Synthesis and Characterisation of Gold Nanoparticles from Acacia Leaf and to Evaluate Anticariogenic Activity
Objective: To synthesise the gold nanoparticles (AuNPs) using Acacia catechu through biogenic synthesis and evaluate their antimicrobial efficacy against S. mutans and E. coli in vitro. Methods: Green synthesised AuNPs were characterised using the ultraviolet–visible (UV-Vis) spectroscopy, and the s...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-09-01
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| Series: | Indian Journal of Dental Research |
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| Online Access: | https://journals.lww.com/10.4103/ijdr.ijdr_987_22 |
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| author | Maher AbdelFattah Al Shayeb Naresh Yedthare Shetty Anas Al Jadaa Syed Kuduruthullah |
| author_facet | Maher AbdelFattah Al Shayeb Naresh Yedthare Shetty Anas Al Jadaa Syed Kuduruthullah |
| author_sort | Maher AbdelFattah Al Shayeb |
| collection | DOAJ |
| description | Objective:
To synthesise the gold nanoparticles (AuNPs) using Acacia catechu through biogenic synthesis and evaluate their antimicrobial efficacy against S. mutans and E. coli in vitro.
Methods:
Green synthesised AuNPs were characterised using the ultraviolet–visible (UV-Vis) spectroscopy, and the size and shape of the synthesised nanoparticles were evaluated using the transmission electron microscopy (TEM). The antimicrobial efficacy of AuNPs (30/60/100 μl) against S. mutans/E. coli was evaluated on the Mueller–Hinton agar by measuring the zone of inhibition (ZOI) with ampicillin (15 μl) as a positive control.
Results:
The synthesised AuNPs were confirmed using the UV-Vis spectroscopy with peaks at 540 nm, and the size of the particle estimated using the TEM was between 5 and 15 nm. The antimicrobial efficacy of AuNPs was comparable to that of ampicillin against S. mutans/E. coli, but the difference was not significant. The antimicrobial effects increased in a dose-dependent fashion but were comparable across all concentrations and ampicillin.
Conclusion:
Green synthesised AuNPs exhibited significant antibacterial activity against S. mutans and E. coli at par with commercial ampicillin and demonstrated the potential towards anticariogenic agent for future use in dentistry. |
| format | Article |
| id | doaj-art-bddaa00aa5994903a2208afc91478b4a |
| institution | Kabale University |
| issn | 0970-9290 1998-3603 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Dental Research |
| spelling | doaj-art-bddaa00aa5994903a2208afc91478b4a2025-02-09T09:41:27ZengWolters Kluwer Medknow PublicationsIndian Journal of Dental Research0970-92901998-36032023-09-0134219619810.4103/ijdr.ijdr_987_22Synthesis and Characterisation of Gold Nanoparticles from Acacia Leaf and to Evaluate Anticariogenic ActivityMaher AbdelFattah Al ShayebNaresh Yedthare ShettyAnas Al JadaaSyed KuduruthullahObjective: To synthesise the gold nanoparticles (AuNPs) using Acacia catechu through biogenic synthesis and evaluate their antimicrobial efficacy against S. mutans and E. coli in vitro. Methods: Green synthesised AuNPs were characterised using the ultraviolet–visible (UV-Vis) spectroscopy, and the size and shape of the synthesised nanoparticles were evaluated using the transmission electron microscopy (TEM). The antimicrobial efficacy of AuNPs (30/60/100 μl) against S. mutans/E. coli was evaluated on the Mueller–Hinton agar by measuring the zone of inhibition (ZOI) with ampicillin (15 μl) as a positive control. Results: The synthesised AuNPs were confirmed using the UV-Vis spectroscopy with peaks at 540 nm, and the size of the particle estimated using the TEM was between 5 and 15 nm. The antimicrobial efficacy of AuNPs was comparable to that of ampicillin against S. mutans/E. coli, but the difference was not significant. The antimicrobial effects increased in a dose-dependent fashion but were comparable across all concentrations and ampicillin. Conclusion: Green synthesised AuNPs exhibited significant antibacterial activity against S. mutans and E. coli at par with commercial ampicillin and demonstrated the potential towards anticariogenic agent for future use in dentistry.https://journals.lww.com/10.4103/ijdr.ijdr_987_22cariesgold nanoparticlesgreen synthesiss. mutans |
| spellingShingle | Maher AbdelFattah Al Shayeb Naresh Yedthare Shetty Anas Al Jadaa Syed Kuduruthullah Synthesis and Characterisation of Gold Nanoparticles from Acacia Leaf and to Evaluate Anticariogenic Activity Indian Journal of Dental Research caries gold nanoparticles green synthesis s. mutans |
| title | Synthesis and Characterisation of Gold Nanoparticles from Acacia Leaf and to Evaluate Anticariogenic Activity |
| title_full | Synthesis and Characterisation of Gold Nanoparticles from Acacia Leaf and to Evaluate Anticariogenic Activity |
| title_fullStr | Synthesis and Characterisation of Gold Nanoparticles from Acacia Leaf and to Evaluate Anticariogenic Activity |
| title_full_unstemmed | Synthesis and Characterisation of Gold Nanoparticles from Acacia Leaf and to Evaluate Anticariogenic Activity |
| title_short | Synthesis and Characterisation of Gold Nanoparticles from Acacia Leaf and to Evaluate Anticariogenic Activity |
| title_sort | synthesis and characterisation of gold nanoparticles from acacia leaf and to evaluate anticariogenic activity |
| topic | caries gold nanoparticles green synthesis s. mutans |
| url | https://journals.lww.com/10.4103/ijdr.ijdr_987_22 |
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