Plasma from hypertensive pregnancy patients induce endothelial dysfunction even under atheroprotective shear stress
Abstract Preeclampsia (PE) is a challenge in maternal healthcare due to its complex nature, characterized by high blood pressure, protein in the urine, and damage to various organs. There is evidence linking PE to endothelial dysfunction (ED), triggered by substances released from an oxygen-deprived...
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Nature Portfolio
2025-02-01
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| author | Sarah Viana-Mattioli Miriam Helena Fonseca-Alaniz Iguaracy Pinheiro-de-Sousa Ricardo Rosa Junior Moises Henrique Mastella Ricardo de Carvalho Cavalli Valeria Cristina Sandrim |
| author_facet | Sarah Viana-Mattioli Miriam Helena Fonseca-Alaniz Iguaracy Pinheiro-de-Sousa Ricardo Rosa Junior Moises Henrique Mastella Ricardo de Carvalho Cavalli Valeria Cristina Sandrim |
| author_sort | Sarah Viana-Mattioli |
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| description | Abstract Preeclampsia (PE) is a challenge in maternal healthcare due to its complex nature, characterized by high blood pressure, protein in the urine, and damage to various organs. There is evidence linking PE to endothelial dysfunction (ED), triggered by substances released from an oxygen-deprived placenta. Previous in vitro studies have not considered the impact of in vivo elements, such as the different patterns of blood flow, and laminar (LSS) vs. oscillatory (OSS) shear stress, on the development of ED. We investigated the impact of plasma from healthy pregnant women (HP), subjects with gestational hypertension (GH), and PE patients on global gene expression of human coronary endothelial cells (HCAECs) under LSS and OSS. Our findings revealed a unique transcriptional profile of endothelial cells induced by plasma incubation in LSS. Notably, OSS resulted in similar transcriptomes irrespective of plasma treatment. Under LSS, GH plasma resulted in a proliferative profile, whereas PE plasma was linked to pro-inflammatory and antioxidant profiles compared to HP plasma. Our findings demonstrate that shear stress levels influence the endothelial cell transcriptome in response to plasma from hypertensive pregnancy patients. Both PE and GH can induce endothelial dysfunction under atheroprotective LSS, with a more significant effect observed with PE-derived plasma. |
| format | Article |
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| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-cadba5d728924d2ba523a7c00d5d11ee2025-02-09T12:31:25ZengNature PortfolioScientific Reports2045-23222025-02-0115111510.1038/s41598-025-88902-8Plasma from hypertensive pregnancy patients induce endothelial dysfunction even under atheroprotective shear stressSarah Viana-Mattioli0Miriam Helena Fonseca-Alaniz1Iguaracy Pinheiro-de-Sousa2Ricardo Rosa Junior3Moises Henrique Mastella4Ricardo de Carvalho Cavalli5Valeria Cristina Sandrim6Department of Biophysics and Pharmacology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP)Laboratorio de Genetica e Cardiologia Molecular, Instituto do Coracao (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao PauloLaboratorio de Genetica e Cardiologia Molecular, Instituto do Coracao (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao PauloLaboratorio de Genetica e Cardiologia Molecular, Instituto do Coracao (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao PauloDepartment of Biophysics and Pharmacology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP)Department of Gynecology and Obstetrics, Hospital das Clínicas, Medical School of Ribeirão Preto, University of São PauloDepartment of Biophysics and Pharmacology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP)Abstract Preeclampsia (PE) is a challenge in maternal healthcare due to its complex nature, characterized by high blood pressure, protein in the urine, and damage to various organs. There is evidence linking PE to endothelial dysfunction (ED), triggered by substances released from an oxygen-deprived placenta. Previous in vitro studies have not considered the impact of in vivo elements, such as the different patterns of blood flow, and laminar (LSS) vs. oscillatory (OSS) shear stress, on the development of ED. We investigated the impact of plasma from healthy pregnant women (HP), subjects with gestational hypertension (GH), and PE patients on global gene expression of human coronary endothelial cells (HCAECs) under LSS and OSS. Our findings revealed a unique transcriptional profile of endothelial cells induced by plasma incubation in LSS. Notably, OSS resulted in similar transcriptomes irrespective of plasma treatment. Under LSS, GH plasma resulted in a proliferative profile, whereas PE plasma was linked to pro-inflammatory and antioxidant profiles compared to HP plasma. Our findings demonstrate that shear stress levels influence the endothelial cell transcriptome in response to plasma from hypertensive pregnancy patients. Both PE and GH can induce endothelial dysfunction under atheroprotective LSS, with a more significant effect observed with PE-derived plasma.https://doi.org/10.1038/s41598-025-88902-8Endothelial dysfunctionPregnancyHypertensionShear stressPreeclampsia |
| spellingShingle | Sarah Viana-Mattioli Miriam Helena Fonseca-Alaniz Iguaracy Pinheiro-de-Sousa Ricardo Rosa Junior Moises Henrique Mastella Ricardo de Carvalho Cavalli Valeria Cristina Sandrim Plasma from hypertensive pregnancy patients induce endothelial dysfunction even under atheroprotective shear stress Scientific Reports Endothelial dysfunction Pregnancy Hypertension Shear stress Preeclampsia |
| title | Plasma from hypertensive pregnancy patients induce endothelial dysfunction even under atheroprotective shear stress |
| title_full | Plasma from hypertensive pregnancy patients induce endothelial dysfunction even under atheroprotective shear stress |
| title_fullStr | Plasma from hypertensive pregnancy patients induce endothelial dysfunction even under atheroprotective shear stress |
| title_full_unstemmed | Plasma from hypertensive pregnancy patients induce endothelial dysfunction even under atheroprotective shear stress |
| title_short | Plasma from hypertensive pregnancy patients induce endothelial dysfunction even under atheroprotective shear stress |
| title_sort | plasma from hypertensive pregnancy patients induce endothelial dysfunction even under atheroprotective shear stress |
| topic | Endothelial dysfunction Pregnancy Hypertension Shear stress Preeclampsia |
| url | https://doi.org/10.1038/s41598-025-88902-8 |
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