Biochemical and anatomical characteristics associated with browning of Bambusa lako Widjaja (Poaceae) in vitro shoot explants
Browning is a limiting factor for the successful propagation of several, mainly woody, plant species through tissue culture. Bamboos are not exempt from this constraint, which has been described as particularly critical for the micropropagation of several Bambusa species, such as B. lako. To underst...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Advances in Bamboo Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773139125000084 |
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| author | Andrea Holst Paula Carvajal-Campos Yanely Marisol Canales-Ochoa Andrea Irías-Mata Laura Vega-Fernández Miguel Benavides-Acevedo Paul Solórzano-Cascante Víctor M. Jiménez |
| author_facet | Andrea Holst Paula Carvajal-Campos Yanely Marisol Canales-Ochoa Andrea Irías-Mata Laura Vega-Fernández Miguel Benavides-Acevedo Paul Solórzano-Cascante Víctor M. Jiménez |
| author_sort | Andrea Holst |
| collection | DOAJ |
| description | Browning is a limiting factor for the successful propagation of several, mainly woody, plant species through tissue culture. Bamboos are not exempt from this constraint, which has been described as particularly critical for the micropropagation of several Bambusa species, such as B. lako. To understand the browning process in this species, we investigated whether the browning of in vitro nodal explants was linked to the accumulation of phenolic compounds, polyphenol oxidase activity, and oxidative stress as indicated by the presence of malondialdehyde. A four-point Browning Gradient Index was developed to evaluate these biochemical parameters. We observed an increase in the concentration of total phenolics, polyphenol oxidase activity, and malondialdehyde levels between green and fully brown grades. Histological analysis revealed a greater accumulation of protein in the green shoots compared to brown tissues. Polyphenols and peroxidase were localized in the vascular bundles in all browning grades. |
| format | Article |
| id | doaj-art-bc820570e4ab427e8ad2b2432ffdef50 |
| institution | Kabale University |
| issn | 2773-1391 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Advances in Bamboo Science |
| spelling | doaj-art-bc820570e4ab427e8ad2b2432ffdef502025-02-07T04:48:35ZengElsevierAdvances in Bamboo Science2773-13912025-02-0110100129Biochemical and anatomical characteristics associated with browning of Bambusa lako Widjaja (Poaceae) in vitro shoot explantsAndrea Holst0Paula Carvajal-Campos1Yanely Marisol Canales-Ochoa2Andrea Irías-Mata3Laura Vega-Fernández4Miguel Benavides-Acevedo5Paul Solórzano-Cascante6Víctor M. Jiménez7CIGRAS, Universidad de Costa Rica, San Pedro 11501-2060, Costa RicaCIGRAS, Universidad de Costa Rica, San Pedro 11501-2060, Costa Rica; IIA, Universidad de Costa Rica, San Pedro 11501-2060, Costa RicaCIGRAS, Universidad de Costa Rica, San Pedro 11501-2060, Costa RicaCIGRAS, Universidad de Costa Rica, San Pedro 11501-2060, Costa RicaCIGRAS, Universidad de Costa Rica, San Pedro 11501-2060, Costa RicaCIGRAS, Universidad de Costa Rica, San Pedro 11501-2060, Costa RicaLeibniz Institute of Plant Genetics and Crop Plant Research (IPK), Seeland, GermanyCIGRAS, Universidad de Costa Rica, San Pedro 11501-2060, Costa Rica; IIA, Universidad de Costa Rica, San Pedro 11501-2060, Costa Rica; Corresponding author at: CIGRAS, Universidad de Costa Rica, San Pedro 11501-2060, Costa Rica.Browning is a limiting factor for the successful propagation of several, mainly woody, plant species through tissue culture. Bamboos are not exempt from this constraint, which has been described as particularly critical for the micropropagation of several Bambusa species, such as B. lako. To understand the browning process in this species, we investigated whether the browning of in vitro nodal explants was linked to the accumulation of phenolic compounds, polyphenol oxidase activity, and oxidative stress as indicated by the presence of malondialdehyde. A four-point Browning Gradient Index was developed to evaluate these biochemical parameters. We observed an increase in the concentration of total phenolics, polyphenol oxidase activity, and malondialdehyde levels between green and fully brown grades. Histological analysis revealed a greater accumulation of protein in the green shoots compared to brown tissues. Polyphenols and peroxidase were localized in the vascular bundles in all browning grades.http://www.sciencedirect.com/science/article/pii/S2773139125000084Indirect enzymatic histochemistryMalondialdehydeOxidationPhenolic compoundsPolyphenol oxidase |
| spellingShingle | Andrea Holst Paula Carvajal-Campos Yanely Marisol Canales-Ochoa Andrea Irías-Mata Laura Vega-Fernández Miguel Benavides-Acevedo Paul Solórzano-Cascante Víctor M. Jiménez Biochemical and anatomical characteristics associated with browning of Bambusa lako Widjaja (Poaceae) in vitro shoot explants Advances in Bamboo Science Indirect enzymatic histochemistry Malondialdehyde Oxidation Phenolic compounds Polyphenol oxidase |
| title | Biochemical and anatomical characteristics associated with browning of Bambusa lako Widjaja (Poaceae) in vitro shoot explants |
| title_full | Biochemical and anatomical characteristics associated with browning of Bambusa lako Widjaja (Poaceae) in vitro shoot explants |
| title_fullStr | Biochemical and anatomical characteristics associated with browning of Bambusa lako Widjaja (Poaceae) in vitro shoot explants |
| title_full_unstemmed | Biochemical and anatomical characteristics associated with browning of Bambusa lako Widjaja (Poaceae) in vitro shoot explants |
| title_short | Biochemical and anatomical characteristics associated with browning of Bambusa lako Widjaja (Poaceae) in vitro shoot explants |
| title_sort | biochemical and anatomical characteristics associated with browning of bambusa lako widjaja poaceae in vitro shoot explants |
| topic | Indirect enzymatic histochemistry Malondialdehyde Oxidation Phenolic compounds Polyphenol oxidase |
| url | http://www.sciencedirect.com/science/article/pii/S2773139125000084 |
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