lt exhibits the higher degradation among the experimented gels. Furthermore, differences among HSE formulations do not appear to significantly impact HA degradation, while the mixing movement of HSE and HA seems to influence the degradation rate. These findings may help guide clinical decisions regarding the use of hyaluronidase in managing HA filler complications or adjustments. |
| format | Article |
| id | doaj-art-b78afc6d5bf948da985431c26707adc9 |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-b78afc6d5bf948da985431c26707adc92025-02-09T05:00:15ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682025-01-01151178182Comparative in-vitro degradation of hyaluronic acids exposed to different hyaluronidase enzymesMarcelo Germani0Márcia V.G.B. de Queiroz1Mariana Yuri de França Shimizu2Thiago Gomes Teixeira3Victor Rogerio4Gabriela Giro5Victor R.M. Munoz-Lora6Department of Periodontology and Implantology, University of Guarulhos, Sao Paulo, BrazilPrivate Office, São Paulo, BrazilLet's HOF Academy, São Paulo, BrazilLet's HOF Academy, São Paulo, BrazilLet's HOF Academy, São Paulo, BrazilDepartment of Periodontology and Implantology, University of Guarulhos, Sao Paulo, BrazilDepartment of Periodontology and Implantology, University of Guarulhos, Sao Paulo, Brazil; Let's HOF Academy, São Paulo, Brazil; Corresponding author: Department of periodontology and implantology, University of Guarulhos, Sao Paulo, Brazil.Objectives: This study aimed to compare the in-vitro behavior of four Hyaluronic acid (HA) gels when exposed to two different hyaluronidase (HSE) formulations. Methods: Four commercially available HA were used: Lyft (Lt; Restylane, Galderma, Sweden), Voluma (Vol; Allergan, AbbVie, USA), UltraDeep (UDe; Rennova, Innovapharma, Brazil), and Subskin (Skn; Perfectha, Sinclair, France). The gels were divided into two groups, with seven aliquots (0.1 mL per aliquot) for each group deposited on a glass plate. A millimeter ruler was positioned behind the plate to measure the gel height. Each aliquot received 100 UTR of one of two HSE formulations. After 2 min, each aliquot was mixed using a 22G needle and left to sit for additional 2 min. Results: Partial and final degradation of the products were obtained through photographic analysis. The final percentage of degradation exhibited significant differences among HA gels (P < 0.001). The results revealed higher degradation in Lt, moderate degradation in UDe, and lower degradation in Vol and Skn. Comparisons of HSE formulations showed no significant differences among them (P = 0.881). Moreover, there was a noticeable degradation after mixing (P < 0.001). Conclusions: Within the limits of this study, it can be suggested that Lt exhibits the higher degradation among the experimented gels. Furthermore, differences among HSE formulations do not appear to significantly impact HA degradation, while the mixing movement of HSE and HA seems to influence the degradation rate. These findings may help guide clinical decisions regarding the use of hyaluronidase in managing HA filler complications or adjustments.http://www.sciencedirect.com/science/article/pii/S221242682500003XHyaluronic acidHyaluronidaseDegradationFillers |
| spellingShingle | Marcelo Germani Márcia V.G.B. de Queiroz Mariana Yuri de França Shimizu Thiago Gomes Teixeira Victor Rogerio Gabriela Giro Victor R.M. Munoz-Lora Comparative in-vitro degradation of hyaluronic acids exposed to different hyaluronidase enzymes Journal of Oral Biology and Craniofacial Research Hyaluronic acid Hyaluronidase Degradation Fillers |
| title | Comparative in-vitro degradation of hyaluronic acids exposed to different hyaluronidase enzymes |
| title_full | Comparative in-vitro degradation of hyaluronic acids exposed to different hyaluronidase enzymes |
| title_fullStr | Comparative in-vitro degradation of hyaluronic acids exposed to different hyaluronidase enzymes |
| title_full_unstemmed | Comparative in-vitro degradation of hyaluronic acids exposed to different hyaluronidase enzymes |
| title_short | Comparative in-vitro degradation of hyaluronic acids exposed to different hyaluronidase enzymes |
| title_sort | comparative in vitro degradation of hyaluronic acids exposed to different hyaluronidase enzymes |
| topic | Hyaluronic acid Hyaluronidase Degradation Fillers |
| url | http://www.sciencedirect.com/science/article/pii/S221242682500003X |
| work_keys_str_mv | AT marcelogermani comparativeinvitrodegradationofhyaluronicacidsexposedtodifferenthyaluronidaseenzymes AT marciavgbdequeiroz comparativeinvitrodegradationofhyaluronicacidsexposedtodifferenthyaluronidaseenzymes AT marianayuridefrancashimizu comparativeinvitrodegradationofhyaluronicacidsexposedtodifferenthyaluronidaseenzymes AT thiagogomesteixeira comparativeinvitrodegradationofhyaluronicacidsexposedtodifferenthyaluronidaseenzymes AT victorrogerio comparativeinvitrodegradationofhyaluronicacidsexposedtodifferenthyaluronidaseenzymes AT gabrielagiro comparativeinvitrodegradationofhyaluronicacidsexposedtodifferenthyaluronidaseenzymes AT victorrmmunozlora comparativeinvitrodegradationofhyaluronicacidsexposedtodifferenthyaluronidaseenzymes |