Analysis of glutamate-dependent mechanism and optimization of fermentation conditions for poly-gamma-glutamic acid production by Bacillus subtilis SCP017-03.
Poly-gamma-glutamic acid (γ-PGA) is mainly synthesized by glutamate-dependent strains in the manufacturing industry. Therefore, understanding glutamate-dependent mechanisms is imperative. In this study, we first systematically analyzed the response of Bacillus subtilis SCP017-03 to glutamate additio...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0310556 |
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| author | Caiyun Wu Yutao Gou Shuai Jing Wei Li Fanglan Ge Jiao Li Yao Ren |
| author_facet | Caiyun Wu Yutao Gou Shuai Jing Wei Li Fanglan Ge Jiao Li Yao Ren |
| author_sort | Caiyun Wu |
| collection | DOAJ |
| description | Poly-gamma-glutamic acid (γ-PGA) is mainly synthesized by glutamate-dependent strains in the manufacturing industry. Therefore, understanding glutamate-dependent mechanisms is imperative. In this study, we first systematically analyzed the response of Bacillus subtilis SCP017-03 to glutamate addition by comparing transcriptomics and proteomics. The introduction of glutamate substantially altered gene expression within the central metabolic pathway of cellular carbon. Most genes in the pentose phosphate pathway (PPP), tricarboxylic acid (TCA) cycle, and energy-consuming phase of the glycolysis pathway (EMP) were down-regulated, whereas those in the energy-producing phase of glycolysis and those responsible for γ-PGA synthesis were up-regulated. Based on these findings, the fermentation conditions were optimized, and γ-PGA production was improved by incorporating oxygen carriers. In a batch-fed fermentor with glucose, the γ-PGA production reached 95.2 g/L, demonstrating its industrial production potential. This study not only elucidated the glutamate dependence mechanism of Bacillus subtilis but also identified a promising metabolic target for further enhancing γ-PGA production. |
| format | Article |
| id | doaj-art-c653b01505d74710959d4451750f4e74 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-c653b01505d74710959d4451750f4e742025-02-07T05:30:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01201e031055610.1371/journal.pone.0310556Analysis of glutamate-dependent mechanism and optimization of fermentation conditions for poly-gamma-glutamic acid production by Bacillus subtilis SCP017-03.Caiyun WuYutao GouShuai JingWei LiFanglan GeJiao LiYao RenPoly-gamma-glutamic acid (γ-PGA) is mainly synthesized by glutamate-dependent strains in the manufacturing industry. Therefore, understanding glutamate-dependent mechanisms is imperative. In this study, we first systematically analyzed the response of Bacillus subtilis SCP017-03 to glutamate addition by comparing transcriptomics and proteomics. The introduction of glutamate substantially altered gene expression within the central metabolic pathway of cellular carbon. Most genes in the pentose phosphate pathway (PPP), tricarboxylic acid (TCA) cycle, and energy-consuming phase of the glycolysis pathway (EMP) were down-regulated, whereas those in the energy-producing phase of glycolysis and those responsible for γ-PGA synthesis were up-regulated. Based on these findings, the fermentation conditions were optimized, and γ-PGA production was improved by incorporating oxygen carriers. In a batch-fed fermentor with glucose, the γ-PGA production reached 95.2 g/L, demonstrating its industrial production potential. This study not only elucidated the glutamate dependence mechanism of Bacillus subtilis but also identified a promising metabolic target for further enhancing γ-PGA production.https://doi.org/10.1371/journal.pone.0310556 |
| spellingShingle | Caiyun Wu Yutao Gou Shuai Jing Wei Li Fanglan Ge Jiao Li Yao Ren Analysis of glutamate-dependent mechanism and optimization of fermentation conditions for poly-gamma-glutamic acid production by Bacillus subtilis SCP017-03. PLoS ONE |
| title | Analysis of glutamate-dependent mechanism and optimization of fermentation conditions for poly-gamma-glutamic acid production by Bacillus subtilis SCP017-03. |
| title_full | Analysis of glutamate-dependent mechanism and optimization of fermentation conditions for poly-gamma-glutamic acid production by Bacillus subtilis SCP017-03. |
| title_fullStr | Analysis of glutamate-dependent mechanism and optimization of fermentation conditions for poly-gamma-glutamic acid production by Bacillus subtilis SCP017-03. |
| title_full_unstemmed | Analysis of glutamate-dependent mechanism and optimization of fermentation conditions for poly-gamma-glutamic acid production by Bacillus subtilis SCP017-03. |
| title_short | Analysis of glutamate-dependent mechanism and optimization of fermentation conditions for poly-gamma-glutamic acid production by Bacillus subtilis SCP017-03. |
| title_sort | analysis of glutamate dependent mechanism and optimization of fermentation conditions for poly gamma glutamic acid production by bacillus subtilis scp017 03 |
| url | https://doi.org/10.1371/journal.pone.0310556 |
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