Increased dicentric chromosome in peripheral lymphocytes is related to acute skin toxicity induced by radiotherapy in cancer patients
Objective: To investigate the impacts of radiotherapy (RT) on dicentric chromosomes in peripheral blood lymphocytes of cancer patients, in order to explore the relationship between dicentric chromosomes and RT-induced adverse reactions. Methods: A total of 33 cancer patients after postoperative RT i...
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Elsevier
2025-02-01
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| Series: | Radiation Medicine and Protection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666555724000728 |
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| author | Minjie Li Yu Gao Lin Han Ping Wang Jie Li Junhua Song Shasha Du Yunfei Zhang Yumin Lyu |
| author_facet | Minjie Li Yu Gao Lin Han Ping Wang Jie Li Junhua Song Shasha Du Yunfei Zhang Yumin Lyu |
| author_sort | Minjie Li |
| collection | DOAJ |
| description | Objective: To investigate the impacts of radiotherapy (RT) on dicentric chromosomes in peripheral blood lymphocytes of cancer patients, in order to explore the relationship between dicentric chromosomes and RT-induced adverse reactions. Methods: A total of 33 cancer patients after postoperative RT in a tertiary hospital from October 2021 to May 2022 were enrolled in this study. These patients were grouped according to the grade of acute skin and marrow toxicities determined based on the scoring criteria for acute morbidity developed by the Radiation Therapy Oncology Group (RTOG). Peripheral blood samples were collected from each patient before and after RT, followed by whole-blood lymphocyte culture and chromosome analysis. Dicentric chromosomes were searched automatically using a high-throughput chromosome analysis system and were then confirmed manually. Finally, the relationships of the frequency of dicentric chromosomes (also referred to as the dic frequency) with acute skin and marrow toxicities were assessed. Results: After RT, the mean counts of white blood cells (WBCs), lymphocytes, and platelets significantly decreased, while the red blood cells (RBCs) and hemoglobin notably increased (P < 0.001), while the dic frequency was elevated remarkably, significantly higher in patients with higher-grade (>1) acute skin toxicities compared to those with lower-grade (=1) acute skin toxicities (Z = −1.985, P = 0.047). However, no significant relationship was observed between the dic frequency and acute marrow toxicity after RT (P > 0.05). Logistic regression showed that radiosensitivity denoted by the post-RT dic frequency in peripheral blood lymphocytes produced insignificant impacts on the severity of RT-induced acute skin toxicities (P = 0.060). Conclusion: The elevated dic frequency in peripheral blood lymphocytes of cancer patients with higher-grade acute skin toxicities suggests enhanced radiosensitivity of peripheral blood lymphocytes, which is associated with the occurrence of RT-induced adverse reactions. |
| format | Article |
| id | doaj-art-c17bac57ee3942bea4dd3b0eac0ab611 |
| institution | Kabale University |
| issn | 2666-5557 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Radiation Medicine and Protection |
| spelling | doaj-art-c17bac57ee3942bea4dd3b0eac0ab6112025-02-12T05:32:53ZengElsevierRadiation Medicine and Protection2666-55572025-02-01612227Increased dicentric chromosome in peripheral lymphocytes is related to acute skin toxicity induced by radiotherapy in cancer patientsMinjie Li0Yu Gao1Lin Han2Ping Wang3Jie Li4Junhua Song5Shasha Du6Yunfei Zhang7Yumin Lyu8Department of Clinical Radiation Oncology, Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaHenan Key Laboratory of Medicine on Radiobiology and Epidemiology, Third People's Hospital of Henan Province (Henan Occupational Disease Hospital), Zhengzhou 450052, ChinaHenan Key Laboratory of Medicine on Radiobiology and Epidemiology, Third People's Hospital of Henan Province (Henan Occupational Disease Hospital), Zhengzhou 450052, ChinaHenan Key Laboratory of Medicine on Radiobiology and Epidemiology, Third People's Hospital of Henan Province (Henan Occupational Disease Hospital), Zhengzhou 450052, ChinaHenan Key Laboratory of Medicine on Radiobiology and Epidemiology, Third People's Hospital of Henan Province (Henan Occupational Disease Hospital), Zhengzhou 450052, ChinaChildren's Hospital of Zhejiang University School of Medicine, Hangzhou 310003, ChinaHenan Key Laboratory of Medicine on Radiobiology and Epidemiology, Third People's Hospital of Henan Province (Henan Occupational Disease Hospital), Zhengzhou 450052, ChinaHenan Key Laboratory of Medicine on Radiobiology and Epidemiology, Third People's Hospital of Henan Province (Henan Occupational Disease Hospital), Zhengzhou 450052, ChinaHenan Key Laboratory of Medicine on Radiobiology and Epidemiology, Third People's Hospital of Henan Province (Henan Occupational Disease Hospital), Zhengzhou 450052, China; Corresponding author.Objective: To investigate the impacts of radiotherapy (RT) on dicentric chromosomes in peripheral blood lymphocytes of cancer patients, in order to explore the relationship between dicentric chromosomes and RT-induced adverse reactions. Methods: A total of 33 cancer patients after postoperative RT in a tertiary hospital from October 2021 to May 2022 were enrolled in this study. These patients were grouped according to the grade of acute skin and marrow toxicities determined based on the scoring criteria for acute morbidity developed by the Radiation Therapy Oncology Group (RTOG). Peripheral blood samples were collected from each patient before and after RT, followed by whole-blood lymphocyte culture and chromosome analysis. Dicentric chromosomes were searched automatically using a high-throughput chromosome analysis system and were then confirmed manually. Finally, the relationships of the frequency of dicentric chromosomes (also referred to as the dic frequency) with acute skin and marrow toxicities were assessed. Results: After RT, the mean counts of white blood cells (WBCs), lymphocytes, and platelets significantly decreased, while the red blood cells (RBCs) and hemoglobin notably increased (P < 0.001), while the dic frequency was elevated remarkably, significantly higher in patients with higher-grade (>1) acute skin toxicities compared to those with lower-grade (=1) acute skin toxicities (Z = −1.985, P = 0.047). However, no significant relationship was observed between the dic frequency and acute marrow toxicity after RT (P > 0.05). Logistic regression showed that radiosensitivity denoted by the post-RT dic frequency in peripheral blood lymphocytes produced insignificant impacts on the severity of RT-induced acute skin toxicities (P = 0.060). Conclusion: The elevated dic frequency in peripheral blood lymphocytes of cancer patients with higher-grade acute skin toxicities suggests enhanced radiosensitivity of peripheral blood lymphocytes, which is associated with the occurrence of RT-induced adverse reactions.http://www.sciencedirect.com/science/article/pii/S2666555724000728RadiotherapyRadiation toxicityCytogeneticsDicentric chromosomeRadiosensitivity |
| spellingShingle | Minjie Li Yu Gao Lin Han Ping Wang Jie Li Junhua Song Shasha Du Yunfei Zhang Yumin Lyu Increased dicentric chromosome in peripheral lymphocytes is related to acute skin toxicity induced by radiotherapy in cancer patients Radiation Medicine and Protection Radiotherapy Radiation toxicity Cytogenetics Dicentric chromosome Radiosensitivity |
| title | Increased dicentric chromosome in peripheral lymphocytes is related to acute skin toxicity induced by radiotherapy in cancer patients |
| title_full | Increased dicentric chromosome in peripheral lymphocytes is related to acute skin toxicity induced by radiotherapy in cancer patients |
| title_fullStr | Increased dicentric chromosome in peripheral lymphocytes is related to acute skin toxicity induced by radiotherapy in cancer patients |
| title_full_unstemmed | Increased dicentric chromosome in peripheral lymphocytes is related to acute skin toxicity induced by radiotherapy in cancer patients |
| title_short | Increased dicentric chromosome in peripheral lymphocytes is related to acute skin toxicity induced by radiotherapy in cancer patients |
| title_sort | increased dicentric chromosome in peripheral lymphocytes is related to acute skin toxicity induced by radiotherapy in cancer patients |
| topic | Radiotherapy Radiation toxicity Cytogenetics Dicentric chromosome Radiosensitivity |
| url | http://www.sciencedirect.com/science/article/pii/S2666555724000728 |
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