Comparative analysis of viability, proliferation, and mineralization potential of human pulp and osteoblastic cells exposed to different bioceramic endodontic sealers
Background: The present study aimed to compare the viability, proliferation, and mineralization potential of human dental pulp cells (hDPCs) and osteoblasts cell line (Saos-2) after exposure to AH Plus® Bioceramic (AHP-B), Bio-C® Sealer (BIO-C), NeoMTA Plus® (NEOMTA-P), and MTA-FILLAPEX® endodontic...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426825000107 |
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| author | Marcos Coelho Santiago Gustavo Henrique de Oliveira Salles Gustavo Gomes de Lima Laudimar Alves de Oliveira Loise Pedrosa Salles |
| author_facet | Marcos Coelho Santiago Gustavo Henrique de Oliveira Salles Gustavo Gomes de Lima Laudimar Alves de Oliveira Loise Pedrosa Salles |
| author_sort | Marcos Coelho Santiago |
| collection | DOAJ |
| description | Background: The present study aimed to compare the viability, proliferation, and mineralization potential of human dental pulp cells (hDPCs) and osteoblasts cell line (Saos-2) after exposure to AH Plus® Bioceramic (AHP-B), Bio-C® Sealer (BIO-C), NeoMTA Plus® (NEOMTA-P), and MTA-FILLAPEX® endodontic sealers (MTA-F). Methods: All materials were prepared according to the manufacturer's instructions. Before exposing the cells, we measured the release of calcium ions (Ca2+) from the dental materials to the culture media once Ca2+ can trigger signaling pathways. After that, hDPCs and Saos-2 were exposed to the sealers for MTT assay to assess the cell viability and wound healing to evaluate the cell proliferation. To investigate the potential of mineralization, we assessed the alkaline phosphatase activity and calcium deposition by Alizarin red staining. Statistical analysis was performed using Two-way ANOVA for calcium release and wound healing assays and One-way ANOVA for other assays, with post-test Bonferroni correction. The results were significant when p < 0.05. Results: The sealers released diverse concentrations of calcium at different times. The hDPCs viability and proliferation were low in the AHP-B group at 24h of exposure (NEOMTA-P ∼ BIO-C ∼ CT > AHP-B > MTA-F), distinct from the osteoblastic cells (NEOMTA-P ∼ AHP-B ∼ CT > BIO-C > MTA-F) and (proliferation: AHP-B > NEOMTA-P ∼ CT > BIO-C > MTA-F). The ALP activity, an early marker of osteogenesis, was higher in hDPCs exposed to NEOMTA-P, while the osteoblastic cells showed higher ALP when exposed to AHP-B. Conclusion: AHP-B, NEOMTA-P, and BIO-C stimulated osteogenesis in hDPCs and Saos-2 cells, with marked differences between groups. AHP-B showed an improved early stimulation of osteoblastic cells, while hDPCs were more responsive to NEOMTA-P. |
| format | Article |
| id | doaj-art-cb425ba566e14726a998654c57b65809 |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-cb425ba566e14726a998654c57b658092025-02-09T05:00:16ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682025-01-01151191198Comparative analysis of viability, proliferation, and mineralization potential of human pulp and osteoblastic cells exposed to different bioceramic endodontic sealersMarcos Coelho Santiago0Gustavo Henrique de Oliveira Salles1Gustavo Gomes de Lima2Laudimar Alves de Oliveira3Loise Pedrosa Salles4University of Brasilia, Faculty of Health Sciences, Post-Graduation Program in Dentistry, Brasília, DF, BrazilUniversity of Planalto Central, Medical School, Brasília, DF, BrazilUniversity of Brasilia, Faculty of Health Sciences, Post-Graduation Program in Dentistry, Brasília, DF, BrazilUniversity of Brasilia, Faculty of Health Sciences, Post-Graduation Program in Dentistry, Brasília, DF, BrazilUniversity of Brasilia, Faculty of Health Sciences, Post-Graduation Program in Dentistry, Brasília, DF, Brazil; Corresponding author. University of Brasília (UnB), Faculty of Health Sciences, Department of Dentistry. Campus Universitário Darcy Ribeiro, Asa Norte, Brasília, DF, 70910-900, Brazil.Background: The present study aimed to compare the viability, proliferation, and mineralization potential of human dental pulp cells (hDPCs) and osteoblasts cell line (Saos-2) after exposure to AH Plus® Bioceramic (AHP-B), Bio-C® Sealer (BIO-C), NeoMTA Plus® (NEOMTA-P), and MTA-FILLAPEX® endodontic sealers (MTA-F). Methods: All materials were prepared according to the manufacturer's instructions. Before exposing the cells, we measured the release of calcium ions (Ca2+) from the dental materials to the culture media once Ca2+ can trigger signaling pathways. After that, hDPCs and Saos-2 were exposed to the sealers for MTT assay to assess the cell viability and wound healing to evaluate the cell proliferation. To investigate the potential of mineralization, we assessed the alkaline phosphatase activity and calcium deposition by Alizarin red staining. Statistical analysis was performed using Two-way ANOVA for calcium release and wound healing assays and One-way ANOVA for other assays, with post-test Bonferroni correction. The results were significant when p < 0.05. Results: The sealers released diverse concentrations of calcium at different times. The hDPCs viability and proliferation were low in the AHP-B group at 24h of exposure (NEOMTA-P ∼ BIO-C ∼ CT > AHP-B > MTA-F), distinct from the osteoblastic cells (NEOMTA-P ∼ AHP-B ∼ CT > BIO-C > MTA-F) and (proliferation: AHP-B > NEOMTA-P ∼ CT > BIO-C > MTA-F). The ALP activity, an early marker of osteogenesis, was higher in hDPCs exposed to NEOMTA-P, while the osteoblastic cells showed higher ALP when exposed to AHP-B. Conclusion: AHP-B, NEOMTA-P, and BIO-C stimulated osteogenesis in hDPCs and Saos-2 cells, with marked differences between groups. AHP-B showed an improved early stimulation of osteoblastic cells, while hDPCs were more responsive to NEOMTA-P.http://www.sciencedirect.com/science/article/pii/S2212426825000107Calcium silicateDental materialsDental pulpEndodonticsOsteoblastsRoot canal obturation |
| spellingShingle | Marcos Coelho Santiago Gustavo Henrique de Oliveira Salles Gustavo Gomes de Lima Laudimar Alves de Oliveira Loise Pedrosa Salles Comparative analysis of viability, proliferation, and mineralization potential of human pulp and osteoblastic cells exposed to different bioceramic endodontic sealers Journal of Oral Biology and Craniofacial Research Calcium silicate Dental materials Dental pulp Endodontics Osteoblasts Root canal obturation |
| title | Comparative analysis of viability, proliferation, and mineralization potential of human pulp and osteoblastic cells exposed to different bioceramic endodontic sealers |
| title_full | Comparative analysis of viability, proliferation, and mineralization potential of human pulp and osteoblastic cells exposed to different bioceramic endodontic sealers |
| title_fullStr | Comparative analysis of viability, proliferation, and mineralization potential of human pulp and osteoblastic cells exposed to different bioceramic endodontic sealers |
| title_full_unstemmed | Comparative analysis of viability, proliferation, and mineralization potential of human pulp and osteoblastic cells exposed to different bioceramic endodontic sealers |
| title_short | Comparative analysis of viability, proliferation, and mineralization potential of human pulp and osteoblastic cells exposed to different bioceramic endodontic sealers |
| title_sort | comparative analysis of viability proliferation and mineralization potential of human pulp and osteoblastic cells exposed to different bioceramic endodontic sealers |
| topic | Calcium silicate Dental materials Dental pulp Endodontics Osteoblasts Root canal obturation |
| url | http://www.sciencedirect.com/science/article/pii/S2212426825000107 |
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