Nano-delivery platform with strong protection and efficient delivery: preparation of self-assembled RNA pesticide with dual RNAi targets against Apolygus lucorum
Abstract Background RNA pesticide is regarded as the “third revolution in the history of pesticides”. However, the double-stranded RNA (dsRNA) is easily degraded in the environment, and its delivery efficiency is not sufficient for pest management. This study aimed to construct a star polycation (SP...
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BMC
2025-02-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-025-03155-x |
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| author | Heng Qiao Qinhong Jiang Jing Zhao Liubin Xiao Keyan Zhu-Salzman Dejin Xu Guangchun Xu Jie Shen Aiguo Gu Dejun Hao Shuo Yan Yongan Tan |
| author_facet | Heng Qiao Qinhong Jiang Jing Zhao Liubin Xiao Keyan Zhu-Salzman Dejin Xu Guangchun Xu Jie Shen Aiguo Gu Dejun Hao Shuo Yan Yongan Tan |
| author_sort | Heng Qiao |
| collection | DOAJ |
| description | Abstract Background RNA pesticide is regarded as the “third revolution in the history of pesticides”. However, the double-stranded RNA (dsRNA) is easily degraded in the environment, and its delivery efficiency is not sufficient for pest management. This study aimed to construct a star polycation (SPc)-based delivery platform with strong protection and efficient delivery to develop a self-assembled RNA pesticide with dual RNA interference (RNAi) targets. Results The nanocarrier SPc was applied to assemble with dsRNA via electrostatic interaction, hydrogen bond and Van der Waals force, and the self-complexation with SPc formed nanoscale dsRNA/SPc complex. The SPc could protect the dsRNA from the degradation by midgut fluid or RNase A, thus significantly increasing the stability of dsRNA under various environmental conditions. Meanwhile, the SPc was able to improve the translocation of dsRNA across insect cuticle, and increase its plant uptake. Then, dsECR-A and dsTre-1 fragments were individually screened, and the dsECR-A and dsTre-1 fragments with good control effects were co-expressed in pET28-BL21 (DE3) RNase III − system to prepare the dsECR-A + Tre-1/SPc complex. Both topical application and spraying of dsECR-A + Tre-1/SPc complex could effectively control a piercing-sucking agricultural pest Apolygus lucorum. The SPc-loaded dsECR-A + Tre-1 could up-regulate endocytosis-related genes and down-regulate cuticle biosynthesis-related genes, which primarily inhibited insect growth and development. Conclusions Our study comprehensively demonstrated the advantages of SPc-based dsRNA delivery platform, and developed a self-assembled RNA pesticide with dual RNAi targets, which provided a reference for the design of novel RNA pesticides. Graphical abstract |
| format | Article |
| id | doaj-art-b96b8f611b844cb88fcbf2e93a8c9042 |
| institution | Kabale University |
| issn | 1477-3155 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-b96b8f611b844cb88fcbf2e93a8c90422025-02-09T12:53:08ZengBMCJournal of Nanobiotechnology1477-31552025-02-0123111710.1186/s12951-025-03155-xNano-delivery platform with strong protection and efficient delivery: preparation of self-assembled RNA pesticide with dual RNAi targets against Apolygus lucorumHeng Qiao0Qinhong Jiang1Jing Zhao2Liubin Xiao3Keyan Zhu-Salzman4Dejin Xu5Guangchun Xu6Jie Shen7Aiguo Gu8Dejun Hao9Shuo Yan10Yongan Tan11Institute of Plant Protection, Jiangsu Academy of Agricultural SciencesDepartment of Plant Biosecurity and MOA Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural UniversityInstitute of Plant Protection, Jiangsu Academy of Agricultural SciencesInstitute of Plant Protection, Jiangsu Academy of Agricultural SciencesDepartment of Entomology, Texas A&M UniversityInstitute of Plant Protection, Jiangsu Academy of Agricultural SciencesInstitute of Plant Protection, Jiangsu Academy of Agricultural SciencesDepartment of Plant Biosecurity and MOA Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural UniversityJiangsu Product Quality Testing & Inspection InstituteCollege of Forestry, Nanjing Forestry UniversityDepartment of Plant Biosecurity and MOA Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural UniversityInstitute of Plant Protection, Jiangsu Academy of Agricultural SciencesAbstract Background RNA pesticide is regarded as the “third revolution in the history of pesticides”. However, the double-stranded RNA (dsRNA) is easily degraded in the environment, and its delivery efficiency is not sufficient for pest management. This study aimed to construct a star polycation (SPc)-based delivery platform with strong protection and efficient delivery to develop a self-assembled RNA pesticide with dual RNA interference (RNAi) targets. Results The nanocarrier SPc was applied to assemble with dsRNA via electrostatic interaction, hydrogen bond and Van der Waals force, and the self-complexation with SPc formed nanoscale dsRNA/SPc complex. The SPc could protect the dsRNA from the degradation by midgut fluid or RNase A, thus significantly increasing the stability of dsRNA under various environmental conditions. Meanwhile, the SPc was able to improve the translocation of dsRNA across insect cuticle, and increase its plant uptake. Then, dsECR-A and dsTre-1 fragments were individually screened, and the dsECR-A and dsTre-1 fragments with good control effects were co-expressed in pET28-BL21 (DE3) RNase III − system to prepare the dsECR-A + Tre-1/SPc complex. Both topical application and spraying of dsECR-A + Tre-1/SPc complex could effectively control a piercing-sucking agricultural pest Apolygus lucorum. The SPc-loaded dsECR-A + Tre-1 could up-regulate endocytosis-related genes and down-regulate cuticle biosynthesis-related genes, which primarily inhibited insect growth and development. Conclusions Our study comprehensively demonstrated the advantages of SPc-based dsRNA delivery platform, and developed a self-assembled RNA pesticide with dual RNAi targets, which provided a reference for the design of novel RNA pesticides. Graphical abstracthttps://doi.org/10.1186/s12951-025-03155-xApolygus lucorumDelivery systemNanocarrierRNA interferenceRNA pesticide |
| spellingShingle | Heng Qiao Qinhong Jiang Jing Zhao Liubin Xiao Keyan Zhu-Salzman Dejin Xu Guangchun Xu Jie Shen Aiguo Gu Dejun Hao Shuo Yan Yongan Tan Nano-delivery platform with strong protection and efficient delivery: preparation of self-assembled RNA pesticide with dual RNAi targets against Apolygus lucorum Journal of Nanobiotechnology Apolygus lucorum Delivery system Nanocarrier RNA interference RNA pesticide |
| title | Nano-delivery platform with strong protection and efficient delivery: preparation of self-assembled RNA pesticide with dual RNAi targets against Apolygus lucorum |
| title_full | Nano-delivery platform with strong protection and efficient delivery: preparation of self-assembled RNA pesticide with dual RNAi targets against Apolygus lucorum |
| title_fullStr | Nano-delivery platform with strong protection and efficient delivery: preparation of self-assembled RNA pesticide with dual RNAi targets against Apolygus lucorum |
| title_full_unstemmed | Nano-delivery platform with strong protection and efficient delivery: preparation of self-assembled RNA pesticide with dual RNAi targets against Apolygus lucorum |
| title_short | Nano-delivery platform with strong protection and efficient delivery: preparation of self-assembled RNA pesticide with dual RNAi targets against Apolygus lucorum |
| title_sort | nano delivery platform with strong protection and efficient delivery preparation of self assembled rna pesticide with dual rnai targets against apolygus lucorum |
| topic | Apolygus lucorum Delivery system Nanocarrier RNA interference RNA pesticide |
| url | https://doi.org/10.1186/s12951-025-03155-x |
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