Evaluation of cold atmospheric pressure plasma effects on Pseudomonas aeruginosa wound infection in a mouse model
Background and Objectives: Antibiotic resistance in microorganisms is a significant global health concern. Cold atmospheric plasma is an innovative and promising method for inactivating bacteria. This study aimed to evaluate the effects of cold plasma on Pseudomonas aeruginosa in a mouse wound infe...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2025-02-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/4453 |
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| author | Reyhaneh Shekari Gholamreza Zarrini Vahid Siahpoush Farzam Sheikhzadeh Hesari |
| author_facet | Reyhaneh Shekari Gholamreza Zarrini Vahid Siahpoush Farzam Sheikhzadeh Hesari |
| author_sort | Reyhaneh Shekari |
| collection | DOAJ |
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Background and Objectives: Antibiotic resistance in microorganisms is a significant global health concern. Cold atmospheric plasma is an innovative and promising method for inactivating bacteria. This study aimed to evaluate the effects of cold plasma on Pseudomonas aeruginosa in a mouse wound infection model.
Materials and Methods: The disk diffusion method was used to perform antibiograms after isolating the bacteria. A multidrug-resistant strain was then selected. The bactericidal activity of cold helium plasma was investigated in vitro. The optimal cold plasma conditions were determined in the laboratory, with a flow of 3 liters per minute and a power of 1.1 watts. These conditions were later used for in vivo evaluations.
Results: In a laboratory study, helium gas plasma treatment for 8 minutes reduced P. aeruginosa by 2.5 logs. In the in vivo study, plasma reduced the wound's microbial load in mice by 1.9 log. The antibiotic treatment group had a 1.2 log reduction. Both plasma and antibiotic therapies had similar effects on microbial inactivation.
Conclusion: The overall evaluation of wound healing time and pathological features showed that plasma was generally better than antibiotic treatment. Plasma can inactivate P. aeruginosa in wounds and accelerate wound healing.
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| format | Article |
| id | doaj-art-ca5949436d8146f48d9ad2aa2a5189e7 |
| institution | Kabale University |
| issn | 2008-3289 2008-4447 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj-art-ca5949436d8146f48d9ad2aa2a5189e72025-02-09T09:01:31ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472025-02-01171Evaluation of cold atmospheric pressure plasma effects on Pseudomonas aeruginosa wound infection in a mouse modelReyhaneh Shekari0Gholamreza Zarrini1Vahid Siahpoush2Farzam Sheikhzadeh Hesari3Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, IranDepartment of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, IranResearch Institute for Applied Physics and Astronomy, University of Tabriz, Tabriz, IranDepartment of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran Background and Objectives: Antibiotic resistance in microorganisms is a significant global health concern. Cold atmospheric plasma is an innovative and promising method for inactivating bacteria. This study aimed to evaluate the effects of cold plasma on Pseudomonas aeruginosa in a mouse wound infection model. Materials and Methods: The disk diffusion method was used to perform antibiograms after isolating the bacteria. A multidrug-resistant strain was then selected. The bactericidal activity of cold helium plasma was investigated in vitro. The optimal cold plasma conditions were determined in the laboratory, with a flow of 3 liters per minute and a power of 1.1 watts. These conditions were later used for in vivo evaluations. Results: In a laboratory study, helium gas plasma treatment for 8 minutes reduced P. aeruginosa by 2.5 logs. In the in vivo study, plasma reduced the wound's microbial load in mice by 1.9 log. The antibiotic treatment group had a 1.2 log reduction. Both plasma and antibiotic therapies had similar effects on microbial inactivation. Conclusion: The overall evaluation of wound healing time and pathological features showed that plasma was generally better than antibiotic treatment. Plasma can inactivate P. aeruginosa in wounds and accelerate wound healing. https://ijm.tums.ac.ir/index.php/ijm/article/view/4453Cold plasma;Multidrug-resistant;Wound healing antibacterial agents |
| spellingShingle | Reyhaneh Shekari Gholamreza Zarrini Vahid Siahpoush Farzam Sheikhzadeh Hesari Evaluation of cold atmospheric pressure plasma effects on Pseudomonas aeruginosa wound infection in a mouse model Iranian Journal of Microbiology Cold plasma; Multidrug-resistant; Wound healing antibacterial agents |
| title | Evaluation of cold atmospheric pressure plasma effects on Pseudomonas aeruginosa wound infection in a mouse model |
| title_full | Evaluation of cold atmospheric pressure plasma effects on Pseudomonas aeruginosa wound infection in a mouse model |
| title_fullStr | Evaluation of cold atmospheric pressure plasma effects on Pseudomonas aeruginosa wound infection in a mouse model |
| title_full_unstemmed | Evaluation of cold atmospheric pressure plasma effects on Pseudomonas aeruginosa wound infection in a mouse model |
| title_short | Evaluation of cold atmospheric pressure plasma effects on Pseudomonas aeruginosa wound infection in a mouse model |
| title_sort | evaluation of cold atmospheric pressure plasma effects on pseudomonas aeruginosa wound infection in a mouse model |
| topic | Cold plasma; Multidrug-resistant; Wound healing antibacterial agents |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/4453 |
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