Iron nanoparticle/carbon nanotube composite as oxidase-like nanozyme for visual analysis of total antioxidant capacity
Total antioxidant capacity (TAC) is an important indicator for assessing the merit of natural plants and foods. Herein, a visual TAC assay is developed based on the oxidase-like activity of nitrogen-doped carbon nanotubes loaded with Fe nanoparticles (FeNPs@NCNT), which is prepared via high-temperat...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157524009817 |
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| author | Junlin Liu Sophia Xie Nan Wang Zhongyue Sun Lina Tang Guo-jun Zhang John Tressel Yulin Zhang Yujie Sun Shaowei Chen |
| author_facet | Junlin Liu Sophia Xie Nan Wang Zhongyue Sun Lina Tang Guo-jun Zhang John Tressel Yulin Zhang Yujie Sun Shaowei Chen |
| author_sort | Junlin Liu |
| collection | DOAJ |
| description | Total antioxidant capacity (TAC) is an important indicator for assessing the merit of natural plants and foods. Herein, a visual TAC assay is developed based on the oxidase-like activity of nitrogen-doped carbon nanotubes loaded with Fe nanoparticles (FeNPs@NCNT), which is prepared via high-temperature pyrolysis of metal-organic framework precursors and can catalyze the oxidation of colorless o-phenylenediamine (OPD) to colored 2,3-diaminophenazine (DAP). The addition of antioxidants (e.g., quercetin) impedes the formation of DAP, diminishing the color change, which can be analyzed via the RGB values obtained with a smartphone color-recognition APP, “Color Picker”. The change of the optical signal can also be analyzed in the fluorescence mode. These two detection modes yield consistent TAC analysis of actual plant samples, in accord with results from the standard ABTS method. Results from this study highlight the unique potential of nanozymes in the development of effective TAC analysis platforms for natural plants and food. |
| format | Article |
| id | doaj-art-bc4e20d7447f4f3392becc2a24f32ae0 |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-bc4e20d7447f4f3392becc2a24f32ae02025-02-12T05:31:56ZengElsevierFood Chemistry: X2590-15752025-01-0125102093Iron nanoparticle/carbon nanotube composite as oxidase-like nanozyme for visual analysis of total antioxidant capacityJunlin Liu0Sophia Xie1Nan Wang2Zhongyue Sun3Lina Tang4Guo-jun Zhang5John Tressel6Yulin Zhang7Yujie Sun8Shaowei Chen9School of Laboratory Medicine, Hubei University of Chinese Medicine, Hubei Shizhen Laboratory, Wuhan 430065, ChinaWuhan Britain-China School, Wuhan 430033, ChinaDepartment of Physics, Jinan University, Guangzhou 510632, ChinaSchool of Laboratory Medicine, Hubei University of Chinese Medicine, Hubei Shizhen Laboratory, Wuhan 430065, ChinaSchool of Laboratory Medicine, Hubei University of Chinese Medicine, Hubei Shizhen Laboratory, Wuhan 430065, ChinaSchool of Laboratory Medicine, Hubei University of Chinese Medicine, Hubei Shizhen Laboratory, Wuhan 430065, ChinaDepartment of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95060, USASchool of Laboratory Medicine, Hubei University of Chinese Medicine, Hubei Shizhen Laboratory, Wuhan 430065, China; Hubei Key Laboratory of Resources and Chemistry of Chinese Medicine, School of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China; Corresponding authors.School of Laboratory Medicine, Hubei University of Chinese Medicine, Hubei Shizhen Laboratory, Wuhan 430065, China; Corresponding authors.Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95060, USA; Corresponding authors.Total antioxidant capacity (TAC) is an important indicator for assessing the merit of natural plants and foods. Herein, a visual TAC assay is developed based on the oxidase-like activity of nitrogen-doped carbon nanotubes loaded with Fe nanoparticles (FeNPs@NCNT), which is prepared via high-temperature pyrolysis of metal-organic framework precursors and can catalyze the oxidation of colorless o-phenylenediamine (OPD) to colored 2,3-diaminophenazine (DAP). The addition of antioxidants (e.g., quercetin) impedes the formation of DAP, diminishing the color change, which can be analyzed via the RGB values obtained with a smartphone color-recognition APP, “Color Picker”. The change of the optical signal can also be analyzed in the fluorescence mode. These two detection modes yield consistent TAC analysis of actual plant samples, in accord with results from the standard ABTS method. Results from this study highlight the unique potential of nanozymes in the development of effective TAC analysis platforms for natural plants and food.http://www.sciencedirect.com/science/article/pii/S2590157524009817Carbon nanotubeIron nanoparticleOxidaseNanozymeTotal antioxidant capacityFluorescence |
| spellingShingle | Junlin Liu Sophia Xie Nan Wang Zhongyue Sun Lina Tang Guo-jun Zhang John Tressel Yulin Zhang Yujie Sun Shaowei Chen Iron nanoparticle/carbon nanotube composite as oxidase-like nanozyme for visual analysis of total antioxidant capacity Food Chemistry: X Carbon nanotube Iron nanoparticle Oxidase Nanozyme Total antioxidant capacity Fluorescence |
| title | Iron nanoparticle/carbon nanotube composite as oxidase-like nanozyme for visual analysis of total antioxidant capacity |
| title_full | Iron nanoparticle/carbon nanotube composite as oxidase-like nanozyme for visual analysis of total antioxidant capacity |
| title_fullStr | Iron nanoparticle/carbon nanotube composite as oxidase-like nanozyme for visual analysis of total antioxidant capacity |
| title_full_unstemmed | Iron nanoparticle/carbon nanotube composite as oxidase-like nanozyme for visual analysis of total antioxidant capacity |
| title_short | Iron nanoparticle/carbon nanotube composite as oxidase-like nanozyme for visual analysis of total antioxidant capacity |
| title_sort | iron nanoparticle carbon nanotube composite as oxidase like nanozyme for visual analysis of total antioxidant capacity |
| topic | Carbon nanotube Iron nanoparticle Oxidase Nanozyme Total antioxidant capacity Fluorescence |
| url | http://www.sciencedirect.com/science/article/pii/S2590157524009817 |
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