Synthesis and Characterisation of Gold Nanoparticles from Acacia Leaf and to Evaluate Anticariogenic Activity
Objective: To synthesise the gold nanoparticles (AuNPs) using Acacia catechu through biogenic synthesis and evaluate their antimicrobial efficacy against S. mutans and E. coli in vitro. Methods: Green synthesised AuNPs were characterised using the ultraviolet–visible (UV-Vis) spectroscopy, and the s...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2023-09-01
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| Series: | Indian Journal of Dental Research |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/ijdr.ijdr_987_22 |
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| Summary: | Objective:
To synthesise the gold nanoparticles (AuNPs) using Acacia catechu through biogenic synthesis and evaluate their antimicrobial efficacy against S. mutans and E. coli in vitro.
Methods:
Green synthesised AuNPs were characterised using the ultraviolet–visible (UV-Vis) spectroscopy, and the size and shape of the synthesised nanoparticles were evaluated using the transmission electron microscopy (TEM). The antimicrobial efficacy of AuNPs (30/60/100 μl) against S. mutans/E. coli was evaluated on the Mueller–Hinton agar by measuring the zone of inhibition (ZOI) with ampicillin (15 μl) as a positive control.
Results:
The synthesised AuNPs were confirmed using the UV-Vis spectroscopy with peaks at 540 nm, and the size of the particle estimated using the TEM was between 5 and 15 nm. The antimicrobial efficacy of AuNPs was comparable to that of ampicillin against S. mutans/E. coli, but the difference was not significant. The antimicrobial effects increased in a dose-dependent fashion but were comparable across all concentrations and ampicillin.
Conclusion:
Green synthesised AuNPs exhibited significant antibacterial activity against S. mutans and E. coli at par with commercial ampicillin and demonstrated the potential towards anticariogenic agent for future use in dentistry. |
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| ISSN: | 0970-9290 1998-3603 |