OPTIMIZATION OF NEUROTRANSMITTERS SEPARATION UNDER HILIC CONDITIONS
This paper presents the optimization of a hydrophilic interaction chromatographic method for the separation of eleven catecholamines, indolamines and their precursors and metabolites. The studied parameters are: the organic modifier nature and percentage, the salt nature and concentration, the mobi...
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| Format: | Article |
| Language: | English |
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Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
2017-03-01
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| Series: | Journal of Engineering Studies and Research |
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| Online Access: | https://jesr.ub.ro/index.php/1/article/view/90 |
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| _version_ | 1823863631164473344 |
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| author | RALUCA TAMPU CATALIN TAMPU |
| author_facet | RALUCA TAMPU CATALIN TAMPU |
| author_sort | RALUCA TAMPU |
| collection | DOAJ |
| description |
This paper presents the optimization of a hydrophilic interaction chromatographic method for the separation of eleven catecholamines, indolamines and their precursors and metabolites. The studied parameters are: the organic modifier nature and percentage, the salt nature and concentration, the mobile phase pH and the column temperature. The best results in terms of separation were obtained using a mobile phase composed of acetonitrile and ammonium formate (150 mM pH 3) 85:15 v:v.
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| format | Article |
| id | doaj-art-c087444ffcb9440db39acb3c54d2e590 |
| institution | Kabale University |
| issn | 2068-7559 2344-4932 |
| language | English |
| publishDate | 2017-03-01 |
| publisher | Alma Mater Publishing House "Vasile Alecsandri" University of Bacau |
| record_format | Article |
| series | Journal of Engineering Studies and Research |
| spelling | doaj-art-c087444ffcb9440db39acb3c54d2e5902025-02-09T11:38:06ZengAlma Mater Publishing House "Vasile Alecsandri" University of BacauJournal of Engineering Studies and Research2068-75592344-49322017-03-01231OPTIMIZATION OF NEUROTRANSMITTERS SEPARATION UNDER HILIC CONDITIONSRALUCA TAMPU0CATALIN TAMPU1“Vasile Alecsandri” University of Bacau, Calea Marasesti 157, Bacau, 600115, Romania “Vasile Alecsandri” University of Bacau, Calea Marasesti 157, Bacau, 600115, Romania This paper presents the optimization of a hydrophilic interaction chromatographic method for the separation of eleven catecholamines, indolamines and their precursors and metabolites. The studied parameters are: the organic modifier nature and percentage, the salt nature and concentration, the mobile phase pH and the column temperature. The best results in terms of separation were obtained using a mobile phase composed of acetonitrile and ammonium formate (150 mM pH 3) 85:15 v:v. https://jesr.ub.ro/index.php/1/article/view/90catecholaminesHILICneurotransmitters |
| spellingShingle | RALUCA TAMPU CATALIN TAMPU OPTIMIZATION OF NEUROTRANSMITTERS SEPARATION UNDER HILIC CONDITIONS Journal of Engineering Studies and Research catecholamines HILIC neurotransmitters |
| title | OPTIMIZATION OF NEUROTRANSMITTERS SEPARATION UNDER HILIC CONDITIONS |
| title_full | OPTIMIZATION OF NEUROTRANSMITTERS SEPARATION UNDER HILIC CONDITIONS |
| title_fullStr | OPTIMIZATION OF NEUROTRANSMITTERS SEPARATION UNDER HILIC CONDITIONS |
| title_full_unstemmed | OPTIMIZATION OF NEUROTRANSMITTERS SEPARATION UNDER HILIC CONDITIONS |
| title_short | OPTIMIZATION OF NEUROTRANSMITTERS SEPARATION UNDER HILIC CONDITIONS |
| title_sort | optimization of neurotransmitters separation under hilic conditions |
| topic | catecholamines HILIC neurotransmitters |
| url | https://jesr.ub.ro/index.php/1/article/view/90 |
| work_keys_str_mv | AT ralucatampu optimizationofneurotransmittersseparationunderhilicconditions AT catalintampu optimizationofneurotransmittersseparationunderhilicconditions |