Coupling of an Au@AgPt nanozyme array with an micrococcal nuclease-specific responsiveness strategy for colorimetric/SERS sensing of Staphylococcus aureus in patients with sepsis
Rapid and ultrasensitive detection of pathogen-associated biomarkers is vital for the early diagnosis and therapy of bacterial infections. Herein, we developed a close-packed and ordered Au@AgPt array coupled with a cascade triggering strategy for surface-enhanced Raman scattering (SERS) and colorim...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-02-01
|
| Series: | Journal of Pharmaceutical Analysis |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095177924001825 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823864430771830784 |
|---|---|
| author | Xueqin Huang Yingqi Yang Hanlin Zhou Liping Hu Annan Yang Hua Jin Biying Zheng Jiang Pi Jun Xu Pinghua Sun Huai-Hong Cai Xujing Liang Bin Pan Junxia Zheng Haibo Zhou |
| author_facet | Xueqin Huang Yingqi Yang Hanlin Zhou Liping Hu Annan Yang Hua Jin Biying Zheng Jiang Pi Jun Xu Pinghua Sun Huai-Hong Cai Xujing Liang Bin Pan Junxia Zheng Haibo Zhou |
| author_sort | Xueqin Huang |
| collection | DOAJ |
| description | Rapid and ultrasensitive detection of pathogen-associated biomarkers is vital for the early diagnosis and therapy of bacterial infections. Herein, we developed a close-packed and ordered Au@AgPt array coupled with a cascade triggering strategy for surface-enhanced Raman scattering (SERS) and colorimetric identification of the Staphylococcus aureus biomarker micrococcal nuclease (MNase) in serum samples. The trimetallic Au@AgPt nanozymes can catalyze the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB) molecules to SERS-enhanced oxidized TMB (oxTMB), accompanied by the color change from colorless to blue. In the presence of S. aureus, the secreted MNase preferentially cut the nucleobase AT-rich regions of DNA sequences on magnetic beads (MBs) to release alkaline phosphatase (ALP), which subsequently mediated the oxTMB reduction for inducing the colorimetric/SERS signal fade away. Using this “on-to-off” triggering strategy, the target S. aureus can be recorded in a wide linear range with a limit of detection of 38 CFU/mL in the colorimetric mode and 6 CFU/mL in the SERS mode. Meanwhile, the MNase-mediated strategy characterized by high specificity and sensitivity successfully discriminated between patients with sepsis (n = 7) and healthy participants (n = 3), as well as monitored the prognostic progression of the disease (n = 2). Overall, benefiting from highly active and dense “hot spot” substrate, MNase-mediated cascade response strategy, and colorimetric/SERS dual-signal output, this methodology will offer a promising avenue for the early diagnosis of S. aureus infection. |
| format | Article |
| id | doaj-art-be5fb8d6ffd54d648785df65b7a06f3a |
| institution | Kabale University |
| issn | 2095-1779 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmaceutical Analysis |
| spelling | doaj-art-be5fb8d6ffd54d648785df65b7a06f3a2025-02-09T05:00:03ZengElsevierJournal of Pharmaceutical Analysis2095-17792025-02-01152101085Coupling of an Au@AgPt nanozyme array with an micrococcal nuclease-specific responsiveness strategy for colorimetric/SERS sensing of Staphylococcus aureus in patients with sepsisXueqin Huang0Yingqi Yang1Hanlin Zhou2Liping Hu3Annan Yang4Hua Jin5Biying Zheng6Jiang Pi7Jun Xu8Pinghua Sun9Huai-Hong Cai10Xujing Liang11Bin Pan12Junxia Zheng13Haibo Zhou14College of Pharmacy, The Second Clinical Medical College (Shenzhen People's Hospital), The Fifth Affiliated Hospital, Jinan University, Guangzhou, 510632, China; Research Center of Nano Technology and Application Engineering, The First Dongguan Affiliated Hospital, School of Medical Technology, Guangdong Medical University, Dongguan, Guangdong, 523000, ChinaCollege of Pharmacy, The Second Clinical Medical College (Shenzhen People's Hospital), The Fifth Affiliated Hospital, Jinan University, Guangzhou, 510632, ChinaCollege of Pharmacy, The Second Clinical Medical College (Shenzhen People's Hospital), The Fifth Affiliated Hospital, Jinan University, Guangzhou, 510632, ChinaCollege of Pharmacy, The Second Clinical Medical College (Shenzhen People's Hospital), The Fifth Affiliated Hospital, Jinan University, Guangzhou, 510632, ChinaCollege of Pharmacy, The Second Clinical Medical College (Shenzhen People's Hospital), The Fifth Affiliated Hospital, Jinan University, Guangzhou, 510632, ChinaResearch Center of Nano Technology and Application Engineering, The First Dongguan Affiliated Hospital, School of Medical Technology, Guangdong Medical University, Dongguan, Guangdong, 523000, ChinaResearch Center of Nano Technology and Application Engineering, The First Dongguan Affiliated Hospital, School of Medical Technology, Guangdong Medical University, Dongguan, Guangdong, 523000, ChinaResearch Center of Nano Technology and Application Engineering, The First Dongguan Affiliated Hospital, School of Medical Technology, Guangdong Medical University, Dongguan, Guangdong, 523000, ChinaCollege of Pharmacy, The Second Clinical Medical College (Shenzhen People's Hospital), The Fifth Affiliated Hospital, Jinan University, Guangzhou, 510632, ChinaCollege of Pharmacy, The Second Clinical Medical College (Shenzhen People's Hospital), The Fifth Affiliated Hospital, Jinan University, Guangzhou, 510632, China; School of Pharmacy, Shihezi University, Shihezi, Xinjiang, 832003, ChinaCollege of Chemistry and Materials Science, Jinan University, Guangzhou, 510632, ChinaThe First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, 510632, China; Corresponding author.The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, 510632, China; Corresponding author.School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, 510006, China; Corresponding author.College of Pharmacy, The Second Clinical Medical College (Shenzhen People's Hospital), The Fifth Affiliated Hospital, Jinan University, Guangzhou, 510632, China; Corresponding author.Rapid and ultrasensitive detection of pathogen-associated biomarkers is vital for the early diagnosis and therapy of bacterial infections. Herein, we developed a close-packed and ordered Au@AgPt array coupled with a cascade triggering strategy for surface-enhanced Raman scattering (SERS) and colorimetric identification of the Staphylococcus aureus biomarker micrococcal nuclease (MNase) in serum samples. The trimetallic Au@AgPt nanozymes can catalyze the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB) molecules to SERS-enhanced oxidized TMB (oxTMB), accompanied by the color change from colorless to blue. In the presence of S. aureus, the secreted MNase preferentially cut the nucleobase AT-rich regions of DNA sequences on magnetic beads (MBs) to release alkaline phosphatase (ALP), which subsequently mediated the oxTMB reduction for inducing the colorimetric/SERS signal fade away. Using this “on-to-off” triggering strategy, the target S. aureus can be recorded in a wide linear range with a limit of detection of 38 CFU/mL in the colorimetric mode and 6 CFU/mL in the SERS mode. Meanwhile, the MNase-mediated strategy characterized by high specificity and sensitivity successfully discriminated between patients with sepsis (n = 7) and healthy participants (n = 3), as well as monitored the prognostic progression of the disease (n = 2). Overall, benefiting from highly active and dense “hot spot” substrate, MNase-mediated cascade response strategy, and colorimetric/SERS dual-signal output, this methodology will offer a promising avenue for the early diagnosis of S. aureus infection.http://www.sciencedirect.com/science/article/pii/S2095177924001825Au@AgPt nanoarraysSurface-enhanced Raman scatteringColorimetryCascade response strategyMNase |
| spellingShingle | Xueqin Huang Yingqi Yang Hanlin Zhou Liping Hu Annan Yang Hua Jin Biying Zheng Jiang Pi Jun Xu Pinghua Sun Huai-Hong Cai Xujing Liang Bin Pan Junxia Zheng Haibo Zhou Coupling of an Au@AgPt nanozyme array with an micrococcal nuclease-specific responsiveness strategy for colorimetric/SERS sensing of Staphylococcus aureus in patients with sepsis Journal of Pharmaceutical Analysis Au@AgPt nanoarrays Surface-enhanced Raman scattering Colorimetry Cascade response strategy MNase |
| title | Coupling of an Au@AgPt nanozyme array with an micrococcal nuclease-specific responsiveness strategy for colorimetric/SERS sensing of Staphylococcus aureus in patients with sepsis |
| title_full | Coupling of an Au@AgPt nanozyme array with an micrococcal nuclease-specific responsiveness strategy for colorimetric/SERS sensing of Staphylococcus aureus in patients with sepsis |
| title_fullStr | Coupling of an Au@AgPt nanozyme array with an micrococcal nuclease-specific responsiveness strategy for colorimetric/SERS sensing of Staphylococcus aureus in patients with sepsis |
| title_full_unstemmed | Coupling of an Au@AgPt nanozyme array with an micrococcal nuclease-specific responsiveness strategy for colorimetric/SERS sensing of Staphylococcus aureus in patients with sepsis |
| title_short | Coupling of an Au@AgPt nanozyme array with an micrococcal nuclease-specific responsiveness strategy for colorimetric/SERS sensing of Staphylococcus aureus in patients with sepsis |
| title_sort | coupling of an au agpt nanozyme array with an micrococcal nuclease specific responsiveness strategy for colorimetric sers sensing of staphylococcus aureus in patients with sepsis |
| topic | Au@AgPt nanoarrays Surface-enhanced Raman scattering Colorimetry Cascade response strategy MNase |
| url | http://www.sciencedirect.com/science/article/pii/S2095177924001825 |
| work_keys_str_mv | AT xueqinhuang couplingofanauagptnanozymearraywithanmicrococcalnucleasespecificresponsivenessstrategyforcolorimetricserssensingofstaphylococcusaureusinpatientswithsepsis AT yingqiyang couplingofanauagptnanozymearraywithanmicrococcalnucleasespecificresponsivenessstrategyforcolorimetricserssensingofstaphylococcusaureusinpatientswithsepsis AT hanlinzhou couplingofanauagptnanozymearraywithanmicrococcalnucleasespecificresponsivenessstrategyforcolorimetricserssensingofstaphylococcusaureusinpatientswithsepsis AT lipinghu couplingofanauagptnanozymearraywithanmicrococcalnucleasespecificresponsivenessstrategyforcolorimetricserssensingofstaphylococcusaureusinpatientswithsepsis AT annanyang couplingofanauagptnanozymearraywithanmicrococcalnucleasespecificresponsivenessstrategyforcolorimetricserssensingofstaphylococcusaureusinpatientswithsepsis AT huajin couplingofanauagptnanozymearraywithanmicrococcalnucleasespecificresponsivenessstrategyforcolorimetricserssensingofstaphylococcusaureusinpatientswithsepsis AT biyingzheng couplingofanauagptnanozymearraywithanmicrococcalnucleasespecificresponsivenessstrategyforcolorimetricserssensingofstaphylococcusaureusinpatientswithsepsis AT jiangpi couplingofanauagptnanozymearraywithanmicrococcalnucleasespecificresponsivenessstrategyforcolorimetricserssensingofstaphylococcusaureusinpatientswithsepsis AT junxu couplingofanauagptnanozymearraywithanmicrococcalnucleasespecificresponsivenessstrategyforcolorimetricserssensingofstaphylococcusaureusinpatientswithsepsis AT pinghuasun couplingofanauagptnanozymearraywithanmicrococcalnucleasespecificresponsivenessstrategyforcolorimetricserssensingofstaphylococcusaureusinpatientswithsepsis AT huaihongcai couplingofanauagptnanozymearraywithanmicrococcalnucleasespecificresponsivenessstrategyforcolorimetricserssensingofstaphylococcusaureusinpatientswithsepsis AT xujingliang couplingofanauagptnanozymearraywithanmicrococcalnucleasespecificresponsivenessstrategyforcolorimetricserssensingofstaphylococcusaureusinpatientswithsepsis AT binpan couplingofanauagptnanozymearraywithanmicrococcalnucleasespecificresponsivenessstrategyforcolorimetricserssensingofstaphylococcusaureusinpatientswithsepsis AT junxiazheng couplingofanauagptnanozymearraywithanmicrococcalnucleasespecificresponsivenessstrategyforcolorimetricserssensingofstaphylococcusaureusinpatientswithsepsis AT haibozhou couplingofanauagptnanozymearraywithanmicrococcalnucleasespecificresponsivenessstrategyforcolorimetricserssensingofstaphylococcusaureusinpatientswithsepsis |